Dokumentarische Interpretation von Kinderzeichnungen: Kinder malen ihre KiTa

Die für diesen Beitrag ausgewählten Kinderzeichnungen von 4- bis 6-jährigen Kindern sind im Rahmen des Forschungs- und Praxisentwicklungsprojekts „Kinder als Akteure der Qualitätsentwicklung in KiTas“ entstanden. Die für das Projekt entwickelte Methode „Kinder malen ihre Kita“ wird mit folgendem Stimulus eingeleitet: „Ich möchte/wir möchten, dass ihr mir/uns mal unsere KiTa malt. Ihr könnt sie so malen, wie ihr denkt! Und ihr könnt mir/uns entweder während ihr malt, etwas zu eurem Bild erzählen oder auch danach, wenn es fertig ist“. Sowohl die Kinderzeichnungen als auch die Gespräche mit den Kindern werden einer Dokumentarischen Interpretation unterzogen. In dem Beitrag werden zum einen anhand von zwei Bildern die Arbeitsschritte der dokumentarischen Bildinterpretation und ihre Spezifität in Bezug auf Kinderzeichnungen detailliert vorgeführt. Zum anderen werden die beiden weiteren Bilder sowie die Gesprächssequenzen auf der Ebene der Komparation bzw. der Relationierung verschiedener Datensorten hinzugezogen. Der Beitrag fokussiert methodologische und methodische Fragen, arbeitet aber auch erste homologe Muster heraus, wie Kinder im Alter von vier bis sechs Jahren ihre KiTa in Bild und Text (re-) konstruieren und mit ihren Erfahrungen und Relevanzen ‚aufladen‘

Editorial: Die Dokumentarische Methode in der kindheitspädagogischen Forschung

Das Editorial stellt die Kontur und die Beiträge des Themenhefts zu den dokumentarischen Methoden des Fallarchiv Kindheitspädagogische Forschung vor

Pressure reconstruction from Lagrangian particle tracking with FFT integration

Volumetric time-resolved pressure gradient fields in unsteady flows can be estimated through flow measurements of the material acceleration in the fluid and the assumption of the governing momentum equation. In order to derive pressure, almost exclusively two numerical methods have been used to spatially integrate the pressure gradient until now: first, direct path integration in the spatial domain, and second, the solution of the Poisson equation with numerical methods. We propose an alternative method by integrating the pressure gradient field directly in Fourier space with a standard FFT function. The method is fast and easy to implement. We demonstrate the accuracy of the integration scheme on a synthetic pressure field and apply it to an experimental example based on acceleration data from Lagrangian particle tracking with high seeding density (Shake-The-Box method)

Large-scale volumetric flow measurement in a pure thermal plume by dense tracking of helium-filled soap bubbles

We present a spatially and temporally highly resolved flow measurement covering a !arge volume (~o.6 m3) in a pure thermal plume in air. The thermal plume develops above an extended heat source and is characterized by moderate velocities (U~0.35 m/s) with a Reynolds number of Re~500 and a Rayleigh number of Ra~100000. We demonstrate the requirements and capa bilities of the measurement equipment and the particle tracking approach to be able to probe measurement volumes up to and beyond one cubic meter. The use of !arge tracer particles (300 µm), helium-filled soap bubbles (HFSBs), is crucial and yields high particle image quality over large-volume depths when illuminated with arrays of pulsed high-power LEDs. The experimental limitations of the HFSBs-their limited lifetime and their intensity loss over time-are quantified. The HFSBs' uniform particle images allows an accurate reconstruction of the flow using Shake-The-Box particle tracking with high partlcle concentrations up to 0.1 particles per pixel. This enables tracking of up to 275,000 HFSBs simultaneously. After interpolating the scattered data onto a regular grid with a Navier-Stokes regularization, the velocity field of the thermal plume reveals a multitude of vortices with a smooth temporal evolution and a remarkable coherence in time (see animation, supplementary data). Acceleration fields are also derived from interpolated particle tracks and complement the flow measurement. Additionally, the flow map, the basis of a !arge dass of Lagrangian coherent structures, is computed directly from observed particle tracks. We show entrainment regions and coherent vortices of the thermal plume in the flow map and compute fields of the finite-time Lyapunov exponent

Loss-of-function of the ciliopathy protein Cc2d2a disorganizes the vesicle fusion machinery at the periciliary membrane and indirectly affects Rab8-trafficking in zebrafish photoreceptors

Ciliopathies are human disorders caused by dysfunction of primary cilia, ubiquitous organelles involved in transduction of environmental signals such as light sensation in photoreceptors. Concentration of signal detection proteins such as opsins in the ciliary membrane is achieved by RabGTPase-regulated polarized vesicle trafficking and by a selective barrier at the ciliary base, the transition zone (TZ). Dysfunction of the TZ protein CC2D2A causes Joubert/Meckel syndromes in humans and loss of ciliary protein localization in animal models, including opsins in retinal photoreceptors. The link between the TZ and upstream vesicle trafficking has been little explored to date. Moreover, the role of the small GTPase Rab8 in opsin-carrier vesicle (OCV) trafficking has been recently questioned in a mouse model. Using correlative light and electron microscopy and live imaging in zebrafish photoreceptors, we provide the first live characterization of Rab8-mediated trafficking in photoreceptors in vivo. Our results support a possibly redundant role for both Rab8a/b paralogs in OCV trafficking, based on co-localization of Rab8 and opsins in vesicular structures, and joint movement of Rab8-tagged particles with opsin. We further investigate the role of the TZ protein Cc2d2a in Rab8-mediated trafficking using cc2d2a zebrafish mutants and identify a requirement for Cc2d2a in the latest step of OCV trafficking, namely vesicle fusion. Progressive accumulation of opsin-containing vesicles in the apical portion of photoreceptors lacking Cc2d2a is caused by disorganization of the vesicle fusion machinery at the periciliary membrane with mislocalization and loss of the t-SNAREs SNAP25 and Syntaxin3 and of the exocyst component Exoc4. We further observe secondary defects on upstream Rab8-trafficking with cytoplasmic accumulation of Rab8. Taken together, our results support participation of Rab8 in OCV trafficking and identify a novel role for the TZ protein Cc2d2a in fusion of incoming ciliary-directed vesicles, through organization of the vesicle fusion machinery at the periciliary membrane

Agrin mediates chondrocyte homeostasis and requires both LRP4 and alpha-dystroglycan to enhance cartilage formation in vitro and in vivo

Objectives Osteoarthritis (OA) is a leading cause of disability for which there is no cure. The identification of molecules supporting cartilage homeostasis and regeneration is therefore a major pursuit in musculoskeletal medicine. Agrin is a heparan sulfate proteoglycan which, through binding to low-density lipoprotein receptor-related protein 4 (LRP4), is required for neuromuscular synapse formation. In other tissues, it connects the cytoskeleton to the basement membrane through binding to α-dystroglycan. Prompted by an unexpected expression pattern, we investigated the role and receptor usage of agrin in cartilage. Methods Agrin expression pattern was investigated in human osteoarthritic cartilage and following destabilisation of the medial meniscus in mice. Extracellular matrix (ECM) formation and chondrocyte differentiation was studied in gain and loss of function experiments in vitro in three-dimensional cultures and gain of function in vivo, using an ectopic cartilage formation assay in nude mice. Receptor usage was investigated by disrupting LRP4 and α-dystroglycan by siRNA and blocking antibodies respectively. Results Agrin was detected in normal cartilage but was progressively lost in OA. In vitro, agrin knockdown resulted in reduced glycosaminoglycan content, downregulation of the cartilage transcription factor SOX9 and other cartilage-specific ECM molecules. Conversely, exogenous agrin supported cartilage differentiation in vitro and ectopic cartilage formation in vivo. In the context of cartilage differentiation, agrin used an unusual receptor repertoire requiring both LRP4 and α-dystroglycan. Conclusions We have discovered that agrin strongly promotes chondrocyte differentiation and cartilage formation in vivo. Our results identify agrin as a novel potent anabolic growth factor with strong therapeutic potential in cartilage regeneration

Enforcing Temporal Consistency in Physically Constrained Flow Field Reconstruction with FlowFit by Use of Virtual Tracer Particles

Processing techniques for particle based optical flow measurement data such as 3D Particle Tracking Velocimetry (PTV) or the novel dense Lagrangian Particle Tracking method Shake-The-Box (STB) can provide time-series of velocity and acceleration information scattered in space. The following post-processing is key to the quality of space-filling velocity and pressure field reconstruction from the scattered particle data. In this work we describe a straight-forward extension of the recently developed data assimilation scheme FlowFit, which applies physical constraints from the Navier-Stokes equations in order to simultaneously determine velocity and pressure fields as solutions to an inverse problem. We propose the use of additional artificial Lagrangian tracers (virtual particles), which are advected between the flow fields at single time instants to achieve meaningful temporal coupling. This is the most natural way of a temporal constraint in the Lagrangian data framework. Not FlowFit's core method is altered in the current work, but its input in form of Lagrangian tracks. This work shows that the introduction of such particle memory to the reconstruction process significantly improves the resulting flow fields. The method is validated in virtual experiments with two independent DNS test cases. Several contributions are revised to explain the improvements, including correlations of velocity and acceleration errors in the reconstructions and the flow field regularization within the inverse problem

Evolution of visual guanylyl cyclases and their activating proteins with respect to clade and species-specific visual system adaptation

Membrane guanylyl cyclase receptors are important regulators of local cGMP production, critically influencing cell growth and differentiation as well as ion transport, blood pressure and calcium feedback of vertebrate phototransduction. Currently, seven different subtypes of membrane guanylyl cyclase receptors have been characterized. These receptors have tissue specific expression and are activated either by small extracellular ligands, changing CO$_{2}$ concentrations or, in the case of visual guanylyl cyclases, intracellularly interacting Ca$^{2+}$-dependent activating proteins. In this report, we focus on the visual guanylyl cyclase receptors (GCs) GC-E (gucy2d/e) and GC-F (gucy2f) and their activating proteins (GCAP1/2/3; guca1a/b/c). While gucy2d/e has been detected in all analyzed vertebrates, GC-F receptors are missing in several clades (reptiles, birds, and marsupials) and/or individual species. Interestingly, the absence of GC-F in highly visual sauropsida species with up to 4 different cone-opsins is compensated by an increased number of guanylyl cyclase activating proteins, whereas in nocturnal or visually impaired species with reduced spectral sensitivity it is consolidated by the parallel inactivation of these activators. In mammals, the presence of GC-E and GC-F is accompanied by the expression of one to three GCAPs, whereas in lizards and birds, up to five different GCAPs are regulating the activity of the single GC-E visual membrane receptor. In several nearly blind species, a single GC-E enzyme is often accompanied by a single variant of GCAP, suggesting that one cyclase and one activating protein are both sufficient and required for conferring the basic detection of light

Selective Gene Loss of Visual and Olfactory Guanylyl Cyclase Genes Following the Two Rounds of Vertebrate-Specific Whole-Genome Duplications

Photoreceptors convey visual information and come in two flavors; dim-light and bright-light dedicated rod and cones. Both cell types feature highly specialized phototransduction cascades that convert photonic energy into intracellular signals. Although a substantial amount of phototransduction gene ohnologs are expressed either in rods or cones, visual guanylyl cyclases (GCs) involved in the calcium (Ca2+) dependent feedback regulation of phototransduction are neither rod nor cone specific. The co-existence of visual GCs in both photoreceptor types suggests that specialization of these ohnologs occurred despite their overlapping expression. Here, we analyze gene retention and inactivation patterns of vertebrate visual and closely related olfactory GCs following two rounds (2R) of vertebrate-specific whole-genome duplication events (2R WGD). Although eutherians generally use two visual and one olfactory GC, independent inactivation occurred in some lineages. Sauropsids (birds, lizards, snakes, turtles, and crocodiles) generally have only one visual GC (GC-E). Additionally, turtles (testodes) also lost the olfactory GC (GC-D). Pseudogenization in mammals occurred in specific species/families likely according to functional needs (i.e., many species with reduced vision only have GC-E). Likewise, some species not relying on scent marks lack GC-D, the olfactory GC enzyme. Interestingly, in the case of fish, no species can be found with fewer than three (two visual and one olfactory) genes and the teleost-specific 3R WGD can increase this number to up to five. This suggests that vision in fish now requires at least two visual GCs