Use of autologous adipose-derived mesenchymal stem cells for creation of laryngeal cartilage

OBJECTIVES/HYPOTHESIS: Adipose-derived mesenchymal stem cells (ASCs) are an exciting potential cell source for tissue engineering because cells can be derived from the simple excision of autologous fat. This study introduces a novel approach for tissue-engineering cartilage from ASCs and a customized collagen oligomer solution, and demonstrates that the resultant cartilage can be used for laryngeal cartilage reconstruction in an animal model. STUDY DESIGN: Basic science experimental design. METHODS: ASCs were isolated from F344 rats, seeded in a customized collagen matrix, and cultured in chondrogenic differentiation medium for 1, 2, and 4 weeks until demonstrating cartilage-like characteristics in vitro. Large laryngeal cartilage defects were created in the F344 rat model, with the engineered cartilage used to replace the cartilage defects, and the rats followed for 1 to 3 months. Staining examined cellular morphology and cartilage-specific features. RESULTS: In vitro histological staining revealed rounded chondrocyte-appearing cells evenly residing throughout the customized collagen scaffold, with positive staining for cartilage-specific markers. The cartilage was used to successfully repair large cartilaginous defects in the rat model, with excellent functional results. CONCLUSIONS: This study is the first study to demonstrate, in an animal model, that ASCs cultured in a unique form of collagen oligomer can create functional cartilage-like grafts that can be successfully used for partial laryngeal cartilage replacement

Stem cell-derived tissue-engineered constructs for hemilaryngeal reconstruction

OBJECTIVES: As an initial step toward our goal of developing a completely tissue-engineered larynx, the aim of this study was to describe and compare three strategies of creating tissue-engineered muscle-polymer constructs for hemilaryngeal reconstruction. METHODS: Cartilage-mimicking polymer was developed from electrospun poly(D,L-lactide-co-ε-caprolactone) (PCL). Primary muscle progenitor cell cultures were derived from syngeneic F344 rat skeletal muscle biopsies. Twenty F344 rats underwent resection of the outer hemilaryngeal cartilage with the underlying laryngeal adductor muscle. The defects were repaired with muscle stem cell-derived muscle-PCL constructs (5 animals), myotube-derived muscle-PCL constructs (5 animals), motor end plate-expressing muscle-PCL constructs (5 animals), or PCL alone (controls; 5 animals). The outcome measures at 1 month included animal survival, muscle thickness, and innervation status as determined by electromyography and immunohistochemistry. RESULTS: All of the animals survived the 1-month implant period and had appropriate weight gain. The group that received motor end plate-expressing muscle-PCL constructs demonstrated the greatest muscle thickness and the strongest innervation, according to electromyographic activity and the percentage of motor end plates that had nerve contact. CONCLUSIONS: Although all of the tissue-engineered constructs provided effective reconstruction, those that expressed motor end plates before implantation yielded muscle that was more strongly innervated and viable. This finding suggests that this novel approach may be useful in the development of a tissue-engineered laryngeal replacement

Airway dimensions and pathologies of trumpet players vs. non-trumpet players.

Objective: The objective of this retrospective, 3-dimensional cone beam computed tomography study was to determine if there is a significant difference between the most constricted area of the airway, the prevertebral soft tissue thickness and airway dimensions (length and volume) of the nasal cavity, nasopharynx, oropharynx, and maxillary sinuses of university trumpet players versus non-trumpet playing controls. The second objective was to determine significant differences in the prevalence of airway pathologies between university trumpet players and controls. Method: Following IRB approval and consent and reliability studies, measurements of airway parameters and pathology were compared between 66 Caucasian trumpeters and 22 ethnicmatched controls. An analysis of covariance, with age and sex included as covariates, was used to compare the airway measures. Since there was a significant difference in gender and age, comparisons between groups for the presence of any airway pathologies was made using logistic regression including age and sex as covariates. A 5% significance level was used for all comparisons. Result: The trumpet players had significantly smaller nasal cavity volume (18028 + 595 mm3 vs. 25266 + 1116 mm3; p<0.0001) and significantly greater soft tissue thickness at CV2ia (3.29 + 0.10 mm vs 2.70 + 0.10 mm; p=0.03) and CV3sa (4.55 + 0.13 mm vs 3.74 + 0.14 mm; p=0.005) than the controls. No other airway measure demonstrated a significant difference between the two groups. Trumpeters had significantly (p=0.002) more airway pathology (n=33; 50%) than the controls (n=4; 18%). Antral pseudocysts or polyps composed 52% of trumpeter pathologies as compared with 0% controls. Conclusion: The only significant differences in airway dimensions between trumpet players and controls were decreased nasal cavity volume and some areas of prevertebral soft tissue thickness. Trumpeters were almost three times as likely to exhibit airway pathology when compared with controls. Funding: IUPUI Signature Center Initiative – 3D Imaging of the Craniofacial Complex Center and the Joseph and Aida Jarabak Endowed Professorship

Three-dimensional airway volumes and most constricted areas in children

poster abstractObjective: The objectives of this retrospective study using cone beam computed tomography (CBCT) were to determine if there are differences in the volume of various airway segments and the most constricted area (MCA) of children with different dentoskeletal patterns. Methods: The initial CBCTs of 83 orthodontic patients (30 Angle’s Class I; 26 Class II; and 27 Class III) were collected from a private orthodontic office. Following reliability studies, various parameters of the craniofacial complex, airway volume, and MCA were measured utilizing Dolphin 3D software. Comparisons among the three dental and the skeletal malocclusion classes were performed using one-way ANOVA and Fishers Protected Least Significant. Associations of the airway volumes and the MCA with other parameters were determined using correlation coefficients, accepting p < 0.05 as significant for all tests. Results: Maxillary right sinus volume was the only airway segment showing significant difference among different dental classes. Maxillary sinus volume also correlated moderately with anterior facial height and mandibular length. No significant differences were found between the MCA and different dentoskeletal classifications. Conclusions: The only significant difference in airway parameters among the dentoskeletal classes was that the dental Class II subjects had greater right sinus volume than the other classes. Shorter anterior facial height or mandibular length could be indicators for decreased airway volume in children. Funding: IUSD Graduate Research Committee, IUPUI Three-Dimensional Imaging of the Craniofacial Complex Center, Jarabak Endowed Professorshi

Peran Modal Psikologis dan Organizational Virtuousness terhadap Work Engagement Karyawan Generasi Milenial

Work engagement is one of job performance predictor, but millennial is known to have lower work engagement than older generation. Psychological capital and organizational virtuousness are some important factor to increase work engagement. The aim of this study was to determine psychological capital and organizational virtuousness as work engagement predictor. This research used quantitative approach. Questionnaires used to collect data about psychological capital, organizational virtuousness, and work engagement. Analyzed by multiple linier regression with 212 millennial worker who born between 1980 until 2000 as participant. The result of hypothesis test showed in the value of F=124,809 (p&lt;0,05), the hypothesis was accepted which explained that psychological capital and organizational virtuousness have a role as predictor of work engagement of millennial generation worker with contribution as much as 54,4%. These result provide support about improvement of work engagement through organizational virtuousness and psychological capital by optimizing leader role to giving coaching to the worker. Keyword: millennial, psychological capital, virtuousness, work engagemen

ANALYSIS OF THE LAJNAH BAHTSUL MASAIL NU FATWA METHOD ON DIVORCE JOKES

The Lajnah Bahtsul Masail issued a fatwa regarding jokes about divorce through NU Online. In this case, someone asked whether someone who jokes about divorce has incurred thalak, and NU Online provided an answer to this question. Lajnah Bahtsul Masa'il is an NU discussion forum that explores legal issues arising from the community. Bahtsul Masail. 'Bahtsul' refers to research, discussion, and exploration. Masail, on the other hand, refers to questions, issues, problems, cases and events. Thus, linguistically, 'bahtsul masail' can be interpreted as 'discussion of a problem'. This study aims to understand the Lajnah Bahtsul Masail method of issuing a law from a problem, particularly with regard to the issue of divorce jokes. The research method employed was a literature study involving data collection through the analysis of various scientific sources, such as journals and books. The results of the study show that Bahtsul Masail uses several methods to explore a law, including the Qauli, Ilhaqi, and Manhaji methods. When answering questions about the law of divorce jokes, Bahtsul Masail uses the Manhaji method, applying the rules of Imam As-Suyuti in the book Al-Asybah Wan Nazhair, although initially the answerer refers to the book Fathul MuinLajnah Bahtsul Masail mengeluarkan fatwa tentang hukum gurauan cerai melalui media NU Online. Dalam kasus ini, seseorang bertanya tentang bagaimana hukum tentang seseorang yang bergurau masalah cerai apakah sudah jatuh thalak, maka dalam hal ini NU Online merilis jawaban dari pertanyaan tersebut. Lajnah Bahtsul Masa’il merupakan forum didkusi Nahdatul Ulama untuk menggali hukum dari permasalahan-permasalahan yang datang dari masyarakat. Bahtsu dan Masail. Bahtsul memiliki arti penelitian, pembahasan, pencarian, riset, diskusi dan eksplorasi, sedangkan Masail mempunyai arti pertanyaan, persoalan, isu, problematika, perkara dan kejadian. Maka bahtsul masail secara bahasa yakni pembahasan sebuah persoalan. Penelitian ini bertujuan untuk memahami bagaimana metode Lajnah Bahtsul Masail dalam mengeluarkan suatu hukum dari suatu masalah terutama masalah yang dibahas yaitu tentang hukum gurauan cerai. Metode yang digunakan adalah studi kepustakaan, yaitu pengumpulan data melalui analisis dari berbagai sumber ilmiah seperti jurnal dan buku. Hasil dari penelitian menunjukkan bahwa bahtsul masail menggunakan beberapa metode dalam menggali suatu hukun, diantaranya adalah metode Qauli, metode Ilhaqi dan Metode Manhaji. Dalam menjawab pertanyaan tentang hukum gurauan cerai, Bahtsul Masail menggunakan metode Manhaji dengan menggunakan kaidah dari Imam As-Suyuti dalam kitab Al-Asybah Wan Nazhair sekalipun diawal penjawab merujuk pada kitab Fathul Muin

Rancang Bangun Sistem Pengenal Karakter Brille Dengan Metode Local Binary Pattern

Braille adalah media yang digunakan tunanetra untuk kebutuhan media baca tulis. Pola karakter untuk merepresentasikan huruf, angka dan tanda baca lainnya dilambangkan dengan pola titik-titik yang tersusun pada 6 titik posisi untuk setiap karakter yang dinamakan karakter braille. Pada tugas akhir ini dirancang sistem yang dapat mengenali dan menerjemahkan susunan karakter braille pada media kertas dengan metode local binary pattern, metode yang digunakan akan diujikan untuk mengetahui seberapa sesuai metode tersebut untuk digunakan pada pengenalan karakter braille. Sistem yang dirancang terdiri dari dua bagian yaitu sistem perangkat keras dan perangkat lunak. Hasil yang didapat melalui metode local binary pattern kemudian akan dibandingkan dengan metode lain (evaluasi piksel terprediksi) untuk membandingkan performa setiap metode. Pada sistem perangkat keras digunakan raspberry pi 3 model B, sedangkan pada sistem perangkat lunak digunakan library opencv yang memiliki fungsi-fungsi pengolahan data citra digital. Hasil dari tugas akhir ini menunjukkan bahwa dengan menggunakan metode LBP untuk mendapatkan hasil yang baik maka dibutuhkan entri data learning yang cukup banyak dan berefek pada tempo kalkulasi yang dibutuhkan menjadi lebih lama, sedangkan hasil dari metode yang tidak menyertakan LBP dan data learning menunjukkan hasil yang lebih baik dan tempo kalkulasi yang lebih cepat. Dengan metode LBP 3 data learning didapatkan persentase pengenalan karakter benar sebesar 79,855 %, dengan metode LBP 6 data learning 82,294 %, sedangkan dengan metode lain sebesar 94,399 %. Hal ini menunjukkan bahwa performa metode lain yang digunakan sebagai pembanding relatif lebih baik dibanding metode LBP. ========================================================================================= Braille is media used by the blind in order to read and write. Characters which are used are different to the common media that is used by normal people, the way it represents a character such as letters, numbers and punctuations is through some bulges occupying on six possible positions, it is called braille character. In this final project a system has been designed and built to has ability to recognizes and translates braille character on a paper-printed media into an ASCII code using local binary pattern method, the method will be tested and compared by another mehod (predicted pixel evaluation) to figures out how convenient the method is to recognizes the character. The system includes two part of subsytems, the firts one is hardware system. It is built to captures some images of the paper-printed media and the second one is software system or the codes, it is coded to processes the images datas, recognizes characters in it and translates them into ASCII codes. After the result has been gotten, it will be compared with another method to see both performaces. The hardware system is equipped with mini computer raspberry pi 3 model B, while the software system uses openCV computer vision library which contains many functions that perform digital image data processing. The result of this final project shows that by using LBP method to get good result, it needs a lot of learning data entries and effects to calculation time needed to be longer, whereas the result from the other method shows better result and faster in calculation time. The quantitative result of right recognition obtained from LBP method with 3 datas learning is 79,855 %, LBP with 6 datas learning is 82,294 %, and another method is 94,399 %. This shows that the another method has better performance than LBP itself

PENGARUH ULASAN ONLINE TRIPADVISOR TERHADAP KEPUTUSAN MENGINAP (Survei terhadap generasi milenial di Kota Bandung)

Penelitian ini dilakukan dengan tujuan untuk menguji pengaruh ulasan online Tripadvisor terhadap keputusan menginap. Ulasan online sebagai variabel bebas pada penelitian ini memiliki beberapa sub dimensi, yaitu general persuasiveness, perception of review credibility, perceived quality, cognitive attribute, affective attribute, dan sensory attribute sedangkan keputusan menginap merupakan variable terikat. Teknik pengambilan sampel yang digunakan yaitu purposive sampling dan kuisioner diajukan kepada 120 responden menggunakan media google form. Teknik analisis data yang digunakan adalah regresi linear berganda dengan bantuan perangkat IBM SPSS Statistic 24 dan Microsoft Excel. Hasil analisis menunjukan secara simultan variabel ulasan online memiliki pengaruh terhadap keputusan menginap sebessar 56.7%, akan tetapi secara parsial hanya terdapat 3 sub dimensi ulasan online yang memiliki pengaruh terhadap keputusan menginap yaitu perceived quality, affective attribute, dan sensory attribute. Hal ini membuktikan ulasan online memberikan pengaruh terhadap keputusan menginap. This study attempts to determine the influence of online Tripadvisor reviews on customers’ decision-making. Online reviews as the independent variable in this study consist of some sub-dimensions, i.e., general persuasiveness, perception of review credibility, perceived quality, cognitive attribute, affective attribute, and sensory attribute. A purposive sampling technique was used in this study and a set of questionnaires was administered to 120 respondents using the Google form. This study used the multiple linear regression technique by utilizing IBM SPSS Statistic 24 and Microsoft Excel. This study reveals that the online reviews variable simultaneously influenced the customers’ booking intention as much as 56.7%, but partially there were only 3 sub-dimensions of online reviews that had an influence on the customers’ intention such as perceived quality, affective attribute, and sensory attribute. These results proved that online reviews influenced customers’ booking intention