MAPPING AND EXPRESSION OF A REGULATORY NITROGEN-FIXATION GENE (FIXD) OF RHIZOBIUM-MELILOTI

WEBER G, REILANDER H, Pühler A. MAPPING AND EXPRESSION OF A REGULATORY NITROGEN-FIXATION GENE (FIXD) OF RHIZOBIUM-MELILOTI. EMBO JOURNAL. 1985;4(11):2751-2756

RHIZOBIUM-MELILOTI-NIFN (FIXF) GENE IS PART OF AN OPERON REGULATED BY A NIFA-DEPENDENT PROMOTER AND CODES FOR A POLYPEPTIDE HOMOLOGOUS TO THE NIFK GENE-PRODUCT

Aguilar OM, Reilander H, Arnold W, Pühler A. RHIZOBIUM-MELILOTI-NIFN (FIXF) GENE IS PART OF AN OPERON REGULATED BY A NIFA-DEPENDENT PROMOTER AND CODES FOR A POLYPEPTIDE HOMOLOGOUS TO THE NIFK GENE-PRODUCT. JOURNAL OF BACTERIOLOGY. 1987;169(12):5393-5400

Residues within transmembrane helices 2 and 5 of the human gonadotropin-releasing hormone receptor contribute to agonist and antagonist binding

To understand the ligand binding properties of the human GnRH receptor (hGnRH-R), 24 site-specific mutants within transmembrane helices (TMH) 1, 2, and 5 and the extracellular loop 2 (E2) were generated. These mutants were analyzed by using a functional reporter gene assay, monitoring receptor signaling via adenylate cyclase to a cAMP-responsive element fused to Photinus pyralis luciferase. The functional behavior of 14 receptor mutants, capable of G-protein coupling and signaling, was studied in detail with different well described agonistic and antagonistic peptide ligands. Furthermore, the binding constants were determined in displacement binding experiments with the antagonist