The Living Rainforest Sustainable Greenhouses

The Living Rainforest (www.livingrainforest.org) is an educational charity that uses rainforest ecology as a metaphor for communicating general sustainability issues to the public. Its greenhouses and office buildings are to be renovated using the most sustainable methods currently available. This will be realised through construction of a high insulating greenhouse covering with a k-value of less than 2 Wm-2K-1, passive seasonal storage of excess summer solar energy in the ground by a ground source heat exchanger and exploitation of this low grade solar energy for heating in winter by a heat pump. In winter the heat pump will produce cold water to cool the ground allowing a passive cooling function in summer via the GSHE. It will be demonstrated that a GSHE is an alternative for an open aquifer in regions with no aquifer availability. The heat pump will deliver the heating baseload, the peak load will be delivered by a biomass boiler, fired with locally-sourced low-cost wood chips. It is expected that the energy saving will be about 75%, resulting in a major cost reduction. The low k-value of the covering is linked to a light transmission of 75 %. This is high enough for the demands of the vegetation in The Living Rainforest. Because the inner greenhouse climate demands are comparable to that of ornamentals, the results will be applicable to commercial ornamental production. In future low k-value coverings will also be available with high light transmission, allowing wider application of the results. This paper focuses on the correlation between k-value, light transmission and energy demand in order to investigate the trade-off between light transmittance (a major energy gain) and heat loss. The effects of these design parameters on storage and harvesting capacity are also considered but appear to have a low sensitivity. The renovated greenhouse site at The Living Rainforest will show that new greenhouses and ecology can be linked to sustainability and this will be communicated and demonstrated to the public

Correcting the polarization effect in low frequency Dielectric Spectroscopy

We demonstrate a simple and robust methodology for measuring and analyzing the polarization impedance appearing at interface between electrodes and ionic solutions, in the frequency range from 1 to $10^6$ Hz. The method assumes no particular behavior of the electrode polarization impedance and it only makes use of the fact that the polarization effect dies out with frequency. The method allows a direct and un-biased measurement of the polarization impedance, whose behavior with the applied voltages and ionic concentration is methodically investigated. Furthermore, based on the previous findings, we propose a protocol for correcting the polarization effect in low frequency Dielectric Spectroscopy measurements of colloids. This could potentially lead to the quantitative resolution of the $\alpha$-dispersion regime of live cells in suspension

The mPower Study, Parkinson Disease Mobile Data Collected Using Researchkit

Current measures of health and disease are often insensitive, episodic, and subjective. Further, these measures generally are not designed to provide meaningful feedback to individuals. The impact of high-resolution activity data collected from mobile phones is only beginning to be explored. Here we present data from mPower, a clinical observational study about Parkinson disease conducted purely through an iPhone app interface. The study interrogated aspects of this movement disorder through surveys and frequent sensor-based recordings from participants with and without Parkinson disease. Benefitting from large enrollment and repeated measurements on many individuals, these data may help establish baseline variability of real-world activity measurement collected via mobile phones, and ultimately may lead to quantification of the ebbs-and-flows of Parkinson symptoms. App source code for these data collection modules are available through an open source license for use in studies of other conditions. We hope that releasing data contributed by engaged research participants will seed a new community of analysts working collaboratively on understanding mobile health data to advance human health

A New Galactic Extinction Map of the Cygnus Region

We have made a Galactic extinction map of the Cygnus region with 5' spatial resolution. The selected area is 80^\circ to 90^\circ in the Galactic longitude and -4^\circ to 8^\circ in the Galactic latitude. The intensity at 140 \mum is derived from the intensities at 60 and 100 \mum of the IRAS data using the tight correlation between 60, 100, and 140 \mum found in the Galactic plane. The dust temperature and optical depth are calculated with 5' resolution from the 140 and 100 \mum intensity, and Av is calculated from the optical depth. In the selected area, the mean dust temperature is 17 K, the minimum is 16 K, and the maximum is 30 K. The mean Av is 6.5 mag, the minimum is 0.5 mag, and the maximum is 11 mag. The dust temperature distribution shows significant spatial variation on smaller scales down to 5'. Because the present study can trace the 5'-scale spatial variation of the extinction, it has an advantage over the previous studies, such as the one by Schlegel, Finkbeiner, & Davis, who used the COBE/DIRBE data to derive the dust temperature distribution with a spatial resolution of 1^\circ. The difference of Av between our map and Schlegel et al.'s is \pm 3 mag. A new extinction map of the entire sky can be produced by applying the present method.Comment: 27 pages, 14 figures, accepted for publication in Ap

Evaluating 5-nitrofurans as trypanocidal agents

The nitroheterocycle nifurtimox, as part of a nifurtimox-eflornithine combination therapy, represents one of a limited number of treatments targeting Trypanosoma brucei, the causative agent of human African trypanosomiasis. The mode of action of this prodrug involves an initial activation reaction catalysed by a type I nitroreductase (NTR), an enzyme found predominantly in prokaryotes, leading to the formation of a cytotoxic unsaturated open chain nitrile metabolite. Here, we evaluate the trypanocidal activity of a library of other 5-nitrofurans against bloodstream form T. brucei as a preliminary step in the identification of additional nitroaromatic compounds that could potentially partner eflornithine. Biochemical screening against purified enzyme revealed that all 5-nitrofurans were effective substrates for TbNTR with the preferred compounds having apparent kcat/KM values approximately 50-fold greater than nifurtimox. For several compounds, in vitro reduction by this nitroreductase yielded products characterized by mass spectroscopy as either unsaturated or saturated open chain nitriles. When tested against bloodstream form T. brucei, many of the derivatives displayed significant growth inhibitory properties with the most potent compounds generating IC50 values around 200 nM. The anti-parasitic activity of the most potent agents was demonstrated to be NTR dependent as parasites having reduced levels of the enzyme displayed resistance to the compounds while parasites over expressing TbNTR showed hypersensitivity. We conclude that other members of the 5-nitrofurans class of nitroheterocycles have potential to treat human African trypanosomiasis perhaps as an alternative partner prodrug to nifurtimox in the next generation of eflornithine-based combinational therapies

Preliminary Investigation of the Frictional Response of Reptilian Shed Skin

Developing deterministic surfaces relies on controlling the structure of the rubbing interface so that not only the surface is of optimized topography, but also is able to self-adjust its tribological behaviour according to the evolution of sliding conditions. In seeking inspirations for such designs, many engineers are turning toward the biological world to correlate surface structure to functional behavior of bio-analogues. From a tribological point of view, squamate reptiles offer diverse examples where surface texturing, submicron and nano-scale features, achieve frictional regulation. In this paper, we study the frictional response of shed skin obtained from a snake (Python regius). The study employed a specially designed tribo-acoustic probe capable of measuring the coefficient of friction and detecting the acoustical behavior of the skin in vivo. The results confirm the anisotropy of the frictional response of snakes. The coefficient of friction depends on the direction of sliding: the value in forward motion is lower than that in the backward direction. In addition it is shown that the anisotropy of the frictional response may stem from profile asymmetry of the individual fibril structures present within the ventral scales of the reptil

The social cognition of medical knowledge, with special reference to childhood epilepsy

This paper arose out of an engagement in medical communication courses at a Gulf university. It deploys a theoretical framework derived from a (critical) sociocognitive approach to discourse analysis in order to investigate three aspects of medical discourse relating to childhood epilepsy: the cognitive processes that are entailed in relating different types of medical knowledge to their communicative context; the types of medical knowledge that are constituted in the three different text types analysed; and the relationship between these different types of medical knowledge and the discursive features of each text type. The paper argues that there is a cognitive dimension to the human experience of understanding and talking about one specialized from of medical knowledge. It recommends that texts be studied in medical communication courses not just in terms of their discrete formal features but also critically, in terms of the knowledge which they produce, transmit and reproduce

Rescue of Murine F508del CFTR Activity in Native Intestine by Low Temperature and Proteasome Inhibitors

Most patients with Cystic Fibrosis (CF) carry at least one allele with the F508del mutation, resulting in a CFTR chloride channel protein with a processing, gating and stability defect, but with substantial residual activity when correctly sorted to the apical membranes of epithelial cells. New therapies are therefore aimed at improving the folding and trafficking of F508del CFTR, (CFTR correctors) or at enhancing the open probability of the CFTR chloride channel (CFTR potentiators). Preventing premature breakdown of F508del CFTR is an alternative or additional strategy, which is investigated in this study. We established an ex vivo assay for murine F508del CFTR rescue in native intestinal epithelium that can be used as a pre-clinical test for candidate therapeutics. Overnight incubation of muscle stripped ileum in modified William's E medium at low temperature (26°C), and 4 h or 6 h incubation at 37°C with different proteasome inhibitors (PI: ALLN, MG-132, epoxomicin, PS341/bortezomib) resulted in fifty to hundred percent respectively of the wild type CFTR mediated chloride secretion (forskolin induced short-circuit current). The functional rescue was accompanied by enhanced expression of the murine F508del CFTR protein at the apical surface of intestinal crypts and a gain in the amount of complex-glycosylated CFTR (band C) up to 20% of WT levels. Sustained rescue in the presence of brefeldin A shows the involvement of a post-Golgi compartment in murine F508del CFTR degradation, as was shown earlier for its human counterpart. Our data show that proteasome inhibitors are promising candidate compounds for improving rescue of human F508del CFTR function, in combination with available correctors and potentiators