Searching for γ\gamma-ray Counterparts to Very Faint X-Ray Transient Neutron Star binaries

Very faint X-ray transients (VFXTs) are a group of X-ray binaries with low luminosities, the peak X-ray luminosities during their outbursts being only 10$^{34}$--10$^{36}$ erg\,s$^{-1}$. Using the $\gamma$-ray data obtained with the Large Area Telescope (LAT) onboard {\it Fermi Gamma-Ray Space Telescope}, we investigate their possible nature of containing rotation-powered pulsars, or more specifically being transitional millisecond pulsars (MSPs). Among more than 40 known VFXTs, we select 12 neutron star systems. We analyze the LAT data for the fields of the 12 VFXTs in 0.2--300 GeV energy range, but do not find any counterparts likely detected by {\it Fermi}. We obtain the luminosity upper limits for the 12 sources. While the distances to the sources are largely uncertain, the upper limits are comparable to the luminosities of two transitional systems PSR J1023$-$0038 and XSS J12270$-$4859. From our study, we conclude that no evidence is found at $\gamma$-rays for the suggestion that some of VFXTs could contain rotation-powered MSPs (or be transitional MSP systems).Comment: 14 pages, 3 figure

Unveiling the metabolic fate of monosaccharides in cell membranes with glycomic and glycoproteomic analyses.

Cell membrane protein glycosylation is dependent on the metabolic state of the cell as well as exogenous nutrients available. Although the metabolism and interconversion of monosaccharides have been well-studied, their incorporation into cell surface glycans and their corresponding glycoproteins remains relatively unknown. In this study, we developed a method to investigate quantitatively the incorporation pathways of dietary saccharides into specific glycans and glycoproteins on the cell membrane by treating intestinal Caco-2 and hepatic KKU-M213 cells with 13C-labeled monosaccharides and characterizing the resulting cell surface glycans and glycopeptides by LC-MS/MS. Time-course studies using uniformly labeled glucose revealed that the rate of incorporation was both glycan-specific and protein-dependent. Comparative studies using different dietary saccharides and multiple cell lines revealed the variance of monosaccharide utilization and interconversion in different tissues and organisms. The robust isotope-labeling and glycan profiling methods can provide a useful tool for differentiating glycosylation pathways and enhance the understanding of how dietary sugar intake affects health

Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation.

Given that unnatural sugar expression is metabolically achieved, the kinetics and disposition of incorporation can lend insight into the temporal and localization preferences of sialylation across the cell surface. However, common detection schemes lack the ability to detail the molecular diversity and distribution of target moieties. Here we employed a mass spectrometric approach to trace the placement of azido sialic acids on membrane glycoconjugates, which revealed substantial variations in incorporation efficiencies between N-/O-glycans, glycosites, and glycosphingolipids. To further explore the propensity for sialylation, we subsequently mapped the native glycome of model epithelial cell surfaces and illustrate that while glycosylation sites span broadly across the extracellular region, a higher number of heterogeneous glycoforms occur on sialylated sites closest to the transmembrane domain. Beyond imaging techniques, this integrative approach provides unprecedented details about the frequency and structure-specific distribution of cell surface sialylation, a critical feature that regulates cellular interactions and homeostatic pathways

Infection-generated electric field in gut epithelium drives bidirectional migration of macrophages.

Many bacterial pathogens hijack macrophages to egress from the port of entry to the lymphatic drainage and/or bloodstream, causing dissemination of life-threatening infections. However, the underlying mechanisms are not well understood. Here, we report that Salmonella infection generates directional electric fields (EFs) in the follicle-associated epithelium of mouse cecum. In vitro application of an EF, mimicking the infection-generated electric field (IGEF), induces directional migration of primary mouse macrophages to the anode, which is reversed to the cathode upon Salmonella infection. This infection-dependent directional switch is independent of the Salmonella pathogenicity island 1 (SPI-1) type III secretion system. The switch is accompanied by a reduction of sialic acids on glycosylated surface components during phagocytosis of bacteria, which is absent in macrophages challenged by microspheres. Moreover, enzymatic cleavage of terminally exposed sialic acids reduces macrophage surface negativity and severely impairs directional migration of macrophages in response to an EF. Based on these findings, we propose that macrophages are attracted to the site of infection by a combination of chemotaxis and galvanotaxis; after phagocytosis of bacteria, surface electrical properties of the macrophage change, and galvanotaxis directs the cells away from the site of infection

Variability of adjacency effects in sky reflectance measurements

Sky reflectance Rsky(l) is used to correct in situ reflectance measurements in the remote detection of water colour. We analysed the directional and spectral variability in Rsky(l) due to adjacency effects against an atmospheric radiance model. The analysis is based on one year of semi-continuous Rsky(l) observations that were recorded in two azimuth directions. Adjacency effects contributed to Rsky(l) dependent on season and viewing angle, and predominantly in the near-infrared (NIR). For our test area, adjacency effects spectrally resembled a generic vegetation spectrum. The adjacency effect was weakly dependent on the magnitude of Rayleigh- and aerosol-scattered radiance. Reflectance differed between viewing directions 5.4 +/- 6.3% for adjacency effects and 21.0 +/- 19.8% for Rayleigh- and aerosol-scattered Rsky(l), in the NIR. It is discussed under which conditions in situ water reflectance observations require dedicated correction for adjacency effects. We provide an open source implementation of our method to aid identification of such conditions. Copyright 2017 Optical Society of America

Validation of a spectral correction procedure for sun and sky reflections in above-water reflectance measurements

A three-component reflectance model (3C) is applied to above-water radiometric measurements to derive remote-sensing reflectance Rrs (λ). 3C provides a spectrally resolved offset Δ(λ) to correct for residual sun and sky radiance (Rayleigh- and aerosol-scattered) reflections on the water surface that were not represented by sky radiance measurements. 3C is validated with a data set of matching above- and below-water radiometric measurements collected in the Baltic Sea, and compared against a scalar offset correction Δ. Correction with Δ(λ) instead of Δ consistently reduced the (mean normalized root-mean-square) deviation between Rrs (λ) and reference reflectances to comparable levels for clear (Δ: 14.3 ± 2.5 %, Δ(λ): 8.2 ± 1.7 %), partly clouded (Δ: 15.4 ± 2.1 %, Δ(λ): 6.5 ± 1.4 %), and completely overcast (Δ: 10.8 ± 1.7 %, Δ(λ): 6.3 ± 1.8 %) sky conditions. The improvement was most pronounced under inhomogeneous sky conditions when measurements of sky radiance tend to be less representative of surface-reflected radiance. Accounting for both sun glint and sky reflections also relaxes constraints on measurement geometry, which was demonstrated based on a semi-continuous daytime data set recorded in a eutrophic freshwater lake in the Netherlands. Rrs (λ) that were derived throughout the day varied spectrally by less than 2 % relative standard deviation. Implications on measurement protocols are discussed. An open source software library for processing reflectance measurements was developed and is made publicly available

Persistence of Supplemented Bifidobacterium longum subsp. infantis EVC001 in Breastfed Infants.

Attempts to alter intestinal dysbiosis via administration of probiotics have consistently shown that colonization with the administered microbes is transient. This study sought to determine whether provision of an initial course of Bifidobacterium longum subsp. infantis (B. infantis) would lead to persistent colonization of the probiotic organism in breastfed infants. Mothers intending to breastfeed were recruited and provided with lactation support. One group of mothers fed B. infantis EVC001 to their infants from day 7 to day 28 of life (n = 34), and the second group did not administer any probiotic (n = 32). Fecal samples were collected during the first 60 postnatal days in both groups. Fecal samples were assessed by 16S rRNA gene sequencing, quantitative PCR, mass spectrometry, and endotoxin measurement. B. infantis-fed infants had significantly higher populations of fecal Bifidobacteriaceae, in particular B. infantis, while EVC001 was fed, and this difference persisted more than 30 days after EVC001 supplementation ceased. Fecal milk oligosaccharides were significantly lower in B. infantis EVC001-fed infants, demonstrating higher consumption of human milk oligosaccharides by B. infantis EVC001. Concentrations of acetate and lactate were significantly higher and fecal pH was significantly lower in infants fed EVC001, demonstrating alterations in intestinal fermentation. Infants colonized by Bifidobacteriaceae at high levels had 4-fold-lower fecal endotoxin levels, consistent with observed lower levels of Gram-negative Proteobacteria and Bacteroidetes. IMPORTANCE The gut microbiome in early life plays an important role for long-term health and is shaped in large part by diet. Probiotics may contribute to improvements in health, but they have not been shown to alter the community composition of the gut microbiome. Here, we found that breastfed infants could be stably colonized at high levels by provision of B. infantis EVC001, with significant changes to the overall microbiome composition persisting more than a month later, whether the infants were born vaginally or by caesarean section. This observation is consistent with previous studies demonstrating the capacity of this subspecies to utilize human milk glycans as a nutrient and underscores the importance of pairing a probiotic organism with a specific substrate. Colonization by B. infantis EVC001 resulted in significant changes to fecal microbiome composition and was associated with improvements in fecal biochemistry. The combination of human milk and an infant-associated Bifidobacterium sp. shows, for the first time, that durable changes to the human gut microbiome are possible and are associated with improved gut function

Development of activity-based language learning of Chinese for a primary school in western Sydney : a participatory action research study

This study explores the use of activity-based learning in the Chinese as a Foreign Language classroom, for young learners in the western Sydney region. The research takes account of current opportunities and the challenges arising from language policy in the Australian context. Activity-based learning is the focus of this research, with hands-on experiments and various classroom activities used rather than having students passively listening to the teacher. In this study, to engage students and to improve their Chinese learning, a wide range of activities, using music, drama games and visual arts, were implemented in the classroom. This thesis aims to answer the following three research questions: How can activity-based learning be used to teach Chinese language to learners who do not have a Chinese language background, in a western Sydney primary school? What factors influence the implementation of an activity-based approach of this kind? What evidence of learning, in relation to outcomes listed in the NSW Chinese K-10 syllabus for Stage 2 students, can be ascertained following the implementation of an activity- based approach to learning Chinese language? This study applied a qualitative methodology using action research with a teacher-as-researcher. The action research involved planning, acting, observing, reflecting and re-planning in a spiral cycle. The teacher-researcher, also a beginning teacher, fostered her own professional learning through reflection, on a weekly basis, on her own teaching practice and used the action research mode to improve the teaching activities. Two cycles of data were collected from a variety of sources, including: feedback from the classroom teacher obtained through observation and interviews; the teacher-researcher’s weekly reflective journal: student focus groups; and checklists completed by students at the end of each cycle. This research shows that the use of an activity-based learning approach for young learners in western Sydney has a significant impact on their Chinese language learning. Such approaches can engage students emotionally, behaviourally and cognitively through a variety of classroom activities. By using various activities such as simple drama games, Chinese songs and visual arts activities, students have achieved the outcomes outlined in the Chinese syllabus, in a relaxing and productive learning environment. Different classroom activities, incorporating various art forms, make Chinese easier to learn and remember, which helps to keep students emotionally engaged and to maintain their interest in learning Chinese. Hence, it is an effective way to motivate students to continue studying Chinese at the next stage. In the meantime, the teacher-researcher’ s professional learning has been enhanced in the process of conducting this research with the help of the teacher-as-researcher mode of action research