Paleo-methane emissions recorded in foraminifera near the landward limit of the gas hydrate stability zone offshore western Svalbard

We present stable isotope and geochemical data from four sediment cores from west of Prins Karls Forland (ca. 340 m water depth), offshore western Svalbard, recovered from close to sites of active methane seepage, as well as from shallower water depths where methane seepage is not presently observed. Our analyses provide insight into the record of methane seepage in an area where ongoing ocean warming may be fueling the destabilization of shallow methane hydrate. The ?13C values of benthic and planktonic foraminifera at the methane seep sites show distinct intervals with negative values (as low as ?27.8‰) that do not coincide with the present-day depth of the sulfate methane transition zone (SMTZ). These intervals are interpreted to record long-term fluctuations in methane release at the present-day landward limit of the gas hydrate stability zone (GHSZ). Shifts in the radiocarbon ages obtained from planktonic foraminifera toward older values are related to methane-derived authigenic carbonate overgrowths of the foraminiferal tests, and prevent us from establishing the chronology of seepage events. At shallower water depths, where seepage is not presently observed, no record of past methane seepage is recorded in foraminifera from sediments spanning the last 14 ka cal BP (14C-AMS dating). ?13C values of foraminiferal carbonate tests appear to be much more sensitive to methane seepage than other sediment parameters. By providing nucleation sites for authigenic carbonate precipitation, foraminifera thus record the position of even a transiently stable SMTZ, which is likely to be a characteristic of temporally variable methane fluxes

Comparative Composition, Diversity and Trophic Ecology of Sediment Macrofauna at Vents, Seeps and Organic Falls

Sediments associated with hydrothermal venting, methane seepage and large organic falls such as whale, wood and plant detritus create deep-sea networks of soft-sediment habitats fueled, at least in part, by the oxidation of reduced chemicals. Biological studies at deep-sea vents, seeps and organic falls have looked at macrofaunal taxa, but there has yet to be a systematic comparison of the community-level attributes of sediment macrobenthos in various reducing ecosystems. Here we review key similarities and differences in the sediment-dwelling assemblages of each system with the goals of (1) generating a predictive framework for the exploration and study of newly identified reducing habitats, and (2) identifying taxa and communities that overlap across ecosystems. We show that deep-sea seep, vent and organic-fall sediments are highly heterogeneous. They sustain different geochemical and microbial processes that are reflected in a complex mosaic of habitats inhabited by a mixture of specialist (heterotrophic and symbiont-associated) and background fauna. Community-level comparisons reveal that vent, seep and organic-fall macrofauna are very distinct in terms of composition at the family level, although they share many dominant taxa among these highly sulphidic habitats. Stress gradients are good predictors of macrofaunal diversity at some sites, but habitat heterogeneity and facilitation often modify community structure. The biogeochemical differences across ecosystems and within habitats result in wide differences in organic utilization (i.e., food sources) and in the prevalence of chemosynthesis-derived nutrition. In the Pacific, vents, seeps and organic-falls exhibit distinct macrofaunal assemblages at broad-scales contributing to ß diversity. This has important implications for the conservation of reducing ecosystems, which face growing threats from human activities

Ice loss from the East Antarctic Ice Sheet during late Pleistocene interglacials

Understanding ice sheet behaviour in the geological past is essential for evaluating the role of the cryosphere in the climate system and for projecting rates and magnitudes of sea level rise in future warming scenarios1,2,3,4. Although both geological data5,6,7 and ice sheet models3,8 indicate that marine-based sectors of the East Antarctic Ice Sheet were unstable during Pliocene warm intervals, the ice sheet dynamics during late Pleistocene interglacial intervals are highly uncertain3,9,10. Here we provide evidence from marine sedimentological and geochemical records for ice margin retreat or thinning in the vicinity of the Wilkes Subglacial Basin of East Antarctica during warm late Pleistocene interglacial intervals. The most extreme changes in sediment provenance, recording changes in the locus of glacial erosion, occurred during marine isotope stages 5, 9, and 11, when Antarctic air temperatures11 were at least two degrees Celsius warmer than pre-industrial temperatures for 2,500 years or more. Hence, our study indicates a close link between extended Antarctic warmth and ice loss from the Wilkes Subglacial Basin, providing ice-proximal data to support a contribution to sea level from a reduced East Antarctic Ice Sheet during warm interglacial intervals. While the behaviour of other regions of the East Antarctic Ice Sheet remains to be assessed, it appears that modest future warming may be sufficient to cause ice loss from the Wilkes Subglacial Basin

Pore Characteristics of Deep-Sea Benthic Foraminifera

A review of the pore patterns of deep-sea benthic foraminifera is presented with a discussion of their characteristics, function and relationship with dissolved oxygen levels. Pore characteristics of deep-sea benthic foraminifera are of timely interest due to their potential for reconstructing dissolved oxygen conditions from the sedimentary record. Scanning electron micrographs of 20 epifaunal and infaunal deep-sea taxa from the Sulu Sea, Monterey Bay, California Bight and northwest Atlantic Ocean are presented to illustrate the wide range of pore patterns found in deep-sea taxa. New SEM observations of three taxa with biconvex test shapes, Oridorsalis umbonatus, Hoeglundina elegans, and Epistominella umbonifera, suggest that these taxa have an infaunal habitat for at least part of their life span

The ecology of living (stained) deep-sea benthic foraminifera from the Sulu Sea

The distribution of living (rose bengal stained) deep-sea benthic foraminifera was determined in the upper 20 cm of sediments of eight Soutar box cores taken from two depth transects (510-4515 m) in the thermospheric (> 10°C) Sulu Sea. Despite the uniformity of bottom water temperatures, salinities, and dissolved oxygen levels below 1000 m, significant faunal differences exist at different depths in the low-oxygen (∼1.25 mL/L below 1000 m) basin. The shallowest site (510 m) is dominated (> 10% of the calcareous fauna) by Cibicidoides, Uvigerina, (> 150 µm) and Bolivina (> 63 µm), while Siphonina is codominant with Cibicidoides and Uvigerina in the 1005-m core. The 2000-m cores are dominated by Cibicidoides, Gyroidinoides, and Oridorsalis, while Cibicidoides bradyi and Oridorsalis umbonatus dominate the 3000- and 4000-m cores. Infaunal assemblages of Valvulineria mexicana are found in the sediments of the 4515-m core. Relatively low bottom water oxygen values do not necessarily yield "typical low-oxygen taxa" such as Bolivina, Uvigerina, Chilostomella, Bulimina, and Globobulimina. Changes in the abundances of these taxa in fossil assemblages have been used as indicators of changes in ancient bottom water oxygen levels but may instead reflect organic carbon contents of the sediments. An examination of the vertical distributions of foraminiferal assemblages from the > 63-µm and > 150-µm fractions reveals that taxa have microhabitat preferences similar to those observed in other regions. Taxa found in the upper 0- to 1-cm interval (epifaunal) include Cibicidoides wuellerstorfi and Hoeglundina elegans, while taxa such as Chilostomella and Globobulimina reach maximum abundances in subsurface sediments and have infaunal microhabitat preferences. Cibicidoides bradyi and O. umbonatus live in sediment depths from 0- to 4-cm and have transitional preferences with both epifaunal and infaunal occurrences. Intrageneric differences in test morphologies, including pore distribution, rounded peripheries, and variable spire height, are observed in Cibicidoides and Gyroidinoides and are suggested to be related to microhabitat preferences. Vertical distributions of a number of taxa found in both the 63- to 150-µm and > 150-µm fractions are similar, suggesting that juveniles and adults live under similar microhabitat conditions. Ontogenetic changes in microhabitat preferences of most species are not observed in this study and therefore would not be expected to account for isotopic vital effects reported for some taxa in previous studies

A New Biological Proxy for Deep-Sea Paleo-Oxygen: Pores of Epifaunal Benthic Foraminifera

The negative consequences of fossil fuel burning for the oceans will likely include warming, acidification and deoxygenation, yet predicting future deoxygenation is difficult. Sensitive proxies for oxygen concentrations in ancient deep-ocean bottom-waters are needed to learn from patterns of marine deoxygenation during global warming conditions in the geological past. Understanding of past oxygenation effects related to climate change will better inform us about future patterns of deoxygenation. Here we describe a new, quantitative biological proxy for determining ocean paleo-oxygen concentrations: the surface area of pores (used for gas exchange) in the tests of deep-sea benthic foraminifera collected alive from 22 locations (water depths: 400 to 4100 m) at oxygen levels ranging from ~ 2 to ~ 277 μmol/l. This new proxy is based on species that are widely distributed geographically, bathymetrically and chronologically, and therefore should have broad applications. Our calibration demonstrates a strong, negative logarithmic correlation between bottom-water oxygen concentrations and pore surface area, indicating that pore surface area of fossil epifaunal benthic foraminifera can be used to reconstruct past changes in deep ocean oxygen and redox levels

Isolation of mitochondrial DNA from cytoplasmic male sterile and maintainer lines of pearl millet, Pennisetum americanum (L.) Leeke

Mitochondrial DNA has been isolated from paired lines of pearl millet maintainer and cytoplasmic male sterile plants. Evaluation of the DNA by agarose gel electrophoresis shows that good quality DNA of high molecular weight can be obtained from mitochondria of both maintainer and male sterile pearl mille

Viral and host cellular transcription in Autographa californica nuclear polyhedrosis virus-infected gypsy moth cell lines

Infection of two gypsy moth cell lines (IPLB-Ld652Y and IPLB-LdFB) by the Autographa californica multiple-enveloped nuclear polyhedrosis virus (AcMNPV) is characterized by extremely attenuated viral protein synthesis followed by a total arrest of all viral and cellular protein production. In this study, AcMNPV- and host cell-specific transcription were examined. Overall levels of viral RNAs in infected gypsy moth cells were, at most measured times, comparable to RNA levels from an infected cell line (TN-368) permissive for AcMNPV replication. Northern blot (RNA) analyses using viral and host gene-specific probes revealed predominantly normal-length virus- and cell-specific transcripts postinfection. Transport of viral RNAs from the nucleus to the cytoplasm and transcript stability in infected gypsy moth cells also appeared normal compared with similar parameters for AcMNPV-infected TN-368 cells. Host cellular and viral mRNAs extracted from gypsy moth and TN-368 cells at various times postinfection and translated in vitro yielded similar spectra of host and viral proteins. Treatment of infected gypsy moth cells with the DNA synthesis inhibitor aphidicolin eliminated the total protein synthesis shutoff in infected IPLB-LdFB cells but had no effect on protein synthesis inhibition in infected IPLB-Ld652Y cells. The apparent selective block in the translation of viral transcripts early in infection and the absence of normal translation or transcription of host cellular genes at later times is discussed.</jats:p