Annexin A2 Coordinates STAT3 to Regulate the Invasion and Migration of Colorectal Cancer Cells In Vitro

The present study aimed to reveal the expression of STAT3 and Anxa 2 in CRC specimens and to investigate the effects of STAT3 and Anxa 2 signaling on the proliferation, invasion, and migration in CRC Caco-2 cells. Results demonstrated that both Anxa 2 and STAT3 were highly expressed in CRC specimens in both mRNA and protein levels, with or without phosphorylation (Tyrosine 23 in Anxa 2 and Tyrosine 705 in STAT3). And the upregulated Anxa 2 promoted the phosphorylation of STAT3 (Tyrosine 705) in CRC Caco-2 cells. The upregulated Anxa 2 promoted the proliferation, migration, and invasion of Caco-2 cells in vitro. Moreover, the STAT3 knockdown also repressed the proliferation, migration, and invasion of Caco-2 cells. In conclusion, the overexpressed Annexin A2 regulated the proliferation, invasion, and migration in CRC cells in an association with STAT3

Remimazolam in pediatric anesthesia: a systematic review for clinical decision-making

BackgroundRemimazolam's role in pediatric anesthesia is evolving. We systematically reviewed 2024–2025 evidence to establish a clinical decision-making framework for its use.MethodsFollowing PRISMA guidelines, a systematic search identified 23 studies (15 RCTs) involving 2,847 pediatric patients for narrative synthesis.ResultsRemimazolam demonstrated superior hemodynamic stability vs. propofol (cardiovascular complications: RR 0.30, 95% CI 0.20–0.46) and reduced emergence delirium by 61% (RR 0.39, 95% CI 0.21–0.70). The CES1 G143E polymorphism was identified as a genetic basis for prolonged sedation, reducing drug clearance >90%. Critical limitations include a 15% re-sedation rate post-flumazenil, a complete lack of data in infants <1 year, and unknown long-term neurodevelopmental safety.ConclusionRemimazolam represents a valuable anesthetic tool with specific advantages in pediatric anesthesia. While it demonstrates superior hemodynamic stability and reduced emergence delirium compared to standard agents, it is not a universal replacement for established anesthetics. Current evidence supports its use in specific clinical scenarios, particularly for preventing post-sevoflurane emergence delirium and in hemodynamically unstable patients. However, the absence of infant and long-term neurodevelopmental safety data necessitates continued research before widespread adoption.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD420251058023, PROSPERO CRD420251058023

Responses of sequential and hierarchical phenological events to warming and cooling in alpine meadows

Organisms' life cycles consist of hierarchical stages, from a single phenological stage (for example, flowering within a season), to vegetative and reproductive phases, to the total lifespan of the individual. Yet phenological events are typically studied in isolation, limiting our understanding of life history responses to climate change. Here, we reciprocally transfer plant communities along an elevation gradient to investigate plastic changes in the duration of sequential phenological events for six alpine species. We show that prolonged flowering leads to longer reproductive phases and activity periods when plants are moved to warmer locations. In contrast, shorter post-fruiting leaf and flowering stages led to shorter vegetative and reproductive phases, respectively, which resulted in shorter activity periods when plants were moved to cooler conditions. Therefore, phenological responses to warming and cooling do not simply mirror one another in the opposite direction, and low temperature may limit reproductive allocation in the alpine region

The Evolution of the Interaction Between Urban Rail Transit and Land Use: A CiteSpace-Based Knowledge Mapping Approach

Urban rail transit is a key enabler for optimizing urban spatial structures, and its interactive relationship with land use has long been a focus of attention. However, existing studies suffer from scattered methodologies, a lack of systematic analysis, and insufficient dynamic insights into global trends. This study comprehensively employs CiteSpace, VOSviewer, and Scimago Graphica to conduct bibliometric and knowledge map analysis on 1894 articles from the Web of Science database between 2004 and 2024, focusing on global research trends, collaboration networks, thematic evolution, and methodological advancements. Key findings include the following: (1) research on rail transit and land use has been steadily increasing, with a significant “US-China dual-core” distribution, where most studies are concentrated in the United States and China, with higher research density in Asia; (2) domestic and international research has primarily focused on themes such as the built environment, value capture, and public transportation, with a recent shift toward artificial intelligence and smart city technology applications; (3) research methods have evolved from foundational 3S technologies (GIS, GPS, RS) to spatial modeling tools (e.g., LUTI model, node-place model), and the current emergence of AI-driven analysis (e.g., machine learning, deep learning, digital twins). The study identifies three future research directions—technology integration, data governance, and institutional innovation—which provide guidance for the coordinated planning of transportation and land use in future smart city development

Can CDM projects trigger host countries’ innovation in renewable energy? Evidence of firm-level dataset from China

The goal of the Clean Development Mechanism (CDM) is both emission reduction and sustainable development, in this process, whether the host country's own technological innovation can be promoted should be an important part of the post assessment of CDM projects. This paper examines whether the CDM projects accumulated at the province level are positively correlated with Chinese firms’ innovation in technological fields associated with renewable energy and energy efficiency. We assemble a unique firm-level dataset with both firms’ patent innovation in renewable energy and CDM projects accounted for. We obtain some novel findings. CDM projects have contributed to the firm-level innovation in renewable energy and energy efficiency. The heterogeneity is detected. This positive enhancement effect is pronounced in invention patents, but is muted in utility patents. The induced innovation effect varies across industrial energy intensity. The more energy intensive the industry, the larger impacts the firms could absorb from the CDM projects. When inspecting CDM by specific energy type, the type-specific induced innovation effect is only documented for biofuel-related CDM projects, indicating that firm-level innovation in biofuels appears to benefit from the related CDM projects within the same province. Lastly, we do find some regional spillover effects in which the CDM projects accumulated at the province level have positive impacts on renewable energy innovation of the non-CDM firms