MicroRNA and transcription factor co-regulatory networks and subtype classification of seminoma and non-seminoma in testicular germ cell tumors

Recent studies have revealed that feed-forward loops (FFLs) as regulatory motifs have synergistic roles in cellular systems and their disruption may cause diseases including cancer. FFLs may include two regulators such as transcription factors (TFs) and microRNAs (miRNAs). In this study, we extensively investigated TF and miRNA regulation pairs, their FFLs, and TF-miRNA mediated regulatory networks in two major types of testicular germ cell tumors (TGCT): seminoma (SE) and non-seminoma (NSE). Specifically, we identified differentially expressed mRNA genes and miRNAs in 103 tumors using the transcriptomic data from The Cancer Genome Atlas. Next, we determined significantly correlated TF-gene/miRNA and miRNA-gene/TF pairs with regulation direction. Subsequently, we determined 288 and 664 dysregulated TF-miRNA-gene FFLs in SE and NSE, respectively. By constructing dysregulated FFL networks, we found that many hub nodes (12 out of 30 for SE and 8 out of 32 for NSE) in the top ranked FFLs could predict subtype-classification (Random Forest classifier, average accuracy ≥90%). These hub molecules were validated by an independent dataset. Our network analysis pinpointed several SE-specific dysregulated miRNAs (miR-200c-3p, miR-25-3p, and miR-302a-3p) and genes (EPHA2, JUN, KLF4, PLXDC2, RND3, SPI1, and TIMP3) and NSE-specific dysregulated miRNAs (miR-367-3p, miR-519d-3p, and miR-96-5p) and genes (NR2F1 and NR2F2). This study is the first systematic investigation of TF and miRNA regulation and their co-regulation in two major TGCT subtypes

Brzo otkrivanje uzročnika virusnog proljeva goveda u mlijeku iz spremnika pomoću kombinacije metoda umnožene rekombinazne polimeraze i test-traka za „lateral flow“ analizu

Bovine viral diarrhea virus (BVDV) is one of the most prevalent and economically important pathogens of ruminants, and leads to significant financial losses to the livestock industry worldwide. Development of rapid and accurate diagnostic methods is of great importance for the control and eradication of BVDV infection. The aim of this study was to develop a novel isothermal recombinase polymerase amplification (RPA) method combined with a lateral flow dipstick (LFD), for rapid detection of BVDV. RPA primers and a probe targeting the specific conserved 5′-UTR of BVDV genome were designed. The RPA amplification could be finished at a constant temperature of 38 0000C for 15 min, and the amplification product was easily visualized on a simple LFD within 5 min. The detection limit of this assay was 20 copies per reaction, and there was no cross-reactivity with other bovine infectious viruses, such as infectious bovine rhinotracheitis virus (IBRV), bovine enterovirus (BEV), bovine coronavirus (BcoV), bovine parainfluenza virus type 3 (BPIV-3), bovine ephemeral fever virus (BEFV) and bovine respiratory syncytial virus (BRSV). The assay performance on bulk tank milk was also evaluated, and the sensitivity and accuracy of BVDV LFD RPA was compared with real-time RT-PCR. Of 284 pool or bulk tank milk samples, 51 were found to be positive by RPA assay, whereas 52 were positive by real-time RT-PCR. The coincidence rate between LFD RPA and real-time RT-PCR was 97.54% (277/284).Uzročnik virusnog proljeva goveda (BVDV) jedan je od najčešćih i ekonomski važnih patogena preživača koji uzrokuje znatne financijske gubitke u stočarskoj industriji širom svijeta. Razvoj brzih i točnih dijagnostičkih metoda iznimno je važan za kontrolu i iskorjenjivanje zaraze BVDV-om. Cilj ovog istraživanja bio je razviti novu metodu za brzo otkrivanje BVDV-a baziranu na kombinaciji metoda umnožene rekombinazne polimeraze i test-traka za „lateral flow“ analizu. Oblikovane su početnice i probe za umnažanje rekombinazne polimeraze usmjerene na specifični konzervirani 5’-UTR u genomu BVDV-a. Umnažanje se moglo završiti pri konstantnoj temperaturi od 38 °C tijekom 15 minuta i produkt umnažanja je lako vizualiziran na jednostavnoj test-traci za „lateral flow“ analizu unutar 5 minuta. Test je ograničen na 20 kopija po reakciji, pri čemu nije bilo križne reaktivnosti s drugim goveđim zaraznim virusima kao što su infektivni rinotraheitis virusa goveda (IBRV), goveđi enterovirus (BEV), goveđi koronavirus (BcoV), virus goveđe parainfluence tipa 3 (BPIV-3), virus gljivične ephemeralne groznice (BEFV) i goveđi respiratorni sincicijski virus (BRSV). Učinkovitost kombinacije navedenih metoda istražena je i s obzirom na usporedbu osjetljivosti odnosno točnosti koja se dobiva uporabom RT-PCR metode. Od 284 skupna uzorka mlijeka iz spremnika, kombinacijom metoda umnožene rekombinazne polimeraze i test-traka za „lateral flow“ analizu utvrđen je 51 pozitivan uzorak, a RT-PCR 52 pozitivna uzorka. Stopa podudarnosti između navedenih metoda bila je 97,54 % (277/284)

UniMSE: Towards Unified Multimodal Sentiment Analysis and Emotion Recognition

Multimodal sentiment analysis (MSA) and emotion recognition in conversation (ERC) are key research topics for computers to understand human behaviors. From a psychological perspective, emotions are the expression of affect or feelings during a short period, while sentiments are formed and held for a longer period. However, most existing works study sentiment and emotion separately and do not fully exploit the complementary knowledge behind the two. In this paper, we propose a multimodal sentiment knowledge-sharing framework (UniMSE) that unifies MSA and ERC tasks from features, labels, and models. We perform modality fusion at the syntactic and semantic levels and introduce contrastive learning between modalities and samples to better capture the difference and consistency between sentiments and emotions. Experiments on four public benchmark datasets, MOSI, MOSEI, MELD, and IEMOCAP, demonstrate the effectiveness of the proposed method and achieve consistent improvements compared with state-of-the-art methods.Comment: Accepted to EMNLP 2022 main conferenc

Future Fertility of Patients With No Embryo Transfer in Their First IVF Cycle Attempts

ObjectiveWe aimed to evaluate the future outcomes of patients undergoing their first IVF (in vitro fertilization) attempt with no oocyte retrieved, no normal zygotes formed, or no embryos available for transfer and to identify factors affecting the live birth rate.MethodsPatients who underwent no transplantable embryo in their first IVF cycles but carried out several consecutive cycles between January 2012 to December 2020 were retrospectively enrolled and divided into three groups:group A (no egg retrieval), group B (no normal zygotes formed), and group C (no embryos available to transfer). The patients were also divided into the live birth group and non-live birth group according to whether they got a live baby or not. The clinical data and the cumulative clinical outcomes of groups were compared.Results496 patients met the inclusion criteria and enrolled, with 121 patients with no oocytes retrieved in group A, 138 patients with no normal zygotes formed in group B, and 237 patients with no embryos available to transfer in group C. The age [(34.75(5.82) vs 31.91(5.31), P<0.001; 34.75(5.82) vs 32.25(5.72), P<0.001)] and baseline FSH level [(13.04(8.82) vs 10.52(7.39), P=0.005; 13.04(8.82) vs 9.91(5.95), P<0.001)] of women in group A were significantly higher than those in groups B and C. The stable cumulative live birth rate/patient of three groups achieved 18.18% (after 5 cycles, group A), 28.98% (after 3 cycles, group B) and 20.25% (after 7 cycles, group C). Moreover, the multivariate regression analysis showed that female age and basic FSH were main factors affecting live birth outcome of patients with no embryo transfer in their first IVF cycle attempts.ConclusionsThe future clinical outcome may be better in women with no normal zygotes than those with no oocyte retrieved or no available embryo at their first IVF cycle attempts. The main factors influencing the live birth are age and ovarian reserve

Intracytoplasmic sperm injection (ICSI) versus conventional in vitro fertilisation (IVF) in couples with non-severe male infertility (NSMI-ICSI) : protocol for a multicentre randomised controlled trial

Funding This study was supported by National Key Research and Development Program of China (2016YFC1000201; 2018YFC1002104) and the National Science Foundation of China (81730038). The study funders had no rule in the study design, implementation, analysis, manuscript, preparation or decision to submit this article for publication.Peer reviewedPublisher PD

The impact of sociocultural and psychological stress on the outcome of assisted reproductive technology in remarried families

Objective In China, there is a unique type of second marriage (SM) family where the woman is remarried, and the man is experiencing his first marriage. Additionally, the woman is older than the man. Therefore, these families experience many challenges: psychological, emotional, and societal pressure. Such family is a typical sample for studying sociocultural and psychological stress influencing on outcome of assisted reproductive technology (ART). This study aimed to investigate the impact of social psychological stress on the live birth outcomes AR.Methods In this retrospective cohort, second marriage (SM) families who visited the Second Hospital of Hebei Medical University reproductive center between January 2012 to December 2022 were screened, and 561s marriage families (the SM group) with 5600 first marriage (FM) families (the FM group) were included undergoing their first ART cycles. The primary outcome of this study was the live birth rate (LBR).Results The live birth rate (LBR) of SM group (30.7%) is lower than that of the FM group (43.6%) (p < 0.01). After adjustment by logistic regression, the second marriage group (OR = 1.269, 95%CI 1.031–1.562, p = 0.025) were independent factors associated with the outcome of live birth. After propensity score matching (PSM), the live birth rate of SM group (28.7%) is lower than the FM group (35.9%) (0 = 0.011).Conclusion The SM family experience higher levels of social and psychological pressure, which lead to lower level of LBR than FM family

Dataset for Atomistic simulations on liquid Mg-Sr alloys assisted with deep learning potential

&lt;p&gt;This Dataset is for the paper "Atomistic simulations on liquid Mg-Sr alloys assisted with deep learning potential," including an example script, interatomic potentials, and training data.&lt;/p&gt