Exploration of Ludruk as Potential Icon in Indonesia Show Business for Millenial Generation

The purpose of the research is to understand why Ludruk as a traditional cultural art is not in demand by young, millennial generations. And further can give suggestions how to make Ludruk become world show icon in Surabaya. The qualitative approach was conducted in this research. The results of the study was the inability of managers and artists to produce and features ludruk products art that meet consumers expectation caused by reluctance of ludruk artists itself to develop and improve existing art products. Paradoxes of creativity identified in this research. Showing that even Ludruk is part of creative industry and need higher improvisation, but the senior player feel reluctant to innovate the show.It need support of government and CSR from private company to helps them

Abstract 5112: Proteomic investigation of melphalan resistance in multiple myeloma to support selection of combination therapy

Abstract Introduction: Proteomic analyses using iTRAQ and reaction monitoring mass spectrometry are used to examine the mechanism of melphalan resistance and build an assessment platform to enable future selection of combination therapy in patients. Experimental Procedures: Drug IC50 values and interactions are assessed using cell viability measurements. Protein expression analysis using isobaric tags for relative and absolute quantification (iTRAQ) was used to compare melphalan resistant cell lines (8226/LR5 and U266/LR6) to their isogenic, naïve counterparts (RPMI-8226, U266). In this study, iTRAQ was used to identify proteins that have changed in expression level during the development of melphalan resistance in multiple myeloma (MM). In addition to manual selection of differentially expressed proteins, the resulting quantitative data were coalesced into pathway models using Metacore, GeneGO for further analysis. For targeted protein detection and quantification, liquid chromatography coupled to multiple reaction monitoring mass spectrometry (LC-MRM) assays are developed and implemented. Data Summary: Melphalan resistant cells are more susceptible to other chemotherapy agents, including steroids, proteasome inhibitors, and geldanamycin derivatives, than their isogenic, naïve counterparts. To investigate the differences, iTRAQ showed numerous changes in protein expression, including redox stress components, mitochondrial proteins, and heat shock proteins (HSPs). LC-MRM assays were developed for proteins detected in the iTRAQ experiments that are current chemotherapy targets, e.g. HSPs and proteasome components. Furthermore, assays have also been developed for additional protein targets of other chemotherapy agents, e.g. topoisomerases and the glucocorticoid receptor, that were not detected in the iTRAQ results. LC-MRM was then used to examine the expression of these proteins in the four model cell lines listed above and in the naïve cells following the onset of drug treatment. Endogenous proteins were quantified in less than 10,000 cells. These assays can be combined to develop an assessment tool to help elucidate the mechanisms of drug resistance and enable the assessment of myeloma cells from patients’ bone marrow aspirates. Conclusions: Quantification of the proteomes of myeloma cell lines using iTRAQ provided insights into the mechanism of melphalan resistance in multiple myeloma. Investigation of the expression of these proteins and other drug targets in myeloma cell lines has produced an LC-MRM platform that can be translated for patient assessment to assist in selection of chemotherapy combinations. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 5112. doi:10.1158/1538-7445.AM2011-5112</jats:p

Anti-amyloid activity of amino acid functionalized magnetic nanoparticles on αLactalbumin aggregation

Protein amyloid aggregation involves structural changes in native protein conformers and the formation of amyloid fibrils that accumulate in deposits in the human body. This study explores the effect of magnetic nanoparticles functionalized with amino acids (aaMNPs)—cysteine (Cys), poly-L-lysine (PLL), or proline (Pro)—on the amyloid aggregation of α-lactalbumin (αLA) and its amyloid fibrils (LAF). Our results from thioflavin T fluorescence assay (ThT), atomic force microscopy (AFM), and infrared spectroscopy revealed that the studied aaMNPs inhibit αLA fibrillization and destruct LAF in a concentration-dependent manner. The type of amino acid used for nanoparticle functionalization significantly influences the anti-amyloid efficacy. ProMNPs exhibit the highest inhibitory activity, with the timing of their addition being crucial Conversely, CysMNPs demonstrate the highest destructing activity. AFM image analysis through grain mapping was employed to quantify the anti-amyloid effects of aaMNPs. Cytotoxicity testing on kidney cells identified PLLMNPs as the only cytotoxic nanoparticles in our study. These findings clarify the mechanisms of inhibition and destruction of LAF in the presence of aaMNPs, which could inform the design of nanoparticles for therapeutic purposes in the future