An efficient Performance-Based Seismic Design of Reinforced Concrete Frames

In this paper, a practical method is developed for performance-based design of RC structures subjected to seismic excitations. More efficient design is obtained by redistributing material from strong to weak parts of a structure until a state of uniform deformation or damage prevails. By applying the design algorithm on 5, 10 and 15-storey RC frames, the efficiency of the proposed method is initially demonstrated for specific synthetic and real seismic excitations. The results indicate that, for similar structural weight, designed structures experience up to 30% less global damage compared with code-based design frames. The method is then developed to consider multiple performance objectives and deal with seismic design of RC structures for a design spectrum. The results show that the proposed method is very efficient at controlling performance parameters and improving structural behaviour of RC frames

Optimum seismic design of reinforced concrete frames according to Eurocode 8 and fib Model Code 2010

Traditional seismic design, like the one adopted in Eurocode 8 (EC8), is force-based and examining a single level of seismic action. In order to provide improved control of structural damage for different levels of seismic action, the new fib Model Code 2010 (MC2010) includes a fully fledged displacement-based and performance-based seismic design methodology. However, the level of complexity and computational effort of the MC2010 methodology is significantly increased. Hence, the use of automated optimization techniques for obtaining cost-effective design solutions becomes appealing if not necessary. This study employs genetic algorithms to derive and compare optimum seismic design solutions of reinforced concrete frames according to EC8 and MC2010. This is important because MC2010 is meant to serve as a basis for future seismic design codes. It is found that MC2010 drives to more cost-effective solutions than EC8 for regions of low seismicity and better or similar costs for regions of moderate seismicity. For high-seismicity regions, MC2010 may yield similar or increased structural costs. This depends strongly on the provisions adopted for selecting the set of ground motions. In all cases, MC2010 provides enhanced control of structural damage

Contribution to sustainable seismic design of reinforced concrete members through embodied CO2 emissions optimization

The embodied CO2 emissions of reinforced concrete (RC) structures can be significantly reduced by structural optimization that maximizes structural efficiency. Previous studies dealing with design of RC structures for minimum CO2 emissions do not address seismic design provisions. This is the case despite the fact that in many countries around the world, including most of the top-10 countries in CO2 emissions from cement production, RC structures have to be designed against earthquake hazard. To fill a part of this gap, this study, using exhaustive search, examines optimum designs of RC beam and column members for minimum embodied CO2 emissions according to Eurocode-8 for all ductility classes and compares them with optimum designs based on material cost. It is shown that seismic designs for minimum CO2 footprint lead to less CO2 emissions but are more expensive than minimum cost designs. Their differences strongly depend on the assumed values of the environmental impact of reinforcing steel and concrete materials. Furthermore, it is concluded that seismic design for high ductility classes can drive to significant reductions in embodied CO2 emissions

Label-Free Optical Sensing and Medical Grade Resins: An Advanced Approach to Investigate Cell–Material Interaction and Biocompatibility

: The Corning Epic® label-free (ELF) system is an innovative technology widely used in drug discovery, immunotherapy, G-protein-associated studies, and biocompatibility tests. Here, we challenge the use of ELF to further investigate the biocompatibility of resins used in manufacturing of blood filters, a category of medical devices representing life-saving therapies for the increasing number of patients with kidney failure. The biocompatibility assays were carried out by developing a cell model aimed at mimicking the clinical use of the blood filters and complementing the existing cytotoxicity assay requested by ISO10993-5. Experiments were performed by putting fibroblasts in both direct contact with two types of selected resins, and indirect contact by means of homemade customized well inserts that were precisely designed and developed for this technology. For both types of contact, fibroblasts were cultured in medium and human plasma. ELF tests confirmed the biocompatibility of both resins, highlighting a statistically significant different biological behavior of a polyaromatic resin compared to control and ion-exchanged resin, when materials were in indirect contact and soaking with plasma. Overall, the ELF test is able to mimic clinical scenarios and represents a promising approach to investigate biocompatibility, showing peculiar biological behaviors and suggesting the activation of specific intracellular pathways

Novel bioprinted 3D model to human fibrosis investigation

Fibrosis is shared in multiple diseases with progressive tissue stiffening, organ failure and limited therapeutic options. This unmet need is also due to the lack of adequate pre-clinical models to mimic fibrosis and to be challenged novel by anti-fibrotic therapeutic venues. Here using bioprinting, we designed a novel 3D model where normal human healthy fibroblasts have been encapsulated in type I collagen. After stimulation by Transforming Growth factor beta (TGFβ), embedded cells differentiated into myofibroblasts and enhanced the contractile activity, as confirmed by the high level of α − smooth muscle actin (αSMA) and F-actin expression. As functional assays, SEM analysis revealed that after TGFβ stimulus the 3D microarchitecture of the scaffold was dramatically remolded with an increased fibronectin deposition with an abnormal collagen fibrillar pattern. Picrius Sirius Red staining additionally revealed that TGFβ stimulation enhanced of two logarithm the collagen fibrils neoformation in comparison with control. These data indicate that by bioprinting technology, it is possible to generate a reproducible and functional 3D platform to mimic fibrosis as key tool for drug discovery and impacting on animal experimentation and reducing costs and time in addressing fibrosis

Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance

The resorption rate of autologous fat transfer (AFT) is 40-60% of the implanted tissue, requiring new surgical strategies for tissue reconstruction. We previously demonstrated in a rabbit model that AFT may be empowered by adipose-derived mesenchymal stromal/stem cells (AD-MSCs), which improve graft persistence by exerting proangiogenic/anti-inflammatory effects. However, their fate after implantation requires more investigation. We report a xenograft model of adipose tissue engineering in which NOD/SCID mice underwent AFT with/without human autologous AD-MSCs and were monitored for 180 days (d). The effect of AD-MSCs on AFT grafting was also monitored by evaluating the expression of CD31 and F4/80 markers. Green fluorescent protein-positive AD-MSCs (AD-MSC-GFP) were detected in fibroblastoid cells 7 days after transplantation and in mature adipocytes at 60 days, indicating both persistence and differentiation of the implanted cells. This evidence also correlated with the persistence of a higher graft weight in AFT-AD-MSC compared to AFT alone treated mice. An observation up to 180 d revealed a lower resorption rate and reduced lipidic cyst formation in the AFT-AD-MSC group, suggesting a long-term action of AD-MSCs in support of AFT performance and an anti-inflammatory/proangiogenic activity. Together, these data indicate the protective role of adipose progenitors in autologous AFT tissue resorption

Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance

The resorption rate of autologous fat transfer (AFT) is 40–60% of the implanted tissue, requiring new surgical strategies for tissue reconstruction. We previously demonstrated in a rabbit model that AFT may be empowered by adipose-derived mesenchymal stromal/stem cells (AD-MSCs), which improve graft persistence by exerting proangiogenic/anti-inflammatory effects. However, their fate after implantation requires more investigation. We report a xenograft model of adipose tissue engineering in which NOD/SCID mice underwent AFT with/without human autologous AD-MSCs and were monitored for 180 days (d). The effect of AD-MSCs on AFT grafting was also monitored by evaluating the expression of CD31 and F4/80 markers. Green fluorescent protein-positive AD-MSCs (AD-MSC-GFP) were detected in fibroblastoid cells 7 days after transplantation and in mature adipocytes at 60 days, indicating both persistence and differentiation of the implanted cells. This evidence also correlated with the persistence of a higher graft weight in AFT-AD-MSC compared to AFT alone treated mice. An observation up to 180 d revealed a lower resorption rate and reduced lipidic cyst formation in the AFT-AD-MSC group, suggesting a long-term action of AD-MSCs in support of AFT performance and an anti-inflammatory/proangiogenic activity. Together, these data indicate the protective role of adipose progenitors in autologous AFT tissue resorption