Fog and Cloud Computing Assisted IoT Model Based Personal Emergency Monitoring and Diseases Prediction Services

Along with the rapid development of modern high-tech and the change of people's awareness of healthy life, the demand for personal healthcare services is gradually increasing. The rapid progress of information and communication technology and medical and bio technology not only improves personal healthcare services, but also brings the fact that the human being has entered the era of longevity. At present, there are many researches focused on various wearable sensing devices and implant devices and Internet of Things in order to capture personal daily life health information more conveniently and effectively, and significant results have been obtained, such as fog computing. To provide personal healthcare services, the fog and cloud computing is an effective solution for sharing health information. The health big data analysis model can provide personal health situation reports on a daily basis, and the gene sequencing can provide hereditary disease prediction. However, the injury mortality and emergency diseases since long ago caused death and great pain for the family. And there are no effective rescue methods to save precious lives and no methods to predict the disease morbidity likelihood. The purpose of this research is to capture personal daily health information based on sensors and monitoring emergency situations with the help of fog computing and mobile applications, and disease prediction based on cloud computing and big data analysis. Through the comparison of test results it was proved that the proposed emergency monitoring based on fog and cloud computing and the diseases prediction model based on big data analysis not only gain more of the rescue time than the traditional emergency treatment method, but they also accumulate lots of different personal healthcare related experience. The Taian 960 hospital of PLA and the Yanbian Hospital as IM testbed were joined to provide emergency monitoring tests, and to ensure the CVD and CVA morbidity likelihood medical big data analysis, the people around Taian city participated in personal health tests. Through the project, the five network layers architecture and integrated MAPE-K Model based EMDPS platform not only made the cooperation between hospitals feasible to deal with emergency situations, but also the Internet medicine for the disease prediction was built

Zhachong Shisanwei pill drug-containing serum protects H2O2-Induced PC12 cells injury by suppressing apoptosis, oxidative stress via regulating the MAPK signaling pathway

IntroductionZhachong Shisanwei Pill (ZSP) is a classical Mongolian formula that combines 13 types of Chinese medicinal materials and has been used for treating ischemic stroke (IS) for centuries. However, the underlying molecular mechanisms have yet to be fully elucidated. The aim of this study is to explore potential mechanism of ZSP on nerve cells in cerebral ischemic injury.MethodsTo simulate the pathological process of oxidative stress following IS, an injury model using PC12 cells was induced with hydrogen peroxide (H2O2). Afterward, PC12 cells were treated with ZSP medicated serum at low, medium, and high doses. Various assays were conducted to assess cell viability and oxidative stress indicators, including lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), reactive oxygen species (ROS), and mitochondrial membrane potential (MMP). Cell apoptosis was evaluated through morphological assessment and flow cytometry. Additionally, the expression levels of apoptosis-related proteins (Bcl-2, Bax, Caspase-9, Caspase-3, PARP) and signaling pathway proteins (JNK, phosphorylated JNK, ERK, phosphorylated ERK, p38, and phosphorylated p38) were measured using automated Western blotting.ResultsOur findings indicate that ZSP medicated serum preconditioning improves the condition of PC12 cells injured by H2O2. Specifically, it increased cell survival rates and reduced LDH release. Additionally, ZSP treatment decreased ROS levels and MDA content, while enhancing the activity of SOD and CAT in the injured PC12 cells. ZSP also reversed the depolarization of mitochondrial membrane potential and protected cells from apoptosis by modulating the expression of apoptosis-related proteins, including Bcl-2, Bax, Caspase-9, Caspase-3, and PARP. Furthermore, the overactivation of the MAPK signaling pathway due to H2O2-induced injury was inhibited, as evidenced by the downregulation of phosphorylated JNK, ERK, and p38 levels.DiscussionMongolian medicine ZSP demonstrates protective effects against H2O2-induced oxidative stress and apoptosis in PC12 cells. The underlying mechanism may involve the inhibition of the MAPK signaling pathway, enhancement of antioxidant enzyme activity, reduction of intracellular peroxidation levels, and suppression of intrinsic apoptosis pathways

Structural roles of amphiphilic peptide tails on silica biomineralization

By changing the tails of amphiphilic peptides, the nanostructures after silica biomineralization were varied due to the conformation changes.</p