Meta-analysis of honey bee neurogenomic response links deformed wing virus type A to precocious behavioral maturation

Crop pollination by the western honey bee Apis mellifera is vital to agriculture but threatened by alarmingly high levels of colony mortality, especially in Europe and North America. Colony loss is due, in part, to the high viral loads of Deformed wing virus (DWV), transmitted by the ectoparasitic mite Varroa destructor, especially throughout the overwintering period of a honey bee colony. Covert DWV infection is commonplace and has been causally linked to precocious foraging, which itself has been linked to colony loss. Taking advantage of four brain transcriptome studies that unexpectedly revealed evidence of covert DWV-A infection, we set out to explore whether this effect is due to DWV-A mimicking naturally occurring changes in brain gene expression that are associated with behavioral maturation. Consistent with this hypothesis, we found that brain gene expression profiles of DWV-A infected bees resembled those of foragers, even in individuals that were much younger than typical foragers. In addition, brain transcriptional regulatory network analysis revealed a positive association between DWV-A infection and transcription factors previously associated with honey bee foraging behavior. Surprisingly, single-cell RNA-Sequencing implicated glia, not neurons, in this effect; there are relatively few glial cells in the insect brain and they are rarely associated with behavioral plasticity. Covert DWV-A infection also has been linked to impaired learning, which together with precocious foraging can lead to increased occurrence of infected bees from one colony mistakenly entering another colony, especially under crowded modern apiary conditions. These findings provide new insights into the mechanisms by which DWV-A affects honey bee health and colony survival

Angular clustering of galaxies at 3.6 microns from the Spitzer Wide-area Infrared Extragalactic (SWIRE) Survey

We present the first analysis of large-scale clustering from the Spitzer Wide-area Infrared Extragalactic legacy survey (SWIRE). We compute the angular correlation function of galaxies selected to have 3.6 m fluxes brighter than 32 Jy in three fields totaling 2 deg2 in area. In each field we detect clustering with a high level of significance. The amplitude and slope of the correlation function is consistent between the three fields and is modeled as w() ¼ A1 with A ¼ (0:6 0:3) ; 10 3; ¼ 2:03 0:10. With a fixed slope of ¼ 1:8, we obtain an amplitude of A ¼ (1:7 0:1) ; 10 3. Assuming an equivalent depth of K 18:7 mag we find that our errors are smaller but our results are consistent with existing clustering measurements in K-band surveys and with stable clustering models. We estimate our median redshift z ’ 0:75, and this allows us to obtain an estimate of the three-dimensional correlation function (r), for which we find r0 ¼ 4:4 0:1 h 1 Mpc

Reference Transcriptome for a Facultatively Eusocial Bee, Megalopta genalis

This article analyses gendering processes in two distinct models of work organization. It is a widespread belief that, compared to hierarchical (Tayloristic) organizations, team-based work offers opportunities for a high quality of working life to a broader range of employees, both men and women. Our research, however, suggests that gender inequality is (re)produced in both settings and results from the so-called gender subtext. The gender subtext is the set of often concealed power-based processes (re)producing gender distinction in social practices through organizational and individual arrangements. We draw a comparison between the gender subtext of Tayloristic and team-based work organizations through a theoretical analysis, illustrated by empirical data concerning the functioning of the gender subtext in organizations in the Dutch banking sector. Taylorism and team-based work differ in their conceptualization of organization and job design, but, when it comes to the gender subtext, it is six of one and half a dozen of the other. We argue that in both approaches a gender subtext contributes to the emergence of different but gendered notions of the ‘disembodied worker’. In both cases the notion of the abstract worker is implicitly loaded with masculine connotations. This gender bias is supported by two factors influencing the gendering of jobs: the gender connotations of care responsibilities and of qualification profiles. These implicit connotations produce and reinforce unequal opportunities for men and women to get highly qualified or management jobs. Our research, therefore, questions the self-evidence of stating that team-based work will offer opportunities for a higher quality of working life for women

Determining the Causal Link of Honey Bee Gut Microbial Composition on Behavioral Maturation

Emerging studies have supported the association between gut microbiome and host behaviors. However, it is unclear whether changes in the gut microbiome cause changes in host behaviors or vice versa. The European honey bee, Apis mellifera, is an excellent animal model for identifying the causal link between microbiome and behavioral changes over the lifetime of the host as the honey bee gut contains a simple microbiome composed of only nine bacterial taxa clusters. In honey bees, division of labor occurs through behavioral maturation where age determines what task a bee does. For example, older bees forage while younger bees perform brood care (nursing) and other in-hive tasks. Single cohort colonies (SCCs), or colonies composed of individuals of the same age, uncouple chronological age effects on honey bee behavioral maturation (nursing → foraging). SCCs results from our previous experiment reveal a highly significant difference in the gut microbiota between nurses and foragers, independent of age, specifically in the abundance of Lactobacillus mellis and Bifidobacterium asteroides

Intronic Non-CG DNA hydroxymethylation and alternative mRNA splicing in honey bees

Abstract Background Previous whole-genome shotgun bisulfite sequencing experiments showed that DNA cytosine methylation in the honey bee (Apis mellifera) is almost exclusively at CG dinucleotides in exons. However, the most commonly used method, bisulfite sequencing, cannot distinguish 5-methylcytosine from 5-hydroxymethylcytosine, an oxidized form of 5-methylcytosine that is catalyzed by the TET family of dioxygenases. Furthermore, some analysis software programs under-represent non-CG DNA methylation and hydryoxymethylation for a variety of reasons. Therefore, we used an unbiased analysis of bisulfite sequencing data combined with molecular and bioinformatics approaches to distinguish 5-methylcytosine from 5-hydroxymethylcytosine. By doing this, we have performed the first whole genome analyses of DNA modifications at non-CG sites in honey bees and correlated the effects of these DNA modifications on gene expression and alternative mRNA splicing. Results We confirmed, using unbiased analyses of whole-genome shotgun bisulfite sequencing (BS-seq) data, with both new data and published data, the previous finding that CG DNA methylation is enriched in exons in honey bees. However, we also found evidence that cytosine methylation and hydroxymethylation at non-CG sites is enriched in introns. Using antibodies against 5-hydroxmethylcytosine, we confirmed that DNA hydroxymethylation at non-CG sites is enriched in introns. Additionally, using a new technique, Pvu-seq (which employs the enzyme PvuRts1l to digest DNA at 5-hydroxymethylcytosine sites followed by next-generation DNA sequencing), we further confirmed that hydroxymethylation is enriched in introns at non-CG sites. Conclusions Cytosine hydroxymethylation at non-CG sites might have more functional significance than previously appreciated, and in honey bees these modifications might be related to the regulation of alternative mRNA splicing by defining the locations of the introns

The Effect of Imidacloprid on Honey Bee Queen Fecundity

Imidacloprid is a neonicotinoid insecticide commonly used in agricultural settings to control insect pests by acting as an agonist of acetylcholine receptors and inducing paralysis and mortality. In small doses, imidacloprid can cause loss of memory and foraging ability along with impaired learning and a lowered immune response in western honey bees (Apis mellifera). Effects of neonicotinoid insecticides on colony reproduction have been documented including decreased colony expansion, queen failure and replacement, and decreased queen egg laying. For this study, we examined the effects of imidacloprid on the fecundity of queen bees when their worker attendants were exposed to low doses of imidacloprid through their food source using a novel, labbased, Queen Monitoring Cage (QMC) system. Our results will help elucidate the effect of imidacloprid on the egg laying behaviors of honey bee queens. By comparing the results generated using QMCs to previous studies using full-sized colonies, we will attempt to validate the use of QMCs as a risk assessment tool