THE RELATIONSHIP BETWEEN PRODUCTION OF PROTEIN A, CAPSULE STRUCTURE AND SOME ENZYMES OF STAPHYLOCOCCUS AUREUS ISOLATED FROM INFECTIONS OF HUMAN AND ANIMALS

Thirty-five isolates of Staphylococcus were isolated out of 100 clinical specimens were collected from different infections of human and animals. These isolates were identified as Staphylococcus aureus, 15 of them isolated from human, 15 from cows and only 5 isolates from poultry. Twenty-five (71.4%) isolates of different sources revealed positive results for the detection of protein A, capsule formation and enzyme production of phosphatase, DNase and haemolysin. Seven (20 %) isolates showed negative results for both protein A and capsule as well as for the production of enzymes while only three (8.5%) isolates were not capsulated but able to produce protein A and the enzymes. During the detection of protein A, capsule structure and production of some enzymes, we found that the best value of pH used was the range of 7-7.5. The present study indicated that the presence of protein A and capsule structure is highly associated with the virulence of isolates, and loss of protein A doesn't necessary mean loss of capsule as well. The ability of the isolates to produce their essential enzymes to induct the infection is highly correlated with the ability to produce protein A, without considering the source of the isolates. </jats:p

Study About Pathogenic Bacteria Associated With Bovine Mastitis

Out of 100 raw milk samples collected from one hundred cows infected with mastitis, thirty three isolates of different types of pathogenic bacteria were isolated using different types of selective media. &#x0D; On the basis of traditional bacteriological tests, these isolates were identified as the following: 13 isolates of Staphylococcus aureus, 9 isolates of B-haemolytic Streptococci, 6 isolates of Coliform bacteria, 4 isolates of Corynebacterium pyogenes, and only one isolate Bacillus cereus. &#x0D; In addition to these isolates, a number of non-pathogenic bacteria were recovered during the isolation and they were considered as contaminants of milk such as S. epidermidis, C. bovis and B. subtilus. &#x0D; The total number of living bacteria present in milk samples was measured and it was ranged from (1400 to 1800) bact./ml. &#x0D; The isolates of pathogenic bacteria were tested for their sensitivity to different types of antibiotics and the results were: 100% of these isolates were sensitive to Gentamycin and Tetracycline, 11 (84.6%) isolates of S. aureus, 6(66.2%) isolates of B-hacmolytic Streptococci , 4(66.2%) isolates of Coliform bacteria and 3(75%) &#x0D; es of C. pyogenes were sensitive to Ampicillin, Penicillin, Erythromycin and Cepholexin. While Bacillus cereus isolate was resistant to all these antibiotics. &#x0D; As a result of this work, generally the bacteriological quality of this milk is acceptable due to the low number of viable count of bacteria in &#x0D; milk and the isolates showed high level of sensitivity to the antibiotics used in this study.</jats:p

Study About Pathogenic Bacteria Associated With Bovine

Out of 100 raw milk samples collected from one hundred cows infected with mastitis, thirty three isolates of different types of pathogenic bacteria were isolated using different types of selective media. &#x0D; On the basis of traditional bacteriological tests, these isolates were identified as the following: 13 isolates of Staphylococcus aureus, 9 isolates of B-haemolytic Streptococci, 6 isolates of Coliform bacteria, 4 isolates of Corynebacterium pyogenes, and only one isolate Bacillus cereus. &#x0D; In addition to these isolates, a number of non-pathogenic bacteria were recovered during the isolation and they were considered as contaminants of milk such as S. epidermidis, C. bovis and B. subtilus. &#x0D; The total number of living bacteria present in milk samples was measured and it was ranged from (1400 to 1800) bact./ml. &#x0D; The isolates of pathogenic bacteria were tested for their sensitivity to different types of antibiotics and the results were: 100% of these isolates were sensitive to Gentamycin and Tetracycline, 11 (84.6%) isolates of S. aureus, 6(66.2%) isolates of B-hacmolytic Streptococci , 4(66.2%) isolates of Coliform bacteria and 3(75%) &#x0D; es of C. pyogenes were sensitive to Ampicillin, Penicillin, Erythromycin and Cepholexin. While Bacillus cereus isolate was resistant to all these antibiotics. &#x0D; As a result of this work, generally the bacteriological quality of this milk is acceptable due to the low number of viable count of bacteria in &#x0D;  milk and the isolates showed high level of sensitivity to the antibiotics used in this study. </jats:p

Determination of Outdoor Radon Concentrations at Madenat Al-Elem University College using CR-39 detector

In the present work , radon gas concentration ,effectiveradium contentnbsp and both area (surface ) and mass exhalation rates in soil samples collected from different locations in Madenat Al-Elem have been measured using CR-39 track detectors . Values of the average radon gas concentration were found to vary from (25.370 Bq /m3 ) to ( 40.580 Bq / m3 ) . Radium equivalent content values were found to vary from ( 10.186 Bq / kg ) to ( 16.293 Bq / kg ).Area ( surface ) exhalation rate values were found to vary from ( 0.439 Bq/ m h ) to ( 0.700 Bq/m h) . Mass exhalation rate values were found to vary from ( 0.017Bq/ kg h ) to ( 0.027Bq/kg h). All the results obtained in the present work were found to be less than their corresponding world limits .Thus, the present results have revealed that , radon gas concentration , radium equivalent content and both area and mass exhalation rates in the studied area do not pose risk to human health.nbs