Evaluating genetic traceability methods for captive bred marine fish and their applications in fisheries management and wildlife forensics

Growing demands for marine fish products is leading to increased pressure on already depleted wild populations and a rise in the aquaculture production. Consequently, more captive bred fish are released into the wild through accidental escape or deliberate restocking, stock enhancement and sea ranching programs. The increased mixing of captive bred fish with wild conspecifics may affect the ecological and/or genetic integrity of wild fish populations. From a fisheries management perspective unambiguous identification tools for captive bred fish will be highly valuable to manage risks. Additionally there is great potential to use these tools in wildlife forensics (i.e. tracing back escapees to their origin and determining mislabelling of seafood products). Using SNP data from captive bred and wild populations of Atlantic cod (Gadus morhua L.) and sole (Solea solea L.), we explored the efficiency of population and parentage assignment techniques for the identification and tracing of captive bred fish. Simulated and empirical data were used to correct for stochastic genetic effects. Overall, parentage assignment performed well when a large effective population size characterizes the broodstock and escapees originate from early generations of captive breeding. Consequently, parentage assignments are particularly useful from a fisheries management perspective to monitor the effects of deliberate releases of captive bred fish on wild populations. Population assignment proved to be more efficient after several generations of captive breeding, which makes it a useful method in forensic applications for well-established aquaculture species. We suggest the implementation of a case by case strategy when choosing the best method

Outlier Loci Detect Intraspecific Biodiversity amongst Spring and Autumn Spawning Herring across Local Scales

Herring, Clupea harengus, is one of the ecologically and commercially most important species in European northern seas, where two distinct ecotypes have been described based on spawning time; spring and autumn. To date, it is unknown if these spring and autumn spawning herring constitute genetically distinct units. We assessed levels of genetic divergence between spring and autumn spawning herring in the Baltic Sea using two types of DNA markers, microsatellites and Single Nucleotide Polymorphisms, and compared the results with data for autumn spawning North Sea herring. Temporally replicated analyses reveal clear genetic differences between ecotypes and hence support reproductive isolation. Loci showing non-neutral behaviour, so-called outlier loci, show convergence between autumn spawning herring from demographically disjoint populations, potentially reflecting selective processes associated with autumn spawning ecotypes. The abundance and exploitation of the two ecotypes have varied strongly over space and time in the Baltic Sea, where autumn spawners have faced strong depression for decades. The results therefore have practical implications by highlighting the need for specific management of these co-occurring ecotypes to meet requirements for sustainable exploitation and ensure optimal livelihood for coastal communitie

“Gladly given for the cause”: New Brunswick Teacher and Student Support for the War Effort, 1914–1918

This article examines the efforts of New Brunswick’s public school teachers and students to support the First World War. Along with participation in the Teachers’ Machine Gun Fund, they were involved in gendered activities such as Home Efficiency Clubs, Cadet Corps, Military Drill, and the “Soldiers of the Soil” movement. Students—male and female, young and old, Anglophone and Francophone—as well as their teachers, also took part in a variety of fundraising efforts that served to reinforce discipline and inculcate patriotism, meeting the state-sponsored definition of a good citizen. By drawing on archival documents, this article highlights the ways that New Brunswick’s teachers and students were inculcated in voluntarism, patriotism, and militarism as they responded to the demands of the war and contributed “for the cause.”Résumé Cet article fait le bilan des efforts des enseignants et des élèves des écoles publiques du Nouveau-Brunswick pour appuyer l’effort de la Première Guerre mondiale. Grâce à la participation du Fonds de la Mitrailleuse des Enseignants, ils ont participé à des activités intégrées, telles que les Clubs d’efficacité au foyer, les Corps de cadets, les Corvées de militaires et le mouvement des « Soldats de la terre ». Des élèves, garçons et filles, jeunes et moins jeunes, francophones et anglophones, ainsi que leurs enseignants, ont également participé à diverses campagnes de financement qui ont servi à renforcer la discipline, à inculquer le patriotisme et à promouvoir la définition d’état de bon citoyen. En se fondant sur des documents d’archives, cet article souligne les moyens par lesquels les enseignants et les étudiants du Nouveau-Brunswick ont embrassé le bénévolat, le patriotisme et le militantisme quand ils ont répondu aux besoins de l’effort de guerre et ont contribué « à la cause ».Cet article fait le bilan des efforts des enseignants et des élèves des écoles publiques du Nouveau-Brunswick pour appuyer l’effort de la Première Guerre mondiale. Grâce à la participation du Fonds de la Mitrailleuse des Enseignants, ils ont participé à des activités intégrées, telles que les Clubs d’efficacité au foyer, les Corps de cadets, les Corvées de militaires et le mouvement des « Soldats de la terre ». Des élèves, garçons et filles, jeunes et moins jeunes, francophones et anglophones, ainsi que leurs enseignants, ont également participé à diverses campagnes de financement qui ont servi à renforcer la discipline, à inculquer le patriotisme et à promouvoir la définition d’état de bon citoyen. En se fondant sur des documents d’archives, cet article souligne les moyens par lesquels les enseignants et les étudiants du Nouveau-Brunswick ont embrassé le bénévolat, le patriotisme et le militantisme quand ils ont répondu aux besoins de l’effort de guerre et ont contribué « à la cause »

Genetic homogeneity in the deep-sea grenadier Macrourus berglax across the North Atlantic Ocean

Paucity of data on population structure and connectivity in deep sea species remains a major obstacle to their sustainable management and conservation in the face of ever increasing fisheries pressure and other forms of impacts on deep sea ecosystems. The roughhead grenadier Macrourus berglax presents all the classical characteristics of a deep sea species, such as slow growth and low fecundity, which make them particularly vulnerable to anthropogenic impact, due to their low resilience to change. In this study, the population structure of the roughhead grenadier is investigated throughout its geographic distribution using two sets of molecular markers: a partial sequence of the Control Region of mitochondrial DNA and species-specific microsatellites. No evidence of significant structure was found throughout the North Atlantic, with both sets of molecular markers yielding the same results of overall homogeneity. We posit two non-mutually exclusive scenarios that can explain such outcome: i) substantial high gene flow among locations, possibly maintained by larval stages, ii) very large effective size of post-glacially expanded populations. The results can inform management strategies in this by-caught species, and contribute to the broader issue of biological connectivity in the deep ocean

Competitive Boosterism: How Milwaukee Lost the Braves

By any measure, major-league baseball in North America surely qualifies as big business. The national pastime is a vital component of today\u27s urban political economy, and baseball teams resemble other high-prestige businesses in that cities must compete for the privilege of hosting them - whatever their true worth. A study analyzes the transfer of the Milwaukee Braves baseball franchise to Atlanta in 1965 as the outcome of competitive boosterism or the active participation of local elites in luring trade, industry, and investment from other cities for the purpose of economic development

Images of Moby-Dick: An exhibition of editions of Herman Melville's great novel of whaling

This publication was made possible by a grant from The National Endowment for the Humanities, a federal agency

Civil, Canon and Common: Aspects of Legal History

An exhibition of books and manuscripts in the Kenneth Spencer Research Librar

Coalescent-based species delimitation in the sand lizards of the Liolaemus wiegmannii complex (Squamata: Liolaemidae)

Coalescent-based algorithms coupled with the access to genome-wide data have become powerful tools forassessing questions on recent or rapid diversification, as well as delineating species boundaries in the absence of reciprocal monophyly. In southern South America, the diversification of Liolaemus lizards during the Pleistocene is well documented and has been attributed to the climatic changes that characterized this recent period of time. Past climatic changes had harsh effects at extreme latitudes, including Patagonia, but habitat changes at intermediate latitudes of South America have also been recorded, including expansion of sand fields over northern Patagonia and Pampas). In this work, we apply a coalescent-based approach to study the diversification of the Liolaemus wiegmannii species complex, a morphologically conservative clade that inhabits sandy soils across northwest and south-central Argentina, and the south shores of Uruguay. Using four standard sequence markers (mitochondrial DNA and three nuclear loci) along with ddRADseq data we inferred species limits and a time calibrated species tree for the L. wiegmannii complex in order to evaluate the influence of Quaternary sand expansion/retraction cycles on diversification. We also evaluated the evolutionary independence of the recently described L. gardeli and inferred its phylogenetic position relative to L. wiegmannii. We find strong evidence for six allopatric candidate species within L. wiegmannii, which diversified during the Pleistocene. The Great Patagonian Glaciation (∼1 million years before present) likely split the species complex into two main groups: one composed of lineages associated with sub-Andean sedimentary formations, and the other mostly related to sand fields in the Pampas and northern Patagonia. We hypothesize that early speciation within L. wiegmannii was influenced by the expansion of sand dunes throughout central Argentina and Pampas. Finally, L. gardeli is supported as a distinct lineage nested within the L. wiegmannii complex.Fil: Villamil, Joaquín. Universidad de la República. Facultad de Ciencias; UruguayFil: Avila, Luciano Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Morando, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto Patagónico para el Estudio de los Ecosistemas Continentales; ArgentinaFil: Sites, Jack W.. University Brigham Young; Estados UnidosFil: Leaché, Adam D.. University of Washington; Estados UnidosFil: Maneyro, Raúl. Universidad de la República. Facultad de Ciencias; UruguayFil: Camargo Bentaberry, Arley. Universidad de la República; Urugua

SNP discovery using next generation transcriptomic sequencing in Atlantic herring (Clupea harengus)

The introduction of Next Generation Sequencing (NGS) has revolutionised population genetics, providing studies of non-model species with unprecedented genomic coverage, allowing evolutionary biologists to address questions previously far beyond the reach of available resources. Furthermore, the simple mutation model of Single Nucleotide Polymorphisms (SNPs) permits cost-effective high-throughput genotyping in thousands of individuals simultaneously. Genomic resources are scarce for the Atlantic herring (Clupea harengus), a small pelagic species that sustains high revenue fisheries. This paper details the development of 578 SNPs using a combined NGS and high-throughput genotyping approach. Eight individuals covering the species distribution in the eastern Atlantic were bar-coded and multiplexed into a single cDNA library and sequenced using the 454 GS FLX platform. SNP discovery was performed by de novo sequence clustering and contig assembly, followed by the mapping of reads against consensus contig sequences. Selection of candidate SNPs for genotyping was conducted using an in silico approach. SNP validation and genotyping were performed simultaneously using an Illumina 1,536 GoldenGate assay. Although the conversion rate of candidate SNPs in the genotyping assay cannot be predicted in advance, this approach has the potential to maximise cost and time efficiencies by avoiding expensive and time-consuming laboratory stages of SNP validation. Additionally, the in silico approach leads to lower ascertainment bias in the resulting SNP panel as marker selection is based only on the ability to design primers and the predicted presence of intron-exon boundaries. Consequently SNPs with a wider spectrum of minor allele frequencies (MAFs) will be genotyped in the final panel. The genomic resources presented here represent a valuable multi-purpose resource for developing informative marker panels for population discrimination, microarray development and for population genomic studies in the wild

Speciation of phosphorus in a fertilized, reduced-till soil system: in-field treatment incubation study

Citation: Khatiwada, Raju, Ganga M. Hettiarachchi, David B. Mengel, and Mingwei Fei. “Speciation of Phosphorus in a Fertilized, Reduced-Till Soil System: In-Field Treatment Incubation Study.” Soil Science Society of America Journal 76, no. 6 (2012): 2006–18. https://doi.org/10.2136/sssaj2011.0299.Phosphorus management in reduced-tillage systems is a great concern for farmers. Conclusive positive results of deep-banding P fertilizers compared with broadcast application and the chemistry of reduced-tillage systems remain unclear. Knowledge of the dominant solid P species present in soil following application of P fertilizers and the resulting potential P availability would help us understand and efficiently manage P in reduced-tillage systems. The objective of this research was to study the influence of placement (broadcast vs. deep-band P), fertilizer source (granular vs. liquid P), and time on the reaction products of P under field conditions. Changes in soil pH, resin-extractable P, total P, and speciation of P were determined at different distances from the point of fertilizer application at 5 wk and 6 mo after P application at a rate of 75 kg ha−1 to a soil system that was under long-term reduced tillage. Resin-extractable P was lower for broadcast treatments compared with deep-band treatments for both time periods. Resin-extractable P was greater in the liquid P-treated soils than in the granular P-treated soils. Speciation results showed that granular P fertilizers tended to form Fe–P-like forms, whereas liquid forms remained in adsorbed P-like forms in the soil 5 wk after application; moreover, speciation results showed granular P fertilizers precipitated less when deep-banded. During the 6-mo period following application, reaction products of broadcast granular, broadcast liquid, and deep-band granular fertilizers transformed to Ca-phosphate or mixtures of Ca-, Fe- and adsorbed-phosphate-like forms, whereas deep-band liquid P remained as mainly adsorbed P-like forms. Deep-banding of P would most likely provide a solution that is both agronomically and environmentally efficient for reduced-till farmers