BPE-P04: the effect of plant oils for submerged fermentation of Schizophyllum commune producing mycelium biomass and exopolysaccharides

The effect of plant oils (olive, palm, corn, sunflower and soya bean) addition at different concentrations (0.5%. 1.0%, 2.0% and 4.0%) producing mycelium biomass (MBS) and exopolysaccharides (EPS) in submerged fermentation using a local strain Schizophyllum commune was determined. Additional of all type of plant oils exhibit higher yield of MBS and EPS than without plant oils. The highest production of MBS was obtained at 26.08 g/L in 4.0% palm oil, while in control (without plant oils) gave only 12.16 g/L of MBS. The maximum concentration of EPS was produced is 2.2 g/L from medium containing 1.0% and 4.0% of olive oil; and 2.0% of sunflower oil. The best treatment containing 4.0% of corn oil was selected since it showed high production of MBS (23.44 g/L) and EPS (2.1 g/L) compared to other treatments. However, the addition of 4.0% of palm oil in submerged culture of local strain Schizophyllum commune was preferred since it also showed the good result on production of MBS (26.08 g/L) and EPS (2.0 g/L). Palm oil is locally source and cheap, therefore, the MBS can be produced in local industry with cost-effective. The MBS will be used as liquid spawn (seed) or extraction of bioactive compounds in pharmaceutical industry

BPE-P01: Optimization of media for mass production of Schizophyllum commune using response surface methodology (RSM)

Schizophyllum commune (SC) is an edible mushroom which can be found worldwide except Antactica area. This type of mushroom has attracted attention because of its medicinal important. Optimization of the media composition is required for the higher production of SC mycelium mass in order to meet the demand particularly in pharmaceutical industries. Glucose and yeast extract was selected as carbon and nitrogen sources for the growth of this fungus and were optimised firstly using OFAT (one factor at a time) method. This media composition were then optimized using a response surface methods (RSM) to find the best composition of factors such as inoculum (seed), carbon, nitrogen concentration to produce a maximum mycelium mass as a response. Thirty set of different range medium composition were conducted by RSM design using Design Expert software. The statistical analysis showed that optimum media containing 11.7 % (v/v) of inoculum, 27g glucose and 1.2g yeast extract has given maximum production of mycelium mass where the yields of mycelium mass increased from 6.148g/l (unoptimized) to 15.68g/l in medium (optimized

An ethnobotanical study of traditional steam-bathing by the Batak people of North Sumatra, Indonesia

This study aimed to document (1) the Batak people’s knowledge of the use of medicinal plants for steambathing, (2) the preparation and operation of steam-bathing, and (3) the benefits of steam-bathing. To attain these objectives, data were collected by using ethnobotanical survey and interview methods. The survey was conducted in Kabanjahe and Berastagi traditional markets, in Kaban Tua village, and in Tanjung Julu village. The participants for the interview were nine medicinal plants traders, nine midwives, and 32 mothers. The basic principle of steam-bathing by the Batak people is based on thermotherapy and aromatherapy. A total of 59 species (belonging to 37 genera and to 25 families) have been documented as medicinal plants for their use as steam-bathing materials by the Batak people. The traders, midwives and mothers are all aware of the benefits of steam-bathing. Gaultheria leucocarpa Blume and Cinnamomum porrectum (Roxb.), the species that produce distinctive aromas and reduce pain, would be interesting to study for their phytochemical and pharmacological properties