The effect of 3-indoleacetic acid on the response of Lactobacillus arabinosus 17-5 to nicotinamide

Lactobacillus arabinosus 17-5 has been widely used as an assay organism for nicotinic acid (NA) since the development of the method by Snell and Wright (1). Although it has been realized that other substances present in tissue extracts may interfere with the bioassay, the nature of such substances has not been elucidated. During an investigation of tryptophan metabolism in the pea plant, we studied the possible conversion of this compound to nicotinic acid, since such a transformation has been demonstrated to occur in numerous organisms (2-4). The method involved infiltration of tissue with large quantities of tryptophan and subsequent bioassay for nicotinic acid with Lactobacillus arabinosus 17-5. Certain anomalous results led us to believe that other metabolites of tryptophan were interfering with the assay. Because 3-indoleacetic acid (IAA) is a known plant metabolite of tryptophan (5), we tested it for possible interference with the assay, and, as described below, found that such interference may occur under certain circumstances

Spatially Resolved Spectroscopy of Europa: The Distinct Spectrum of Large-scale Chaos

We present a comprehensive analysis of spatially resolved moderate spectral resolution near-infrared spectra obtained with the adaptive optics system at the Keck Observatory. We identify three compositionally distinct end member regions: the trailing hemisphere bullseye, the leading hemisphere upper latitudes, and a third component associated with leading hemisphere chaos units. We interpret the composition of the three end member regions to be dominated by irradiation products, water ice, and evaporite deposits or salt brines, respectively. The third component is associated with geological features and distinct from the geography of irradiation, suggesting an endogenous identity. Identifying the endogenous composition is of particular interest for revealing the subsurface composition. However, its spectrum is not consistent with linear mixtures of the salt minerals previously considered relevant to Europa. The spectrum of this component is distinguished by distorted hydration features rather than distinct spectral features, indicating hydrated minerals but making unique identification difficult. In particular, it lacks features common to hydrated sulfate minerals, challenging the traditional view of an endogenous salty component dominated by Mg-sulfates. Chloride evaporite deposits are one possible alternative

Polarisation observables in lepton antilepton to proton antiproton reactions including lepton mass

General expressions, including the lepton mass, for the spin averaged differential cross section for the annihilation reaction lepton antilepton to proton antiproton are given, as well as general formulae for the single and double spin asymmetries in the centre of mass frame. In particular we discuss the single spin asymmetry, normal to the scattering plane, which measures the relative phase difference between nucleon electromagnetic form factors $G_E$ and $G_M$. Recent experimental investigations of these form factors in the space and time like region are reviewed. It is thought that measurements of the phase of these form factors will provide fundamental information on the internal nucleon structure. The phases between $G_E$ and $G_M$ are accessible through polarisation observables measured in the antiproton proton to lepton antilepton reaction, or in its time reversed process.Comment: 14 pages, to be submitted to EPJ

Resource Provisioning for Multi-Tier Virtualized Server Applications

Virtualizing the x86-based data center creates a dynamic environment for server application deployment and resource sharing. Resource management in this environment is challenging as applications are under fluctuating workloads causing diverse resource demands across their tiers. Resource allocation adaptation is essential for high performance machine utilization. This paper presents feedback controllers that dynamically adjust the CPU allocations of multi-tier applications in order to adapt to workload changes by considering the resource coupling between utilizations of application components. Our experimental evaluation on a virtualized 3-tier Rubis server application shows that our techniques work effectively