Transcriptome profile analysis of cell proliferation molecular processes during multicellular trichome formation induced by tomato Wo v gene in tobacco

Trichomes, developing from the epidermis of nearly all terrestrial plants, provide good protection from environmental stress. Regulation of trichomes in Rosids has been well characterized. However, little is known about the cell proliferation molecular processes during multicellular trichome formation in Asterids. Ectopic expression of Wov in tobacco and potato induces much more trichome formation than wild type. To gain new insights into the underlying mechanisms during the processes of these trichomes formation, RNA-seq was employed for the young primary leaf tissues in Wov transgenic and wild-type tobacco. We identified differentially expressed genes which are related to various biological processes and molecular functions. Here, we provide details of experimental methods, RNA-seq data (available at Gene Expression Omnibus database under GSE72310). Our data provide new insight into the molecular processes controlling multicellular formation in tobacco

Transcriptional profiles of drought-responsive genes in modulating transcription signal transduction, and biochemical pathways in tomato

To unravel the molecular mechanisms of drought responses in tomato, gene expression profiles of two drought-tolerant lines identified from a population of Solanum pennellii introgression lines, and the recurrent parent S. lycopersicum cv. M82, a drought-sensitive cultivar, were investigated under drought stress using tomato microarrays. Around 400 genes identified were responsive to drought stress only in the drought-tolerant lines. These changes in genes expression are most likely caused by the two inserted chromosome segments of S. pennellii, which possibly contain drought-tolerance quantitative trait loci (QTLs). Among these genes are a number of transcription factors and signalling proteins which could be global regulators involved in the tomato responses to drought stress. Genes involved in organism growth and development processes were also specifically regulated by drought stress, including those controlling cell wall structure, wax biosynthesis, and plant height. Moreover, key enzymes in the pathways of gluconeogenesis (fructose-bisphosphate aldolase), purine and pyrimidine nucleotide biosynthesis (adenylate kinase), tryptophan degradation (aldehyde oxidase), starch degradation (β-amylase), methionine biosynthesis (cystathionine β-lyase), and the removal of superoxide radicals (catalase) were also specifically affected by drought stress. These results indicated that tomato plants could adapt to water-deficit conditions through decreasing energy dissipation, increasing ATP energy provision, and reducing oxidative damage. The drought-responsive genes identified in this study could provide further information for understanding the mechanisms of drought tolerance in tomato

Can Digital Finance Enable China’s Industrial Carbon Unlocking under Environmental Regulatory Constraints? Joint Tests of Regression Analysis and Qualitative Comparative Analysis

Sustainable development goals challenge the carbon lock-in dilemma of the industrial economy, and identifying the motivation and mechanism behind carbon unlocking has become an urgent priority. With its inclusive and precise advantages, digital finance (DF) provides a new impetus for the economy’s low-carbon transformation, while reasonable environmental regulation (ER) acts as an important guiding constraint. We focus on the carbon unlocking performance of DF under ER constraints. After constructing and calculating the industrial carbon unlocking efficiency (ICUE), we observe the trends of ICUE fluctuating positively, clustering towards the eastern region, and polarization. Subsequently, based on theoretical analyses, we explore the marginal and configuration effects of DF and ER in improving ICUE using panel data from 30 Chinese provinces between 2011 and 2021 and adopt a mixed research method with regression analysis (Tobit hierarchical regression and quantile regression for panel data (QRPD)) and dynamic fuzzy-set qualitative comparative analysis (fsQCA). The regression analysis results show that DF can notably enhance China’s provincial ICUE, with ER generally serving as a positive moderator; however, the unlocking potential of informal environmental regulations needs further exploration. As ICUE improves in a specific location or time, the positive contribution of DF to ICUE also increases, whereas the moderating effect of ER exhibits an optimal range and follows an inverted U-shape. The dynamic fsQCA results support the findings of the regression analysis and further emphasize that effective cooperation between DF and ER is crucial for high ICUE, while inadequate DF support and the absence of formal environmental regulations remain bottlenecks in industrial carbon lock-in. Moreover, configuration paths demonstrate clear path dependency in both time and space, indicating a prolonged unlocking endeavor

A perennial living mulch system fosters a more diverse and balanced soil bacterial community.

Cover crops are known to positively impact soil health, both at a physical level (through erosion control and organic matter enhancement) and at a biological level (by fostering more diverse microbial communities). However, most research in this area has been conducted in the context of annual cover crops that are terminated when the main crop is planted. We have previously demonstrated that a continuous "living mulch" cover crop system can enhance the physical and chemical aspects of soil health; In this study, we reveal its effect on the soil bacterial community and compare it to two different annual cover crops and a conventional control without cover crops. We examined the effect of a living-mulch (LM) system using perennial white clover (Trifolium pratense L), annual cereal rye (Secale cereale L.) (CR), annual crimson clover (Trifolium incarnatum L.) (CC), and a no-cover (NC) control at three time points during the 2018 growing season. 16S rRNA amplicon analysis of the soil bacterial community revealed that the community composition in cover crop systems was significantly different from the NC control, and that LM and CR accommodated more heterogeneous and even bacterial communities compared to the NC control. The difference in bacterial composition between cover crop systems appears to be partly influenced by soil nitrogen concentration and lime buffer capacity. Overall community diversity was associated with nitrogen and metal ion concentrations, and these associations were both stronger and more numerous later in the season. These results elucidate how a perennial cover crop system affects the soil bacterial community and advance our understanding of the interactions between crops, management practices, and soil microbiomes in sustainable agriculture

Fig 3 -

The linear regression between the distances in soil Nitrogen concentration (top) or Lime Buffer Capacity equilibrium value (bottom) against weighted UniFrac distance in June (left) and August (right) samples.</p