Assay of the antioxidant capacity of foods using an iron(II)-catalysed lipid peroxidation model for greater nutritional relevance

The formation of free radicals by the iron-catalysed Fenton reaction is a major cause of oxidative damage in the body. Here a common assay of antioxidant capacity, inhibition of the β-carotene-linoleic acid model of lipid peroxidation, has been modified by the addition of ferrous iron (final concentration 36 μmol/l), which makes the rate of oxidation of the lipids occur twenty-five times faster. Such an assay can simulate the oxidative damage to membrane lipids and low density lipoproteins occurring in the body in the presence of free iron. It thus may be nutritionally more relevant than traditional chemical assays of antioxidant capacity, as it measures pre-emptive antioxidant activity, i.e. activity which prevents free radicals being formed in the first place. Pre-empting their formation is likely to be more protective than scavenging of free radicals. The relative antioxidant activity of some food products found using this new assay was very different from that found using a radical-scavenging assay. Vitamin C, at 280 mg/l, was found to be sixty times better than blackcurrant puree in scavenging free radicals, but only one eighth as good as the blackcurrant puree in preventing iron-catalysed lipid peroxidation

Evaluation of exercises designed to improve children's use of descriptive words in oral language

Thesis (Ed.M.)--Boston Universit

SPIRE Map-Making Test Report

The photometer section of SPIRE is one of the key instruments on board of Herschel. Its legacy depends very much on how well the scanmap observations that it carried out during the Herschel mission can be converted to high quality maps. In order to have a comprehensive assessment on the current status of SPIRE map-making, as well as to provide guidance for future development of the SPIRE scan-map data reduction pipeline, we carried out a test campaign on SPIRE map-making. In this report, we present results of the tests in this campaign.Comment: This document has an executive summary, 6 chapters, and 102 pages. More information can be found at: https://nhscsci.ipac.caltech.edu/sc/index.php/Spire/SPIREMap-MakingTest201

Rapid submarine ice melting in the grounding zones of ice shelves in West Antarctica.

Enhanced submarine ice-shelf melting strongly controls ice loss in the Amundsen Sea embayment (ASE) of West Antarctica, but its magnitude is not well known in the critical grounding zones of the ASE's major glaciers. Here we directly quantify bottom ice losses along tens of kilometres with airborne radar sounding of the Dotson and Crosson ice shelves, which buttress the rapidly changing Smith, Pope and Kohler glaciers. Melting in the grounding zones is found to be much higher than steady-state levels, removing 300-490 m of solid ice between 2002 and 2009 beneath the retreating Smith Glacier. The vigorous, unbalanced melting supports the hypothesis that a significant increase in ocean heat influx into ASE sub-ice-shelf cavities took place in the mid-2000s. The synchronous but diverse evolutions of these glaciers illustrate how combinations of oceanography and topography modulate rapid submarine melting to hasten mass loss and glacier retreat from West Antarctica

The piranha genome provides molecular insight associated to its unique feeding behavior

The piranha enjoys notoriety due to its infamous predatory behavior but much is still not understood about its evolutionary origins and the underlying molecular mechanisms for its unusual feeding biology. We sequenced and assembled the red-bellied piranha (Pygocentrus nattereri) genome to aid future phenotypic and genetic investigations. The assembled draft genome is similar to other related fishes in repeat composition and gene count. Our evaluation of genes under positive selection suggests candidates for adaptations of piranhas’ feeding behavior in neural functions, behavior, and regulation of energy metabolism. In the fasted brain, we find genes differentially expressed that are involved in lipid metabolism and appetite regulation as well as genes that may control the aggression/boldness behavior of hungry piranhas. Our first analysis of the piranha genome offers new insight and resources for the study of piranha biology and for feeding motivation and starvation in other organisms

Seasonal and Spatial Dynamics of Alate Aphid Dispersal in Snap Bean Fields in Proximity to Alfalfa and Implications for Virus Management

Alfalfa is a source for viruses that may be acquired by aphids and transmitted to snap bean, Phaseolus vulgaris L. Snap bean fields in proximity to alfalfa could have an increased risk of virus infection. Knowledge of the abundance and temporal and spatial dispersal patterns of commonly encountered aphids in commercial snap bean fields, varying in distance from alfalfa, could provide insight into this risk. Alate aphids were monitored using water pan traps in snap bean and alfalfa fields that were adjacent to or >1 km away from each other. The pea aphid, Acyrthosiphon pisum (Harris), was the most common aphid species captured in early-planted snap bean fields in 2002 and 2003 (56 and 23% of total, respectively), whereas the corn leaf aphid, Rhopalosiphum maidis (Fitch), also was common in 2003 (15% of total). In contrast, the yellow clover aphid, Therioaphis trifolii (Monell), and soybean aphid, Aphis glycines Matsumura, were the most abundant species trapped in late-planted snap bean fields in 2002 (77% of total) and 2003 (64% of total), respectively. These species were prevalent in traps in alfalfa as well. The abundance and temporal dispersal patterns of these species in snap beans adjacent to and >1 km away from alfalfa were similar, suggesting that the risk for virus infection may not be affected by proximity to alfalfa. A similar number of alate aphids also were captured along snap bean field edges and field centers, regardless of their proximity to alfalfa. This suggests that the aphids dispersed into snap bean randomly rather than directionally from the field edge. The implication of these results is that separating snap bean fields from alfalfa or using crop borders/barriers are not likely to be successful virus management strategie

Toll-like receptor 4 mediates synergism between alcohol and HCV in hepatic oncogenesis involving stem cell marker Nanog

Alcohol synergistically enhances the progression of liver disease and the risk for liver cancer caused by hepatitis C virus (HCV). However, the molecular mechanism of this synergy remains unclear. Here, we provide the first evidence that Toll-like receptor 4 (TLR4) is induced by hepatocyte-specific transgenic (Tg) expression of the HCV nonstructural protein NS5A, and this induction mediates synergistic liver damage and tumor formation by alcohol-induced endotoxemia. We also identify Nanog, the stem/progenitor cell marker, as a novel downstream gene up-regulated by TLR4 activation and the presence of CD133/Nanog-positive cells in liver tumors of alcohol-fed NS5A Tg mice. Transplantation of p53-deficient hepatic progenitor cells transduced with TLR4 results in liver tumor development in mice following repetitive LPS injection, but concomitant transduction of Nanog short-hairpin RNA abrogates this outcome. Taken together, our study demonstrates a TLR4-dependent mechanism of synergistic liver disease by HCV and alcohol and an obligatory role for Nanog, a TLR4 downstream gene, in HCV-induced liver oncogenesis enhanced by alcohol

The Central Engines of 19 LINERs as Viewed by Chandra

Using archival Chandra observations of 19 LINERs we explore the X-ray properties of their inner kiloparsec to determine the origin of their nuclear X-ray emission, to investigate the presence of an AGN, and to identify the power source of the optical emission lines. The relative numbers of LINER types in our sample are similar to those in optical spectroscopic surveys. We find that diffuse, thermal emission is very common and is concentrated within the central few hundred parsec. The average spectra of the hot gas in spirals and ellipticals are very similar to those of normal galaxies. They can be fitted with a thermal plasma (kT~0.5 keV) plus a power law (photon index of 1.3-1.5) model. There are on average 3 detected point sources in their inner kiloparsec with L(0.5-10 keV)~10^37-10^40 erg/s. The average cumulative luminosity functions for sources in spirals and ellipticals are identical to those of normal galaxies. In the innermost circle of 2.5" radius in each galaxy we find an AGN in 12 of the 19 galaxies. The AGNs contribute a median of 60% of the 0.5-10 keV luminosity of the central 2.5" region, they have luminosities of 10^37-10^39 erg/s (Eddington ratios 10^-8 to 10^-5). The ionizing luminosity of the AGNs is not enough to power the observed optical emission lines in this particular sample. Thus, we suggest that the lines are powered either by the mechanical interaction of an AGN jet (or wind) with the circumnuclear gas, or by stellar processes, e.g. photoionization by post-AGB stars or young stars.Comment: Accepted by Ap.J. 23 pages, 8 figures, emulatepj format, images of fig 1 not included, for complete PDF preprint see http://www.astro.psu.edu/users/mce/preprints

Crustal thickness and Moho character of the fast-spreading East Pacific Rise from 9°42′N to 9°57′N from poststack-migrated 3-D MCS data

Author Posting. © American Geophysical Union, 2014. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geochemistry, Geophysics, Geosystems 15 (2014): 634–657, doi:10.1002/2013GC005069.We computed crustal thickness (5740 ± 270 m) and mapped Moho reflection character using 3-D seismic data covering 658 km2 of the fast-spreading East Pacific Rise (EPR) from 9°42′N to 9°57′N. Moho reflections are imaged within ∼87% of the study area. Average crustal thickness varies little between large sections of the study area suggesting regionally uniform crustal production in the last ∼180 Ka. However, individual crustal thickness measurements differ by as much as 1.75 km indicating that the mantle melt delivery has not been uniform. Third-order, but not fourth-order ridge discontinuities are associated with changes in the Moho reflection character and/or near-axis crustal thickness. This suggests that the third-order segmentation is governed by melt distribution processes within the uppermost mantle while the fourth-order ridge segmentation arises from midcrustal to upper-crustal processes. In this light, we assign fourth-order ridge discontinuity status to the debated ridge segment boundary at ∼9°45′N and third-order status at ∼9°51.5′N to the ridge segment boundary previously interpreted as a fourth-order discontinuity. Our seismic results also suggest that the mechanism of lower-crustal accretion varies along the investigated section of the EPR but that the volume of melt delivered to the crust is mostly uniform. More efficient mantle melt extraction is inferred within the southern half of our survey area with greater proportion of the lower crust accreted from the axial magma lens than that for the northern half. This south-to-north variation in the crustal accretion style may be caused by interaction between the melt sources for the ridge and the Lamont seamounts.This research was supported by the National Science Foundation grants OCE0327872 to J. C. M., S. M. C., OCE327885 to J. P. C., OCE0624401 to M. R. N., and NSERC Discovery, CRC and CFI grants to M. R. N.2014-09-1