Research on Knowledge Organization of Intangible Cultural Heritage Based on Metadata

Metadata has been analyzed and summarized. Based on Dublin Core metadata, combined with the characteristics and forms of intangible cultural heritage, this article explores the metadata for intangible cultural heritage in knowledge organizations based on relevant resource description standards. The Wuhan woodcarving ship model is presented as an example of national intangible cultural heritage to control the application of metadata in intangible cultural heritage knowledge organizations. New ideas are provided for the digital development of intangible cultural heritage

Changes in gravitational forces induce the modification of Arabidopsis thaliana silique pedicel positioning

The laterals of both shoots and roots often maintain a particular angle with respect to the gravity vector, and this angle can change during organ development and in response to environmental stimuli. However, the cellular and molecular mechanisms of the lateral organ gravitropic response are still poorly understood. Here it is demonstrated that the young siliques of Arabidopsis thalinana plants subjected to 3-D clinostat rotation exhibited automorphogenesis with increased growth angles between pedicels and the main stem. In addition, the 3-D clinostat rotation treatment significantly influenced the development of vascular bundles in the pedicel and caused an enlargement of gap cells at the branch point site together with a decrease in KNAT1 expression. Comparisons performed between normal and empty siliques revealed that only the pedicels of siliques with normally developing seeds could change their growth angle under the 3-D clinostat rotational condition, while the pedicels of the empty siliques lost the ability to respond to the altered gravity environment. These results indicate that the response of siliques to altered gravity depends on the normal development of seeds, and may be mediated by vascular bundle cells in the pedicel and gap cells at branch point sites

A proteomic approach to analyzing responses of Arabidopsis thaliana root cells to different gravitational conditions using an agravitropic mutant, pin2 and its wild type

<p>Abstract</p> <p>Background</p> <p>Root gravitropsim has been proposed to require the coordinated, redistribution of the plant signaling molecule auxin within the root meristem, but the underlying molecular mechanisms are still unknown. PIN proteins are membrane transporters that mediate the efflux of auxin from cells. The PIN2 is important for the basipetal transport of auxin in roots and plays a critical role in the transmission of gravity signals perceived in the root cap to the root elongation zone. The loss of function <it>pin2 </it>mutant exhibits a gravity-insensitive root growth phenotype. By comparing the proteomes of wild type and the <it>pin2 </it>mutant root tips under different gravitational conditions, we hope to identify proteins involved in the gravity-related signal transduction.</p> <p>Results</p> <p>To identify novel proteins involved in the gravity signal transduction pathway we have carried out a comparative proteomic analysis of Arabidopsis <it>pin2 </it>mutant and wild type (WT) roots subjected to different gravitational conditions. These conditions included horizontal (H) and vertical (V) clinorotation, hypergravity (G) and the stationary control (S). Analysis of silver-stained two-dimensional SDS-PAGE gels revealed 28 protein spots that showed significant expression changes in altered gravity (H or G) compared to control roots (V and S). Whereas the majority of these proteins exhibited similar expression patterns in WT and <it>pin2 </it>roots, a significant number displayed different patterns of response between WT and <it>pin2 </it>roots. The latter group included 11 protein spots in the H samples and two protein spots in the G samples that exhibited an altered expression exclusively in WT but not in <it>pin2 </it>roots. One of these proteins was identified as annexin2, which was induced in the root cap columella cells under altered gravitational conditions.</p> <p>Conclusions</p> <p>The most interesting observation in this study is that distinctly different patterns of protein expression were found in WT and <it>pin</it>2 mutant roots subjected to altered gravity conditions. The data also demonstrate that PIN2 mutation not only affects the basipetal transport of auxin to the elongation zone, but also results in an altered expression of proteins in the root columella.</p