511 research outputs found

    Understanding methods of wound debridement

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    Autolytic debridement describes the body's natural method of wound-bed cleansing, helping it to prepare the wound bed for healing. In acute wounds, autolytic debridement occurs automatically and often does not require intervention, as during the inflammatory stage of a wound, neutrophils and macrophages digest and removes devitalised tissue, cell debris and contaminants, clearing the wound of any cellular barriers to healing. In chronic wounds, by contrast, healing is often delayed, frequently because of inadequate debridement. The autolytic process becomes overwhelmed by high levels of endotoxins released from damaged tissue (Broadus, 2013). Therefore wound debridement becomes an integral part of chronic-wound management and practitioners involved in wound care must be fully competent at wound-bed assessment and have an awareness of the options available for debridement. This article will review wound-bed assessment, highlighting variations in devitalised tissue, and explore options available for wound debridement, taking into consideration patients’ pain and quality of life

    Differential expression of cyclin-dependent kinases in the adult human retina in relation to CDK inhibitor retinotoxicity

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    Cyclin-dependent kinases (CDKs) are a family of kinases associated predominantly with cell cycle control, making CDK inhibitors interesting candidates for anti-cancer therapeutics. However, retinal toxicity (loss of photoreceptors) has been associated with CDK inhibitors, including the pan-CDK inhibitor AG-012896. The purpose of this research was to use a novel planar sectioning technique to determine CDK expression profiles in the ex vivo human retina with the aim of identifying isoforms responsible for CDK retinotoxicity. Four CDK isoforms (CDK11, 16, 17 and 18) were selected as a result of IC50 data comparing neurotoxic (AG-012986 and NVP-1) and non-neurotoxic (dinaciclib and NVP-2) CDK inhibitors, with IC50s at CDK11 showing a clear difference between the neurotoxic and non-neurotoxic drugs. CDK11 was maximally expressed in the photoreceptor layer, whereas CDK16, 17 and 18 showed maximal expression in the inner nuclear layer. CDK5 (an isoform associated with retinal homeostasis) was maximally expressed in the retinal ganglion cell layer. Apart from CDK18, each isoform showed expression in the photoreceptor layer. The human Müller cell line MIO-M1 expressed CDK5, 11, 16 and 17 and AG-01298 (0.02–60 µM) caused a dose-dependent increase in MIO-M1 cell death. In conclusion, CDK11 appears the most likely candidate for mediation of photoreceptor toxicity. RNA profiling can be used to determine the distribution of genes of interest in relation to retinal toxicity in the human retina

    Condición Física para la salud en estudiantes de IV año turno regular de la Licenciatura en Fisioterapia, Instituto Politécnico de la Salud, UNAN-Managua, II semestre 2015

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    La condición Física para la Salud está relacionada con un buen estado de salud dirigido a prevenir factores de riesgo, estos pueden ser modificados mediante la actividad física o el ejercicio. El presente estudio tuvo como objetivo valorar condición física para la salud en estudiantes de IV año turno regular de la Licenciatura en Fisioterapia, Instituto Politécnico de la Salud, UNAN-Managua, II semestre 2015, para esto se realizó con una pre y post evaluación a través de la implementación de un programa de Actividad Física para mejorar la condición de salud de los participantes. Obtuvo un enfoque de tipo mixto, según los resultados es descriptivo-explicativo, de tipo prospectivo y de corte transversal. Para recolectar información se tomó en cuenta ficha de datos demográficos donde prevaleció con un 80% el sexo femenino entre las edades de 19 a 24 años, a través de una encuesta auto-administrada a los participantes se determinó el nivel, la frecuencia, el tiempo y tipo de actividad física que realizan habitualmente los participantes. Así mismo se procedió a la valoración de la composición corporal, la cual incluyó cálculo de índice de masa corporal y porcentaje de grasa corporal, mediante mediciones antropométricas, también se evaluó resistencia cardiovascular a través de Escala Borg, tomando en cuenta constantes vitales, como presión arterial, frecuencia cardíaca y frecuencia respiratoria. Conociendo estos aspectos se desarrollaron actividades libres, ejercicios cardiovasculares, aeróbicos, juegos de competencia y de resistencia, los días martes, jueves y viernes, contemplados dentro del programa de actividad física, de los cuales se obtuvieron resultados positivos comprobados en la post evaluación del estudio. Palabras Clave: Condición física, Salud, Obesidad, Índice de masa Corporal, Porcentaje de Grasa, Resistencia Cardiovascular

    Exploring the occupational experiences of livestock farmers during drought: A narrative inquiry

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    Introduction: For much of the 21st century, Australia has endured the most prolonged and severe drought since records began. This drought has been found to have negative and long-lasting consequences upon both the physical and mental well-being of farmers and their families. To date, however, no research has examined the experience of drought from an occupational perspective. Objective: This study aims to explore the way in which drought impacts upon the lived experience of the farming role and the way in which the occupational identity of farmers influences the meanings attributed, and response to, drought. Methods: Narrative inquiry and thematic analysis were used to explore the experiences of drought among six farming men and four farming women from Northern Queensland. Findings: Four inter-related themes were identified. These being: ‘Becoming a farmer – Rites of passage on entry to the farming role’; ‘Farmers as guardians over the land’; ‘Drought as siege’ and; ‘Leisure occupations as temporary bridges to a world beyond drought’. Each of these themes offer insights into the way in which drought comes to be understood by farmers and, in turn, experienced and responded to. Conclusion: By better understanding the occupational experiences of farmers during drought, resources can be more effectively targeted towards promoting occupational balance and well-being. Interventions aimed at reframing the way in which the farming role is conceptualised from a young age and supporting occupations beyond farming as bridges to the outside world may be effective in achieving positive outcomes during drought

    Influence of seabird nutrient input on intertidal ecology in the Farne Islands, Northumberland

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    Seabirds are keystone species, providing inter-habitat connectivity by transporting vast quantities of nutrients from their pelagic feeding grounds to terrestrial and marine ecosystems, dictating community structure and dynamics, and providing multiple ecosystem services. This flow of nutrients is being disrupted by human activities, such as invasive species, and seabirds are now one of the most threatened bird groups. Whether seabird nutrients are cycling through nearshore, temperate systems, in particular in the United Kingdom (UK), impacting biodiversity and productivity, and how these inputs may vary temporally, remains unknown. The impact of nutrient subsidies to the Farne Islands, Northumberland, areas of high seabird densities during breeding season, was compared to nearby low seabird density areas on the Northumberland coast. Seabird densities and nitrogen inputs of up to 2334 and 1054 times higher, respectively, on seabird islands resulted in both substantially enriched nitrogen stable isotope (δ15N) values and higher total nitrogen content in terrestrial and intertidal organisms, including soil, terrestrial plants, lichen, barnacles, and limpets, and just higher δ15N values in macroalgae and turf algae. Temporal variation was also detected, though, surprisingly, nitrogen loads were higher during early breeding season. Some support was found for increased biodiversity in intertidal areas of seabird islands, in higher densities of intertidal predators and marginally greater algal species richness, though diversity differences were small overall. A marginal difference in limpet size was found, whilst on plots artificially cleared to simulate a disturbance event, barnacles were unexpectedly found to recolonise faster on mainland sites, which was likely caused by the mainland’s increased exposure level. These findings provide evidence that seabirds are cycling pelagic nutrients into terrestrial and intertidal ecosystems on UK islands, though support for seabird nutrient input increasing biodiversity and productivity was mixed. Given the threats to seabirds globally, these findings promote seabird conservation, including the removal of invasive species, such as rat eradication programmes
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