6 research outputs found

    Impact of daily consumption of Moringa (Moringa oleifera) dry leaf powder on iron status of Senegalese lactating women

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    A randomized study was conducted to test the efficacy of Moringa powder on iron status and weight gain in women. In an open-labelled randomized trial, 82 moderately anaemic, lactating women, aged 26.7� 6.5 years, received a weekly dose of either 100g of Moringa powder(Moringa group) or 120 mg iron sulphate with 0.5 mg folicacid (Control group). Data from 64 women (33 from Moringa group and 31 from Control group) were analyzed. Baseline parameters, socio-economic, anthropometry, haematology, plasma ferritin, and acute phase proteins were comparable in both groups. Low plasma ferritin (< 12 �g/l) indicating iron deficiency was found in 13 and 14 women from the Moringa and Control groups, respectively. After 3 months oftreatment, mean haemoglobin concentrations significantly increased in both groups (p<0.001) but iron stores were unchanged in the Moringa group while they significantly increased in the Control group indicating that consumption of Moringa leaves failed to restore iron stores in anaemic subjects. A slight improvement was observed in the prevalence of anaemia in both groups but anaemia still persisted due to other reasons than iron deficiency anaemia. None of the groups gained weightduring the 3 months. However, the average weight lost was less important in the Moringa group (-0.8 � 2.1 kg) compared to the control group (-1.2� 2.3 kg) but the difference was not significant (p=0.45).The amount of digestible protein in the powder could suggest that the consumption of Moringa was beneficial to the rural women by preventing weight loss during the rainy season. Micronutrient status improvement of vulnerable people in developing countries like Senegal shouldcombine diet-based strategies through production and consumption of animal derived food, vegetable, fruits and food fortification program. However, Moringa Oleifera is one example of local food that can be used in nutritional intervention program, but its use needs additional rigorous clinical trials to confirm its nutritional benefits.&nbsp

    Polyphasic Identification Of A New Thermotolerant Species Of Lactic Acid Bacteria Isolated From Chicken Faeces

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    peer reviewedTwo thermotolerant and desiccation tolerant lactic acid bacteria (TDLAB) were pointed out from twenty isolated strains from soils and dried chicken faeces. Samples were collected in poultry farms in the vicinity of Dakar, Senegal (West Africa). The two new isolates were called Sp.4 (Sp.4=CWBI-B534=LMG7278) and Sp.20 (Sp.20=CWBI-B545=LMG7279). They are Grampositive, catalase-negative, facultatively anaerobic, non-motile, and non-spore-forming rods. Both produce D/L lactic acid via homofermentative pathway. Growth of the strains occurred between 15°C and 44°C. The optimum temperature for growth was in 30°C-37°C temperature and pH 3-8 range. Desiccation treatment in glycerol showed 30% survival rates. Complex total fatty acid pattern of the strains showed the presence of C14:0, C16:0, C16:1, C18:0, C18:1, C18:2, C18:3. SDS-PAGE of total protein of both strains placed them in L. plantarum group. AFLP analysis showed a phylogenetic proximity of the two strains with L. plantarum stricto sensu species. Specific amplified 16s rDNA restriction analysis (ARDRA) of the 16S rDNA gene, however, showed that these thermotolerant strains were not L. plantarum. ITS sequencing revealed that Sp.20 (LMG 7279) could be classed into Lactobacillus paraplantarum species since the short sequence of ITS showed 95% of similarity with reference species. Polyphasic identification shows that Sp.4, (the type strain is LMG 7278T) represent a new species within the genus Lactobacillus with only 88%+/-1 ITS sequence similarity with reference species. For which the name Lactobacillus aminata sp. nov. is proposed

    Impact Of Daily Consumption Of Moringa ( Moringa Oleifera ) Dry Leaf Powder On Iron Status Of Senegalese Lactating Women

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    A randomized study was conducted to test the efficacy of Moringa powder on iron status and weight gain in women. In an open-labelled randomized trial, 82 moderately anaemic, lactating women, aged 26.7± 6.5 years, received a weekly dose of either 100g of Moringa powder (Moringa group) or 120 mg iron sulphate with 0.5 mg folic acid (Control group). Data from 64 women (33 from Moringa group and 31 from Control group) were analyzed. Baseline parameters, socio-economic, anthropometry, haematology, plasma ferritin, and acute phase proteins were comparable in both groups. Low plasma ferritin (< 12 μg/l) indicating iron deficiency was found in 13 and 14 women from the Moringa and Control groups, respectively. After 3 months of treatment, mean haemoglobin concentrations significantly increased in both groups (p>0.001) but iron stores were unchanged in the Moringa group while they significantly increased in the Control group indicating that consumption of Moringa leaves failed to restore iron stores in anaemic subjects. A slight improvement was observed in the prevalence of anaemia in both groups but anaemia still persisted due to other reasons than iron deficiency anaemia. None of the groups gained weight during the 3 months. However, the average weight lost was less important in the Moringa group (-0.8 ± 2.1 kg) compared to the control group (-1.2± 2.3 kg) but the difference was not significant (p=0.45).The amount of digestible protein in the powder could suggest that the consumption of Moringa was beneficial to the rural women by preventing weight loss during the rainy season. Micronutrient status improvement of vulnerable people in developing countries like Senegal should combine diet-based strategies through production and consumption of animal derived food, vegetable, fruits and food fortification program. However, Moringa oleifera is one example of local food that can be used in nutritional intervention program, but its use needs additional rigorous clinical trials to confirm its nutritional benefits

    Acetobacter senegalensis sp nov., a thermotolerant acetic acid bacterium isolated in Senegal (sub-Saharan Africa) from mango fruit (Mangifera indica L.)

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    peer reviewedA thermotolerant acetic acid bacterium, designated strain CWBI-B418(T), isolated in Senegal from mango fruit (Mangifera indica), was characterized in detail by means of genotypic and phenotypic methods. The novel strain was strictly aerobic and exhibited optimal growth on YGM medium at 35 degrees C. Cells were Gram-negative, motile and coccoid. The strain was assigned to the genus Acetobacter on the basis of 16S rRNA gene sequence analysis. DNA-DNA hybridization experiments with its phylogenetically closest relatives showed that strain CWBI-B418(T) represented a novel Acetobactergenospecies. The DNA G +C content of strain CWBI-B418(T) was 56.0 mol%. Phenotypic characteristics enabling the differentiation of strain CWBI-B418(T) from phylogenetically related Acetobacter species were: production of 2-keto-D-gluconic acid from D-glucose, but not 5-keto-D-gluconic acid, production of catalase but not oxidase, growth on yeast extract with 30% D-glucose, growth with ammonium as sole nitrogen source with ethanol as carbon source, utilization of glycerol and ethanol but not maltose or methanol as carbon sources, and growth in the presence of 10% ethanol. Based on the genotypic and phenotypic data presented, strain CWBI-B418(T) clearly represents a novel Acetobacter species, for which the name Acetobacter senegalensis sp. nov. is proposed. The type strain is CWBI-B418(T) (=LMG 23690(T) = DSM 18889(T))
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