Characterization of the T cell receptor repertoire causing collagen arthritis in mice

Collagen type II-induced arthritis (CIA) is generated in susceptible rodent strains by intradermal injections of homologous or heterologous native type II collagen in complete Freund's adjuvant. Symptoms of CIA are analogous to those of the human autoimmune disease, rheumatoid arthritis. CIA is a model system for T cell-mediated autoimmune disease. To study the T cell receptor (TCR) repertoire of bovine type II-specific T cells that may be involved in the pathogenesis of CIA in DBA/1Lac.J (H-2q) mice, 13 clonally distinct T cell hybridomas specific for bovine type II collagen have been established and the alpha and beta chains of their TCRs have been analyzed. These T cell hybridomas recognize epitopes that are shared by type II collagens from distinct species and not by type I collagens, and exhibit a highly restricted TCR-alpha/beta repertoire. The alpha chains of the TCRs employ three V alpha gene subfamilies (V alpha 11, V alpha 8, and V alpha 22) and four J alpha gene segments (J alpha 42, J alpha 24, J alpha 37, and J alpha 32). The V alpha 22 is a newly identified subfamily consisting of approximately four to six members, and exhibits a high degree of polymorphism among four mouse strains of distinct V alpha haplotypes. In addition, the beta chains of the TCRs employ three V beta gene subfamilies (V beta 8, V beta 1, and V beta 6), however the V beta 8.2 gene segment is preferentially utilized (58.3%). In contrast, the J beta gene segment usage is more heterogeneous. On the basis of the highly limited TCR-alpha/beta repertoire of the TCRs of the panel of bovine type II-specific T cell hybrid clones, a significant reduction (60%) of the incidence of arthritis in DBA/1Lac.J mice is accomplished by the use of anti-V beta 8.2 antibody therapy

IMPROVING IRRIGATION WATER ALLOCATION EFFICIENCY USING ALTERNATIVE POLICY OPTIONS IN EGYPT

This study provides an empirical perspective on alternative irrigation policies for allocating limited water to agricultural production in Egypt. Positive mathematical programming is used for model calibration. Three policy options for Egypt are tested: water pricing, water complementary input factor taxes, and output taxes. The results of the research show that: 1) water pricing needs to be much higher than the recovery cost in order to be effective in limiting irrigation water use; 2) at a higher tax rate, fertilizer and energy taxes are effective in reducing the irrigation water demand while maintaining adequate welfare levels; 3) a pesticide tax is less effective than fertilizer or energy taxes; and 4) an output tax on sugar cane and rice would decrease irrigation water demand substantially while allocating land to other crops which are less water intensive and have higher market values.Resource /Energy Economics and Policy,

The conversation: developing confidence to provide end of life care in Salford nursing homes

The study was funded by the Burdett Trust for Nursing and partly by Salford Primary Care Trust. A realistic evaluation design was used to collect data using a range of approaches, from before and after surveys of confidence in delivering end of life care, to participant observation and interviews. A total of 43 people were interviewed involving both staff, residents and relatives. Key Messages a) Significant resources are needed to engage staff, residents and relatives/carers with the idea of advance care planning b) Care home staff are optimistic about involving residents and relatives in planning care at the end of life and some relatives become very involved in care c) Clearly registered nurses and other care home workers such as care assistants have different roles, but the overlap between these and the appropriate boundaries would benefit from further work d) Talking to residents and relatives about their feelings and wishes for care at the end of life remains especially difficult, but education and training in key skills and knowledge can engender both ability and motivation e) Care homes need strong and well-informed leadership in order to implement the Gold Standards Framework f) Placing a relative in a care home involves strain and an ability to compromise ‘there’s no perfect place’ g) Advance care planning can reduce the distress and the number of inappropriate hospital admissions, but is challenging in the face of staff rotation and out of hours medical staff being unpredictable h) The principles of the Gold Standards Framework are widely seen as sensible, but clinical challenges include diagnosing and predicting dying trajectories, especially in heart failure, chronic pulmonary disease and dementia i) A particular concern of staff is how to approach nutrition and hydration as frailty and death approach j) Communicating about diagnosis and especially prognosis with residents who lack capacity is an increasing problem k) Natural justice suggests that resources should be allocated to the general standardisation of a good quality of care at the end of life in ALL care homes whatever their Care Quality Commission ratin

Strategic Policy Options to Improve Irrigation Water Allocation Efficiency: Analysis on Egypt and Morocco

For a number of reasons government the world over have been unwilling to use water pricing to achieve water use efficiency. This research addresses questions of what policy alternatives to water pricing might improve irrigation water allocation efficiency. An empirical framework is provided to compare irrigation policies for allocating scarce water to agricultural production in Egypt and Morocco. Partial equilibrium agricultural sector models specific to Egypt and Morocco were employed for policy tests. Positive Mathematical Programming (PMP) was used to calibrate the models. Water pricing policy, water complementary input factor tax policy, and output tax policy are tested. Results suggest that effective policy depends on the social, economic, and environmental contexts of specific regions. The results for both countries demonstrate that some of the alternative irrigation policies can work towards directing cropping decisions to less water intensive crops and also generating revenues for governments in situations where governments choose not to price water.Resource /Energy Economics and Policy,

Numerical investigation of high-pressure combustion in rocket engines using Flamelet/Progress-variable models

The present paper deals with the numerical study of high pressure LOx/H2 or LOx/hydrocarbon combustion for propulsion systems. The present research effort is driven by the continued interest in achieving low cost, reliable access to space and more recently, by the renewed interest in hypersonic transportation systems capable of reducing time-to-destination. Moreover, combustion at high pressure has been assumed as a key issue to achieve better propulsive performance and lower environmental impact, as long as the replacement of hydrogen with a hydrocarbon, to reduce the costs related to ground operations and increase flexibility. The current work provides a model for the numerical simulation of high- pressure turbulent combustion employing detailed chemistry description, embedded in a RANS equations solver with a Low Reynolds number k-omega turbulence model. The model used to study such a combustion phenomenon is an extension of the standard flamelet-progress-variable (FPV) turbulent combustion model combined with a Reynolds Averaged Navier-Stokes equation Solver (RANS). In the FPV model, all of the thermo-chemical quantities are evaluated by evolving the mixture fraction Z and a progress variable C. When using a turbulence model in conjunction with FPV model, a probability density function (PDF) is required to evaluate statistical averages of chemical quantities. The choice of such PDF must be a compromise between computational costs and accuracy level. State- of-the-art FPV models are built presuming the functional shape of the joint PDF of Z and C in order to evaluate Favre-averages of thermodynamic quantities. The model here proposed evaluates the most probable joint distribution of Z and C without any assumption on their behavior.Comment: presented at AIAA Scitech 201

Noncoherent Space-Time Coding: An Algebraic Perspective

Cataloged from PDF version of article.The design of space–time signals for noncoherent block-fading channels where the channel state information is not known a priori at the transmitter and the receiver is considered. In particular, a new algebraic formulation for the diversity advantage design criterion is developed. The new criterion encompasses, as a special case, the well-known diversity advantage for unitary space–time signals and, more importantly, applies to arbitrary signaling schemes and arbitrary channel distributions. This criterion is used to establish the optimal diversity-versus-rate tradeoff for training based schemes in block-fading channels. Our results are then specialized to the class of affine space–time signals which allows for a low complexity decoder. Within this class, space–time constellations based on the threaded algebraic space–time (TAST) architecture are considered. These constellations achieve the optimal diversity-versus-rate tradeoff over noncoherent block-fading channels and outperform previously proposed codes in the considered scenarios as demonstrated by the numerical results. Using the analytical and numerical results developed in this paper, nonunitary space–time codes are argued to offer certain advantages in block-fading channels where the appropriate use of coherent space–time codes is shown to offer a very efficient solution to the noncoherent space–time communication paradigm