Determination of association constants between 5 '-guanosine monophosphate gel and aromatic compounds by capillary electrophoresis

Hydro gel formed by 5'-guanosine monophosphate (GMP) in the presence of a potassium ion is expected to exhibit interesting selectivity in capillary electrophoretic separations. Here, we estimated the conditional association constants between the hydro gel (G-gel) and aromatic compounds by capillary electrophoresis in order to investigate the separation selectivity that is induced by the G-gel. Several aromatic compounds were separated in a solution containing GMP and potassium ion at different concentrations. The association constants were calculated by correlating the electrophoretic mobilities of the analytes obtained experimentally using a concentration of G-gel. During semi-quantitative estimation, naphthalene derivatives had larger association constants (K-ass = 10.3-16.8) compared with those of benzene derivatives (K-ass = 3.91-5.31), which means that the binding sites of G-gel match better to a naphthalene ring than to a benzene ring. A hydrophobic interaction was also found when the association constants for alkyl resorcinol were compared with those of different hydrocarbon chains. The association constants of nucleobases and tryptophan ranged from 6.05 to 12.6, which approximated the intermediate values between benzene and naphthalene derivatives. Consequently, the selective interaction between G-gel and aromatic compounds was classified as one of three types: (1) an intercalation into stacked planar GMP tetramers; (2) a hydrophobic interaction with a long alkyl chain; or, (3) a small contribution of steric hindrance and/or hydrogen bonding with functional groups such as amino and hydroxyl groups

Urban community gardeners' knowledge and perceptions of soil contaminant risks

Although urban community gardening can offer health, social, environmental, and economic benefits, these benefits must be weighed against the potential health risks stemming from exposure to contaminants such as heavy metals and organic chemicals that may be present in urban soils. Individuals who garden at or eat food grown in contaminated urban garden sites may be at risk of exposure to such contaminants. Gardeners may be unaware of these risks and how to manage them. We used a mixed quantitative/qualitative research approach to characterize urban community gardeners' knowledge and perceptions of risks related to soil contaminant exposure. We conducted surveys with 70 gardeners from 15 community gardens in Baltimore, Maryland, and semi-structured interviews with 18 key informants knowledgeable about community gardening and soil contamination in Baltimore. We identified a range of factors, challenges, and needs related to Baltimore community gardeners' perceptions of risk related to soil contamination, including low levels of concern and inconsistent levels of knowledge about heavy metal and organic chemical contaminants, barriers to investigating a garden site's history and conducting soil tests, limited knowledge of best practices for reducing exposure, and a need for clear and concise information on how best to prevent and manage soil contamination. Key informants discussed various strategies for developing and disseminating educational materials to gardeners. For some challenges, such as barriers to conducting site history and soil tests, some informants recommended city-wide interventions that bypass the need for gardener knowledge altogether

The RR Lyrae Delay-Time Distribution: A Novel Perspective on Models of Old Stellar Populations

The delay-time distribution (DTD) is the occurrence rate of a class of objects as a function of time after a hypothetical burst of star formation. DTDs are mainly used as a statistical test of stellar evolution scenarios for supernova progenitors, but they can be applied to many other classes of astronomical objects. We calculate the first DTD for RR Lyrae variables using 29,810 RR Lyrae from the OGLE-IV survey and a map of the stellar-age distribution (SAD) in the Large Magellanic Cloud (LMC). We find that $\sim 46\%$ of the OGLE-IV RR Lyrae are associated with delay-times older than 8 Gyr (main-sequence progenitor masses less than 1 M$_{\odot}$), and consistent with existing constraints on their ages, but surprisingly about $51\%$ of RR Lyrae appear have delay times $1.2-8$ Gyr (main-sequence masses between $1 - 2$ M$_{\odot}$ at LMC metallicity). This intermediate-age signal also persists outside the Bar-region where crowding is less of a concern, and we verified that without this signal, the spatial distribution of the OGLE-IV RR Lyrae is inconsistent with the SAD map of the LMC. Since an intermediate-age RR Lyrae channel is in tension with the lack of RR Lyrae in intermediate-age clusters (noting issues with small-number statistics), and the age-metallicity constraints of LMC stars, our DTD result possibly indicates that systematic uncertainties may still exist in SAD measurements of old-stellar populations, perhaps stemming from the construction methodology or the stellar evolution models used. We described tests to further investigate this issue.Comment: 21 pages, 11 figures. Accepted to Ap

Manganese in residential drinking water from a community-initiated case study in Massachusetts

Background: Manganese (Mn) is a metal commonly found in drinking water, but the level that is safe for consumption is unknown. In the United States (U.S.), Mn is not regulated in drinking water and data on water Mn concentrations are temporally and spatially sparse. Objective: Examine temporal and spatial variability of Mn concentrations in repeated tap water samples in a case study of Holliston, Massachusetts (MA), U.S., where drinking water is pumped from shallow aquifers that are vulnerable to Mn contamination. Methods: We collected 79 residential tap water samples from 21 households between September 2018 and December 2019. Mn concentrations were measured using inductively coupled plasma mass spectrometry. We calculated descriptive statistics and percent of samples exceeding aesthetic (secondary maximum containment level; SMCL) and lifetime health advisory (LHA) guidelines of 50 µg/L and 300 µg/L, respectively. We compared these concentrations to concurrent and historic water Mn concentrations from publicly available data across MA. Results: The median Mn concentration in Holliston residential tap water was 2.3 µg/L and levels were highly variable (range: 0.03–5,301.8 µg/L). Mn concentrations exceeded the SMCL and LHA in 14% and 12% of samples, respectively. Based on publicly available data across MA from 1994–2022, median Mn concentration was 17.0 µg/L (N = 37,210; range: 1–159,000 µg/L). On average 40% of samples each year exceeded the SMCL and 9% exceeded the LHA. Samples from publicly available data were not evenly distributed between MA towns or across sampling years. Impact statement: This study is one of the first to examine Mn concentrations in drinking water both spatially and temporally in the U.S. Findings suggest that concentrations of Mn in drinking water frequently exceed current guidelines and occur at concentrations shown to be associated with adverse health outcomes, especially for vulnerable and susceptible subpopulations like children. Future studies that comprehensively examine exposure to Mn in drinking water and its associations with children’s health are needed to protect public health. © 2023, The Author(s)

Estimating State-Specific Contributions to PM2.5- and O3-Related Health Burden from Residential Combustion and Electricity Generating Unit Emissions in the United States

BACKGROUND: Residential combustion (RC) and electricity generating unit (EGU) emissions adversely impact air quality and human health by increasing ambient concentrations of fine particulate matter (PM2.5) and ozone (O3). Studies to date have not isolated contributing emissions by state of origin (source-state), which is necessary for policy makers to determine efficient strategies to decrease health impacts. OBJECTIVES: In this study, we aimed to estimate health impacts (premature mortalities) attributable to PM2.5 and O3 from RC and EGU emissions by precursor species, source sector, and source-state in the continental United States for 2005. METHODS: We used the Community Multiscale Air Quality model employing the decoupled direct method to quantify changes in air quality and epidemiological evidence to determine concentration-response functions to calculate associated health impacts. RESULTS: We estimated 21,000 premature mortalities per year from EGU emissions, driven by sulfur dioxide emissions forming PM2.5. More than half of EGU health impacts are attributable to emissions from eight states with significant coal combustion and large downwind populations. We estimate 10,000 premature mortalities per year from RC emissions, driven by primary PM2.5 emissions. States with large populations and significant residential wood combustion dominate RC health impacts. Annual mortality risk per thousand tons of precursor emissions (health damage functions) varied significantly across source-states for both source sectors and all precursor pollutants. CONCLUSIONS: Our findings reinforce the importance of pollutant-specific, location-specific, and source-specific models of health impacts in design of health-risk minimizing emissions control policies. Citation: Penn SL, Arunachalam S, Woody M, Heiger-Bernays W, Tripodis Y, Levy JI. 2017. Estimating state-specific contributions to PM2.5- and O3-related health burden from residential combustion and electricity generating unit emissions in the United States. Environ Health Perspect 125:324-332; http://dx.doi.org/10.1289/EHP550

Reading Between the (Spectral) Lines: Magellan/IMACS spectroscopy of the Ultra-faint Dwarf Galaxies Eridanus IV and Centaurus I

We present a spectroscopic analysis of Eridanus IV (Eri IV) and Centaurus I (Cen I), two ultra-faint dwarf galaxies of the Milky Way. Using IMACS/Magellan spectroscopy, we identify 28 member stars of Eri IV and 34 member stars of Cen I. For Eri IV, we measure a systemic velocity of $v_{sys} = -31.5^{+1.3}_{-1.2}\:\mathrm{km\:s^{-1}}$ and velocity dispersion $\sigma_{v}= 6.1^{+1.2}_{-0.9}\:\mathrm{km\:s^{-1}}$. Additionally, we measure the metallicities of 16 member stars of Eri IV. We find a metallicity of $\mathrm{[Fe/H]}=-2.87^{+0.08}_{-0.07}$ and resolve a dispersion of $\sigma_{\mathrm{[Fe/H]}} = 0.20\pm0.09$. The mean metallicity is marginally lower than all other known ultra-faint dwarf galaxies, making it one of the most metal-poor galaxies discovered thus far. Eri IV also has a somewhat unusual right-skewed metallicity distribution. For Cen I, we find a velocity $v_{sys} = 44.9\pm0.8\:\mathrm{km\:s^{-1}}$ and velocity dispersion $\sigma_{v} = 4.2^{+0.6}_{-0.5} \:\mathrm{km\:s^{-1}}$. We measure the metallicities of 27 member stars of Cen I, and find a mean metallicity $\mathrm{[Fe/H]} = -2.57\pm0.08$ and metallicity dispersion $\sigma_{\mathrm{[Fe/H]}} = 0.38^{+0.07}_{-0.05}$. We calculate the systemic proper motion, orbit, and the astrophysical J-factor for each system, the latter of which indicates that Eri IV is a good target for indirect dark matter detection. We also find no strong evidence for tidal stripping of Cen I or Eri IV. Overall, our measurements confirm that Eri IV and Cen I are dark matter-dominated galaxies with properties largely consistent with other known ultra-faint dwarf galaxies. The low metallicity, right-skewed metallicity distribution, and high J-factor make Eri IV an especially interesting candidate for further followup.Comment: 25 pages, 11 figures, submitted to AAS journal

Deep Photometric Observations of Ultrafaint Milky Way Satellites Centaurus I and Eridanus IV

We present deep Magellan+Megacam imaging of Centaurus I (Cen I) and Eridanus IV (Eri IV), two recently discovered Milky Way ultrafaint satellites. Our data reach ∼2–3 mag deeper than the discovery data from the DECam Local Volume Exploration Survey. We use these data to constrain their distances, structural properties (e.g., half-light radii, ellipticity, and position angle), and luminosities. We investigate whether these systems show signs of tidal disturbance and identify new potential member stars using Gaia EDR3. Our deep color–magnitude diagrams show that Cen I and Eri IV are consistent with an old (τ ∼ 13.0 Gyr) and metal-poor ([Fe/H] ≤ −2.2) stellar population. We find Cen I to have a half-light radius of rh=2.′60±0.′30 (90.6 ± 11 pc), an ellipticity of ϵ = 0.36 ± 0.05, a distance of D = 119.8 ± 4.1 kpc (m − M = 20.39 ± 0.08 mag), and an absolute magnitude of MV = −5.39 ± 0.19. Similarly, Eri IV has rh=3.′24±0.′48 (65.9 ± 10 pc), ϵ = 0.26 ± 0.09, D = 69.9 ± 3.6 kpc (m − M = 19.22 ± 0.11 mag), and MV = −3.55 ± 0.24. These systems occupy a space on the size–luminosity plane consistent with other known Milky Way dwarf galaxies, which supports the findings from our previous spectroscopic follow-up. Cen I has a well-defined morphology that lacks any clear evidence of tidal disruption, whereas Eri IV hosts a significant extended feature with multiple possible interpretations

Capillary electrophoresis of human follicular fluid

Some of the major serum proteins that are also found in follicular fluid, including transferrin, α-macroglobulin and albumin, are thought to play a role in oocyte maturation. This study set out to identify proteins in human follicular fluid by capillary zone electrophoresis and to investigate their relationship to follicular/oocyte maturity and fertility outcome. 176 individual follicular fluid samples, from 30 women undertaking in vitro fertilization/ intracytoplasmic sperm injection (IVF/ICSI), were run using an optimized capillary zone electrophoresis method that gave a good separation of sixteen peaks in most samples. Nine of the peaks were identified and quantified but seven remain unknown and require further proteomic identification. Of the identified protein peaks, levels of each were corrected for follicular volume and total content calculated. No significant difference in protein levels was found with regard to oocyte recovery and fertilization. Protein concentrations tended to decrease as the follicular sphere increased whilst total content in follicular fluid increased in proportion to size. This is consistent with simple transudation across a sphere surface area which does not increase in proportion to the follicular fluid. This is not true of the concentration and content pattern of other proteins/biomolecules which are produced by follicular cells locally. In conclusion, neither concentration nor absolute levels of nine major proteins identified in follicular fluids correlated with oocyte presence and fertility outcome. Future work to remove more concentrated proteins (e.g. albumin) would enhance separation of smaller peaks and identification of the unknown molecules