The first complete genome sequence of narcissus latent virusfrom Narcissus

AbstractThe complete sequence of a narcissus virus isolated from the Netherlands (Narv-NL) was determined to be 8172 nucleotidesin length with an open reading frame encoding for 2624 amino acids. Phylogenetic analysis indicates that Narv-NLis clustered with high confidence among representative members from the genus Macluravirus, including artichoke latentvirus (ArLV) and Chinese yam necrotic mosaic virus (CYNMV). Sequence analyses indicated Narv-NL shares 67%-69%nucleotide and 51%-68% amino acid sequence identity with ArLV and CYNMV either in the complete ORF or the coatprotein (CP) gene, whereas it had 81%-99 % nucleotide and 80%-99 % amino acid sequence identity with the correspondingCP sequences of narcissus latent virus (NLV) isolates, suggesting that Narv-NL is a member of NLV. To our knowledge,this is the first report of the complete sequence of a NLV isolate