Global Dynamical Evolution of the ISM in Star Forming Galaxies - I. High Resolution 3D HD and MHD Simulations: Effect of the Magnetic Field

In star forming disk galaxies, matter circulation between stars and the interstellar gas, and, in particular the energy input by random and clustered supernova explosions, determine the dynamical and chemical evolution of the ISM, and hence of the galaxy as a whole. Using a 3D MHD code with adaptive mesh refinement developed for this purpose, we have investigated the r\^ole of magnetized matter circulation between the gaseous disk and the surrounding galactic halo. Special emphasis has been put on the effect of the magnetic field with respect to the volume and mass fractions of the different ISM ``phases'', the relative importance of ram, thermal and magnetic pressures, and whether the field can prevent matter transport from the disk into the halo. The simulations were performed on a grid with an area of 1 kpc$^{2}$, centered on the solar circle, extending $\pm 10$ kpc perpendicular to the galactic disk with a resolution as high as 1.25 pc. The simulations were run for a time scale of 400 Myr, sufficiently long to avoid memory effects of the initial setup, and to allow for a global dynamical equilibrium to be reached in case of a constant energy input rate. (...) We find that in general gas transport into the halo in 3D is not prevented by an initial disk parallel magnetic field, but only delayed initially, for as long as it is needed to punch holes into the thick magnetized gas disk. The mean volume filling factor of the hot phase in the disk is similar in HD and MHD (the latter with a total field strength of 4.4 $\mu$G) runs, amounting to $\sim 17-21$ for the Galactic supernova rate.Comment: Paper accepted for publication in A&A (31 Jan); 17 pages with 10 Figures including 6 images and 16 plots (all in low quality jpeg files). For better resolution images contact the author

Effect of pretreatment with immune serum on murine sarcoma virus (Moloney) tumour induction and growth.

Regressor serum from MSV-M-infected mice markedly reduced MSV-M oncogenesis when administered i.p. (0-1 ml/mouse) as much as 30 days before i.m. MSV-M infection in adult BALB/c mice. The regressor serum activity appeared to be directly dependent on the amount of IgG, as shown by: (1) inactivity of sera which have low virus-neutralizing antibody content; (2) high effectiveness only of the IgG serum fraction; (3) inactivity of regressor serum incubated with anti-mouse gamma-globulin serum. The regressor serum activity was specific and could not be ascribed to interferon or interferon-inducing factors, antigen-antibody complexes or free antigen. The activity was not suppressed by sublethal irradiation (380 rad) of recipient mice. These results suggest that the activity of regressor serum administered before MSV-M infection is mediated through sensitization of host cells which are not radiosensitive

Antimicrobial Peptides and Skin: A Paradigm of Translational Medicine

Antimicrobial peptides (AMPs) are small, cationic, amphiphilic peptides with broad-spectrum microbicidal activity against both bacteria and fungi. In mammals, AMPs form the first line of host defense against infections and generally play an important role as effector agents of the innate immune system. The AMP era was born more than 6 decades ago when the first cationic cyclic peptide antibiotics, namely polymyxins and tyrothricin, found their way into clinical use. Due to the good clinical experience in the treatment of, for example, infections of mucus membranes as well as the subsequent understanding of mode of action, AMPs are now considered for treatment of inflammatory skin diseases and for improving healing of infected wounds. Based on the preclinical findings, including pathobiochemistry and molecular medicine, targeted therapy strategies are developed and first results indicate that AMPs influence processes of diseased skin. Importantly, in contrast to other antibiotics, AMPs do not seem to propagate the development of antibiotic-resistant micro-organisms. Therefore, AMPs should be tested in clinical trials for their efficacy and tolerability in inflammatory skin diseases and chronic wounds. Apart from possible fields of application, these peptides appear suited as an example of the paradigm of translational medicine for skin diseases which is today seen as a `two-way road' - from bench to bedside and backwards from bedside to bench. Copyright (c) 2012 S. Karger AG, Base

Similarities and differences in the dolomitization history of two coeval Middle Triassic carbonate platforms, Balaton Highland, Hungary

Dolomitization of platform carbonates is commonly the result of multiphase processes. Documentation of the complex dolomitization history is difficult if completely dolomitized sections are studied. Two Middle Anisian sections representing two coeval carbonate platforms were investigated and compared in the present study. Both sections are made up of meter-scale peritidal–lagoonal cycles with significant pedogenic overprint. One of the sections contains non-dolomitized, partially dolomitized, and completely dolomitized intervals, whereas the other is completely dolomitized. Based on investigations of the partially dolomitized section, penecontemporaneous dolomite formation and/or very early post-depositional dolomitization were identified in various lithofacies types. In shallow subtidal facies, porphyrotopic dolomite was found preferentially in microbial micritic fabrics. Microbially induced dolomite precipitation and/or progressive replacement of carbonate sediments could be interpreted for stromatolites. Cryptocrystalline to very finely crystalline dolomite, probably of pedogenic origin, was encountered in paleosoil horizons. Fabric-destructive dolomite commonly found below these horizons was likely formed via reflux of evaporated seawater. As a result of the different paleogeographic settings of the two platforms, their shallow-burial conditions were significantly different. One of the studied sections was located at the basinward platform margin where pervasive fabric-retentive dolomitization took place in a shallow-burial setting, probably via thermal convection. In contrast, in the area of the other, smaller platform shallow-water carbonates were covered by basinal deposits, preventing fluid circulation and accordingly pervasive shallow-burial dolomitization. In the intermediate to deep burial zone, recrystallization of partially dolomitized limestone and occlusion of newly opened fractures and pores by coarsely crystalline dolomite took place

Head and shoulder posture affect scapular mechanics and muscle activity in overhead tasks

Forward head and rounded shoulder posture (FHRSP) is theorized to contribute to alterations in scapular kinematics and muscle activity leading to the development of shoulder pain. However, reported differences in scapular kinematics and muscle activity in those with forward head and rounded shoulder posture are confounded by the presence of shoulder pain. Therefore, the purpose of this study was to compare scapular kinematics and muscle activity in individuals free from shoulder pain, with and without FHRSP. Eighty volunteers were classified as having FHRSP or ideal posture. Scapular kinematics were collected concurrently with muscle activity from the upper and lower trapezius as well as the serratus anterior muscles during a loaded flexion and overhead reaching task using an electromagnetic tracking system and surface electromyography. Separate mixed model analyses of variance were used to compare three-dimensional scapular kinematics and muscle activity during the ascending phases of both tasks. Individuals with FHRSP displayed significantly greater scapular internal rotation with less serratus anterior activity, during both tasks as well as greater scapular upward rotation, anterior tilting during the flexion task when compared with the ideal posture group. These results provide support for the clinical hypothesis that FHRSP impacts shoulder mechanics independent of shoulder pain

The extremophile Acidithiobacillus ferrooxidans possesses a c-di-GMP signalling pathway that could play a significant role during bioleaching of minerals

Artículo de publicación ISIAims: The primary goal of this study was to characterize the existence of a functional c-di-GMP pathway in the bioleaching bacterium Acidithiobacillus ferrooxidans. Methods and Results: A bioinformatic search revealed that the genome sequence of At. ferrooxidans ATCC 23270 codes for several proteins involved in the c-di-GMP pathway, including diguanylate cyclases (DGC), phosphodiesterases and PilZ effector proteins. Overexpression in Escherichia coli demonstrated that four At. ferrooxidans genes code for proteins containing GGDEF/ EAL domains with functional DGC activity. MS/ MS analysis allowed the identification of c-di-GMP in nucleotide preparations obtained from At. ferrooxidans cells. In addition, c-di-GMP levels in cells grown on the surface of solid energetic substrates such as sulfur prills or pyrite were higher than those measured in ferrous iron planktonic cells. Conclusions: At. ferrooxidans possesses a functional c-di-GMP pathway that could play a key role in At. ferrooxidans biofilm formation during bioleaching processes. Significance and Impact of the Study: This is the first global study about the c-di-GMP pathway in an acidophilic bacterium of great interest for the biomining industry. It opens a new way to explore the regulation of biofilm formation by biomining micro-organisms during the bioleaching process.FONDECYT 1080441 DAAD CONICYT MECESUP UCh 04/0

Comparação de estações climáticas virtuais aplicadas a modelo de simulação da cultura do arroz.

A densidade de estações meteorológicas na região Central do Brasil é baixa. Dessa forma, é necessário o uso de estações climáticas virtuais, dentre as quais destacam-se DailyGridded (DG), com estações virtuais apenas para o Brasil, e Nasa/Power (NP), para o globo terrestre. Este estudo teve por objetivo comparar o desempenho das estações climáticas virtuais com dados de estações climáticas em campo

Caracterização ambiental da região produtora de arroz irrigado tropical no Brasil.

Identificar linhagens promissoras e adaptadas a específicos ambientes é um desafio. Isso demanda enorme esforço dos melhoristas devido à complexidade das interações genótipo x ambiente x manejo, havendo também a necessidade de avaliar muitas linhagens em múltiplos ambientes, bem como identificar seus respectivos ambientes alvos. Para minimizar a complexidade das interações e visando uma melhor compreensão da região produtora de arroz irrigado tropical, uma solução é a identificação de ambientes de populações alvos (TPE - Target Population Environment). Assim, pretende-se determinar as áreas homogêneas por meio da análise de cluster das produtividades simuladas pelo modelo Oryzav3

Disponibilização on-line da análise climática da Embrapa Arroz e Feijão.

Este estudo tem como objetivo disponibilizar na internet as análises climáticas e os respectivos dados diários de uma série histórica para as variáveis precipitação, temperatura máxima, mínima e radiação solar global dos campos experimentais das Fazendas Capivara e Palmital

Acidithiobacillus thiooxidans secretome containing a newly described lipoprotein Licanantase enhances chalcopyrite bioleaching rate

The nature of the mineral–bacteria interphase where electron and mass transfer processes occur is a key element of the bioleaching processes of sulfide minerals. This interphase is composed of proteins, metabolites, and other compounds embedded in extracellular polymeric substances mainly consisting of sugars and lipids (Gehrke et al., Appl Environ Microbiol 64(7):2743–2747, 1998). On this respect, despite Acidithiobacilli—a ubiquitous bacterial genera in bioleaching processes (Rawlings, Microb Cell Fact 4(1):13, 2005)—has long been recognized as secreting bacteria (Jones and Starkey, J Bacteriol 82:788–789, 1961; Schaeffer and Umbreit, J Bacteriol 85:492–493, 1963), few studies have been carried out in order to clarify the nature and the role of the secreted protein component: the secretome. This work characterizes for the first time the sulfur (meta)secretome of Acidithiobacillus thiooxidans strain DSM 17318 in pure and mixed cultures with Acidithiobacillus ferrooxidans DSM 16786, identifying the major component of these secreted fractions as a single lipoprotein named here as Licanantase. Bioleaching assays with the addition of Licanantase-enriched concentrated secretome fractions show that this newly found lipoprotein as an active protein additive exerts an increasing effect on chalcopyrite bioleaching rate