Burying the palaces? Ideologies in the Shaft Grave period

The ‘Shaft Grave Phenomenon’ is often seen as a straightforward development caused by increased access to precious (‘prestige’) items. It shall be argued here that it rather mirrors a gradual transformation of the self-identity of the mainland élites. The Shaft Grave chiefs strongly took over symbols from Crete, where the palatial élite had a strong religious connotation. This points to the conclusion that the mainland élite tried to establish a more sophisticated system of religious control, unlike the individual and personal cult visible in the MH burials. They realized the potential institutionalized religious authority had as a means of domination, connected with complex and standardised systems of representation, centralization and administration of cult, and tried to introduce this idea to the mainland. However, the symbols had to be modified and translated into a language mainland people would understand, and placed in contexts traditionally used as arenas of social display. These contexts were different from the ones the symbols originally were associated with, since on the MH mainland the grave (as opposed to the Minoan palace) was the place where transcendental ideas were articulated through material actions and expressions

Electron localization by self-assembled GaSb/GaAs quantum dots.

We have studied the photoluminescence from type-II GaSb/GaAs self-assembled quantum dots in magnetic fields up to 50 T. Our results show that at low laser power, electrons are more weakly bound to the dots than to the wetting layer, but that at high laser power, the situation is reversed. We attribute this effect to an enhanced Coulomb interaction between a single electron and dots that are multiply charged with holes

Klebsiella pneumonia strains moderately resistant to ampicillin and carbenicillin: characterization of a new β-lactamase

Klebsiella pneumoniae strain 11-03, moderately resistant to ampicillin and carbenicillin, produces one constitutive β-lactamase with an isoelectric point of 7.10 and a molecular weight of 20,000±500. The enzymatic activity is directed primarily against the penicillins, ampicillin being the best substrate. Some cephalosporins are also hydrolyzed to some extent but the affinity of the enzyme for these antibiotics is low (high Km values). It has not been possible to determine whether the biogenesis of this β-lactamase is mediated by the bacterial chromosome or by a non-transferable plasmid. This β-lactamase appears to be different, on the basis of isoelectricfocusing, from an enzyme produced by the K pneumoniae strain GN422 (Sawal et al, 1973), but similar to the latter in several propertie

Gesellschaft und Wirtschaft im archaischen Süditalien

The necropolis of Ripacandida in southern Italy (Apulia/prov. Foggia) is situated on a hill mediating between the uplands of the southern Apennines to the west and the Adriatic shore to the east. The local Archaic-Classical community (6th/5th century BCE) witnessed the rise of eastern Mediterranean settlements (apoikiai) in southern Italy and thereby the creation of a completely new situation of cultural transfer and encounter in the region. This is reflected in the material goods incorporated in the tombs that will entirely be presented and discussed in the volume. While largely maintaining their traditional ties to the neighboring areas, reflected in the use of material culture from the adjacent areas, the influx of Greek elements rises remarkably during the use period of the graveyard. It however becomes apparent that the Greek-style pottery incorporated into the local society does not replace traditional shapes and wares but rather complemented by these imports or imitations. In the course of the two centuries under investigation, the affiliation of the local community seems to shift from a more inland-orientated perspective to a closer connection to the lowlands Adriatic shore. The local community in the 6th century BCE was basically organized in an egalitarian way in small household units as suggested by the tombs arranged in clusters. In the 5th century BCE, richly equipped burials of males were separated from the tomb clusters, indicating social changes. At the same time, richly equipped females become prominent in some clusters and take over some male burial features (body placement), possibly indicating the takeover of male characteristics, like the household head, in the living community as well. The minute analysis of the local community and the comparison with other sites of the region shows that the indigenous society in the period under study is not at all static but very dynamic, and not a mere static receiver of new impulses coming from the ‘culturally superior’ (and much better researched) newcomers from the east. A number of internal social developments (i.e. hierarchization, reorganization of familial structures and gender roles, economic reorientation) occurred within the indigenous communities of southeastern Italy that cannot be attributed to external stimuli triggered by foreign presence alone

BRCA2 polymorphic stop codon K3326X and the risk of breast, prostate, and ovarian cancers

Background: The K3326X variant in BRCA2 (BRCA2*c.9976A>T; p.Lys3326*; rs11571833) has been found to be associated with small increased risks of breast cancer. However, it is not clear to what extent linkage disequilibrium with fully pathogenic mutations might account for this association. There is scant information about the effect of K3326X in other hormone-related cancers. Methods: Using weighted logistic regression, we analyzed data from the large iCOGS study including 76 637 cancer case patients and 83 796 control patients to estimate odds ratios (ORw) and 95% confidence intervals (CIs) for K3326X variant carriers in relation to breast, ovarian, and prostate cancer risks, with weights defined as probability of not having a pathogenic BRCA2 variant. Using Cox proportional hazards modeling, we also examined the associations of K3326X with breast and ovarian cancer risks among 7183 BRCA1 variant carriers. All statistical tests were two-sided. Results: The K3326X variant was associated with breast (ORw = 1.28, 95% CI = 1.17 to 1.40, P = 5.9x10- 6) and invasive ovarian cancer (ORw = 1.26, 95% CI = 1.10 to 1.43, P = 3.8x10-3). These associations were stronger for serous ovarian cancer and for estrogen receptor–negative breast cancer (ORw = 1.46, 95% CI = 1.2 to 1.70, P = 3.4x10-5 and ORw = 1.50, 95% CI = 1.28 to 1.76, P = 4.1x10-5, respectively). For BRCA1 mutation carriers, there was a statistically significant inverse association of the K3326X variant with risk of ovarian cancer (HR = 0.43, 95% CI = 0.22 to 0.84, P = .013) but no association with breast cancer. No association with prostate cancer was observed. Conclusions: Our study provides evidence that the K3326X variant is associated with risk of developing breast and ovarian cancers independent of other pathogenic variants in BRCA2. Further studies are needed to determine the biological mechanism of action responsible for these associations

At the Crossroads of Textile Cultures: Textile Production and Use at the South Italian Archaic Site of Ripacandida

Textiles are seldom included within socioeconomic interpretative frameworks of the ancient northern Mediterranean region, although several recent studies have begun to address this lacuna. The Archaic/Classical site of Ripacandida (Basilicata), located in the southern Apennines, has yielded both textiles and textile tools, providing an unprecedented opportunity to examine textile production and use at an indigenous south Italian site. This study presents the results of the complementary analyses of mineralised textile remains and textile tools (spindle whorls and loom weights) found in the cemetery of Ripacandida. The unusual combination of the Greek textile weave (weft-faced tabby) and a characteristic Italic tablet-woven border in two fragments attests to a mixed textile culture. The (to date) unique situation at Ripacandida enables us to reflect on the role of textiles in cultural contact contexts: the way in which textile cultures and their elements met and were mixed or kept separate in south Italy and beyond; the extent to which textile production was socially and economically embedded in a small indigenous community of south Italy; and the role of textiles and textile production as an expression of indigenous–Greek interaction.ER

Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus

A locus at 19p13 is associated with breast cancer (BC) and ovarian cancer (OC) risk. Here we analyse 438 SNPs in this region in 46,451 BC and 15,438 OC cases, 15,252 BRCA1 mutation carriers and 73,444 controls and identify 13 candidate causal SNPs associated with serous OC (P=9.2 × 10-20), ER-negative BC (P=1.1 × 10-13), BRCA1-associated BC (P=7.7 × 10-16) and triple negative BC (P-diff=2 × 10-5). Genotype-gene expression associations are identified for candidate target genes ANKLE1 (P=2 × 10-3) and ABHD8 (P<2 × 10-3). Chromosome conformation capture identifies interactions between four candidate SNPs and ABHD8, and luciferase assays indicate six risk alleles increased transactivation of the ADHD8 promoter. Targeted deletion of a region containing risk SNP rs56069439 in a putative enhancer induces ANKLE1 downregulation; and mRNA stability assays indicate functional effects for an ANKLE1 3′-UTR SNP. Altogether, these data suggest that multiple SNPs at 19p13 regulate ABHD8 and perhaps ANKLE1 expression, and indicate common mechanisms underlying breast and ovarian cancer risk

Quantification of silver nanoparticle uptake and distribution within individual human macrophages by FIB/SEM slice and view

Background: Quantification of nanoparticle (NP) uptake in cells or tissues is very important for safety assessment. Often, electron microscopy based approaches are used for this purpose, which allow imaging at very high resolution. However, precise quantification of NP numbers in cells and tissues remains challenging. The aim of this study was to present a novel approach, that combines precise quantification of NPs in individual cells together with high resolution imaging of their intracellular distribution based on focused ion beam/ scanning electron microscopy (FIB/SEM) slice and view approaches. Results: We quantified cellular uptake of 75 nm diameter citrate stabilized silver NPs (Ag 75 Cit) into an individual human macrophage derived from monocytic THP-1 cells using a FIB/SEM slice and view approach. Cells were treated with 10 μg/ml for 24 h. We investigated a single cell and found in total 3138 ± 722 silver NPs inside this cell. Most of the silver NPs were located in large agglomerates, only a few were found in clusters of fewer than five NPs. Furthermore, we cross-checked our results by using inductively coupled plasma mass spectrometry and could confirm the FIB/SEM results. Conclusions: Our approach based on FIB/SEM slice and view is currently the only one that allows the quantification of the absolute dose of silver NPs in individual cells and at the same time to assess their intracellular distribution at high resolution. We therefore propose to use FIB/SEM slice and view to systematically analyse the cellular uptake of various NPs as a function of size, concentration and incubation time

Die archäologischen Untersuchungen 2016–2021 in Ascoli Satriano/Giarnera Piccola (Prov. Foggia/Apulien)

Recent archaeological investigations at Ascoli Satriano have shed new light on the pre-Roman occupation of northern Apulia (ancient Daunia). The findings document a long-term and locally consistent attachment to the core site of Giarnera Piccola from the 8th to the late 4th century BCE. A close relationship is discerned, both in time and space, of architectural structures and burials. The new local discoveries testify to the existence of this situation from the earliest hut structures onwards and show its persistence until the place’s abandonment. While the detection of new fragments of two Daunian stelae in a shaft/well points to early burial activities, the character of the entangled relationship still has to be scrutinized. Archaeometric and scientific analyses furthermore attest to activities in agriculture and in pottery production