Up-regulation of MiR-205 under hypoxia promotes epithelial-mesenchymal transition by targeting ASPP2

The epithelial–mesenchymal transition (EMT) is one of the crucial procedures for cancer invasion and distal metastasis. Despite undergoing intensive studies, the mechanisms underlying EMT remain to be completely elucidated. Here, we identified that apoptosis-stimulating protein of p53-2 (ASPP2) is a novel target of MiR-205 in various cancers. Interestingly, the binding site of MiR-205 at the 3′-untranslated region of ASPP2 was highly conserved among different species. An inverse correlation between MiR-205 and ASPP2 was further observed in vivo in cervical cancers, suggesting MiR-205 may be an important physiological inhibitor of ASPP2. Hypoxia is a hallmark of solid tumor microenvironment and one of such conditions to induce EMT. Notably, MiR-205 was remarkably induced by hypoxia in cervical and lung cancer cells. A marked suppression of ASPP2 was observed simultaneously. Further studies confirmed that hypoxia-induced ASPP2 suppression was mainly attributed to the elevated MiR-205. Interestingly, the alteration of MiR-205/ASPP2 under hypoxia was accompanied with the decreased epithelial marker E-cadherin and increased mesenchymal marker Vimentin, as well as a morphological transition from the typical cobblestone-like appearance to the mesenchymal-like structure. More importantly, MiR-205 mimics or ASPP2 silencing similarly promoted EMT process. By contrast, ASPP2 recovery or MiR-205 inhibitor reversed MiR-205-dependent EMT. Further studies demonstrated that the newly revealed MiR-205/ASPP2 axis promoted cell migration and also increased cell proliferation both in vivo and in vitro. These data together implicated a critical impact of MiR-205/ASPP2 on promoting EMT. MiR-205/ASPP2 may be potential diagnostic and therapeutic biomarkers in cervical and lung cancers

34. 上肢のentrapment neuropathy手術例について(第520回千葉医学会例会 整形外科例会)

<p>No.8 nozzle assembly; flow rate 15 L/s; ambient pressure 30 MPa; ambient temperature = jet temperature = 373 K. In cross sections, dimensionless radius is the distance from the inner wall to the measure point divided by the total length between inner and outer wall. The tangential velocity has large gradients near both sides of inner and outer walls.</p

A56: Mediation Analysis of Association Between Fundamental Motor Skills and Physical Activity in Elementary School Students

Purpose: This study aimed to investigate the association of fundamental motor skills (FMS), perceived motor competence (PMC), physical activity (PA) in elementary school students, as well as to examine whether PMC mediated the relationship between FMS and PA. Methods: Participants were 470 3-6th grade students (210 boys vs. 242 girls; Mean age = 10.11 years old; SD = 1.19) at five elementary schools from the province of Hunan in China. The students’ FMS were assessed with The Test of Gross Motor Development-3, PMC was assessed using the perceived athletic competence sub-scale of Harter’s Self-perception profile for children and PA were assessed using Physical Activity Questionnaire for Older Children, respectively. Data were analyzed with descriptive statistics, independent sample t test, Pearson correlation coefficients, multiple regressions, and bootstrapping method. Results: The descriptive statistics showed that the students’ PA was generally in the moderate level (M =2.78, SD = 0.75). The boys’ total FMS mean score (M =72.63, SD = 9.62), ball skills mean score (M = 39.33, SD = 6.23), PMC mean score (M =2.78, SD= 0.66), PA mean score (M = 2.97, SD = 0.75) was higher than the girls respectively. The independent sample t test showed that significant difference in the mean score of total FMS, ball skills, PMC, PA between the boys and girls (t = 3.99, p \u3c 0.001; t = 6.20, p \u3c 0.001; t = 5.40, p \u3c 0.001; t = 5.42, p \u3c 0.001) respectively. Also, the bivariate correlations coefficients showed that FMS, PMC, and PA were related to each other at p \u3c 0.01 level. The result of simple mediation analyses showed that PMC mediate the association between FMS and PA (Indirect effect = 0.07, 95%CI = [0.03, 0.11]). Conclusion: Good FMS levels are associated with a high level of PA. The relationship between FMS and PA was fully mediated by the PMC. This study suggests that improving FMS and maintaining appropriate levels of PMC may promote PA in elementary school students

Construction of MnO2 Nanowire for a High-Performance Lithium Ion Supercapacitor

Developing lithium ion capacitors possessing both brilliant energy and power density is still significant for numerous re-searchers. In this paper, we synthesized MnO2 nanowires via a simple hydrothermal process. The nanostructure MnO2 can expose more electrochemical sites and thus optimize the kinetics of Li+. Moreover, we used MnO2 nanowires (MnO2 NWs) as anode and a N-doped porous carbon (NPC) as cathode to assemble lithium ion capacitors (MnO2 NWs//NPC LIC). Compared to the traditional supercapacitor with aqueous electrolyte, the MnO2 NWs//NPC LIC exhibits a wider voltage of 0-4.2 V, which is helpful to enhance its energy and power density. Furthermore, MnO2 NWs//NPC LIC can deliver an excellent capacity of 150 mAh g-1 with an excellent energy density of 82.7 Wh kg-1 and power density of 1.05 kW kg-1. Meanwhile, a good cyclic stability of LICs with a 20% retention after 1000 times charge and discharge process proves its practical potential, indicating a good promising for the application in storage devices

Effect of Peripheral Administration of Kisspeptin-10 on Dynamic LH Secretion in Prepubertal Ewes

The aim of the present study was to clarify the effect of kisspeptin-10 on LH secretion in prepubertal ewes. In experiment 1, prepubertal ewes fitted with indwelling jugular catheters were randomly assigned to receive 0, 0.5, 1 or 2 mg of kisspeptin-10 dissolved in saline, and serial blood samples were collected at 15-min intervals for 180 min to analyze the response curves of LH after injection. In experiment 2, prepubertal ewes fitted with indwelling jugular catheters were injected with 0 or 1 mg of kisspeptin-10 dissolved in saline and the injection was repeated 3 times at 1 h interval and serial blood samples were collected at 15-min intervals for 210 min to analyze the response curves of LH after injection. The results showed that single intravenous administration of 0.5, 1 and 2 mg of kisspeptin-10 all could significantly increased LH secretion in prepubertal ewes, and the effect of 1 and 2 mg of kisspeptin-10 on LH secretion was higher than that of 0.5 mg group. The results also showed that repeated intravenous administration of kisspeptin-10 could effectively increase LH secretion and repeated administration did not influence the effect of kisspeptin-10 on LH secretion in prepubertal ewe. In conclusion, the present study indicated that single or repeated intravenous administration of kisspeptin-10 could effectively increase LH secretion in prepubertal ewes

The Average IFN- γ

Previous studies suggested that both the frequency and the mean fluorescence intensity (MFI) of cytokine secreting T cells could be of great value for immunogenicity evaluation of a vaccine. In this study, by constructing epitope-based DNA vaccines encoding a previously identified CD8+ T cell epitope, we investigated the influence of multiplying epitope copies on both the frequency and the MFI of specific IFN-γ secreting CD8+ T cells. We found that frequencies of specific CD8+ T cell could be improved by multiplying epitope copies, while the MFI of IFN-γ secreted by epitope-specific CD8+ T cells decreased synchronously. And further analysis showed that the decrease of MFI was not caused by the functional avidity variation of CD8+ T cell receptor

Physiological response of sunflowers to broomrape parasitism and analysis of the key metabolic pathways

[Objective] To study the physiological response of sunflowers to broomrape parasitism and the related metabolic pathways, as well as understanding the molecular mechanism of sunflower resistance to broomrape parasitism. [Methods] The broomrape-resisant (s41) and -sensitive (s26) sunflowers were used as the testing materials. Transcriptome analysis of 4 samples under the condition of parasitic stress was carried out, and physiological verification analysis of key metabolites was carried out. [Results] (1) Under inoculation, the plant height and root number of susceptible materials were affected significantly, and some of them were even unearthed, while the resistant materials had no obvious changes, and only a few were parasitized. (2) The number of DEGs was 6 362, 6 609, 5 490 and 5 469 by screening the differential genes among the samples. GO analysis showed that the four comparison groups, namely s26-s41, s26CK-s41CK, s26-s26CK, and s41-s41CK, had the most differential genes in biological processes, followed by cellular components, and less in molecular functions. KEGG analysis showed that infection had a great influence on plant signal transduction, biosynthesis of secondary metabolites, lipid metabolism, amino acid metabolism, and sugar metabolism in sunflowers. (3) Physiological analyses found that the phenylalanine deaminase, cinnamyl alcohol dehydrogenase, glutathione, total phenols, and salicylic acid showed an upward trend, indicating that the stress tolerant system was activated under parasitism. However, the trend of the physiological indicators of the sensitive and tolerant materials were different. Also, the genes controlling the physiological responses were differentially expressed. [Conclusion] It was found that inoculation with broomrape significantly affected the plant height and root volume of the susceptible materials, while the resistant materials were not affected. The resistant materials were enhanced in root lignin synthesis, antioxidant enzyme activity, and the expression of key genes of phenylpropanoid metabolism, salicylic acid and signaling pathway, and hence increased the resistance to broomrape

Genotypic spectrum of α-thalassemia and β-thalassemia in newborns of the Li minority in Hainan province, China

PurposeTo explore the genotypes and allele frequencies of α, β and α+β thalassemias in Li minorities, which resided in Hainan Province of China for a long time.MethodsIn the present study, 1,438 newborns of the Li minority were collected from January 2020 to April 2021. The genotypes of thalassemia were detected by fluorescence PCR and verified by flow-through hybridization PCR analyses. Rare genotypes were detected by restriction fragment length polymorphism electrophoresis and Sanger DNA sequencing.ResultsAmong 1,438 participants, 1,024 (71.2%) were diagnosed with any kind of thalassemia. Among all thalassemia carriers, 902 (88.09%) subjects were diagnosed with α-thalassemia, and 18 subtypes of α-thalassemia were detected, with the top three genotypes being −α4.2/αα (25.39%), −α3.7/αα (22.62%) and αWSα/αα (16.96%). Thirty-two (3.13%) patients were β-thalassemia carriers, and 6 types of β-thalassemia genotypes were detected. The top two genotypes were βCD41–42/βN (46.88%) and β−28/βN (18.75%). Additionally, 90 (8.79%) cases were α + β-thalassemia, and the top two genotypes were −α3.7/αα, βCD41–42/βN (30.00%) and −α4.2/αα, βCD41–42/βN (26.67%). Furthermore, two genotypes (−α4.2/HKαα and βCD76 GCT &gt; CCT/βN) were first identified in Hainan Province, and βCD76 GCT &gt; CCT/βN was first identified in China.ConclusionNewborns of Li have a higher prevalence of thalassemia for a long period, and further education on the impact of thalassemia, follow-up studies of the clinical manifestation and treatment and proper intervention methods should be designed to reduce the burden of thalassemia and enhance the quality of life in Li newborns