Forage legume silage and cold-pressed rapeseed cake for dairy bull calves

Rearing of organic dairy bull calves can be contradictory, using calves with a high need for quality protein at the same time as a high intake of forage (>60% up to 6 months of age) is required. Forage legumes contain more crude protein than grass, but with high rumen degradability of the protein. When legume forage is fed together with energy-rich cold-pressed rapeseed cake (CRC) the protein in the feeds can be utilized to a higher extent and a satisfactory calf weight gain can be achieved as shown. Using locally produced protein feeds instead of the often used soya bean meal (SBM) is of great interest not only in organic but also in conventional feeding because of environmentally advantages

USING CLOVER/GRASS SILAGE AS A PROTEIN FEED FOR DAIRY BULL CALVES

Calves need high concentrations of protein with high protein quality in their feed ration for proper growth. Soya bean meal is widely used in the world as a protein feed of good nutritional quality but the ethics around the cultivation of the beans are often questioned. Home grown or locally produced feeds, such as nitrogen fixating forage and grain legumes as well as rapeseed products, are shown to give less contribution to environmental problems than imported feeds. The objective of the study was to compare dry matter intake, live weight gain, feed efficiency and profitability in calves fed two levels of forages with high inclusion of red clover together with two different amounts of rapeseed cake vs. soya bean meal. Soya bean meal gave the highest live weight gain and the best profitability. However, a greater amount of rapeseed together with clover/grass silage also gave a good live weight gain and profitability, close to the results for soya bean meal

Influence of slaughter age and carcass suspension on meat quality in Angus heifers

This study investigated the effects of pelvic suspension and slaughter age on longissimus thoracis et lumborum (LTL) from 40 heifers with at least 75% Angus breeding. A total of 20 heifers were slaughtered directly from pasture at 18 months of age, and carcass sides were hung either by the Achilles tendon or the pelvic bone. The other 20 heifers were assigned to an additional winter housing period and slaughtered at 22 months of age; carcass sides were hung only by Achilles suspension. All carcasses were electrically stimulated and assessed according to the EUROP carcass classification system. In addition, the LTL muscles were aged for 7 or 14 days before meat quality was evaluated for intramuscular fat (IMF), drip loss, colour, shear force, compression and sensory analysis. The 22-month-old heifers were heavier, fatter and had more IMF than 18-month-old heifers. Conformation scores (muscling) did not differ between the two slaughter groups. Pelvic suspension reduced both between- and within-animal variation for peak force, total energy and compression peak force. For the 18-month-old heifers, pelvic suspension also decreased peak force, total energy and compression variables for the LTL muscles from both ageing periods, whereas Achilles-suspended samples had lower shear force values only at day 14. Sensory analysis showed that pelvic-suspended sides had greater tenderness, lower bite resistance, less threadiness, higher juiciness and meat flavour and less visible marbling than meat from Achilles-suspended sides. Pelvic-suspended sides at 18 months of age were similar in peak force and total energy values to the 22-month-old heifers. The importance of ageing the Achilles-suspended sides was more obvious for samples from 18-month-old heifers than from the 22-month-old animals. The correlations between the different instrumental measurements and sensory tenderness were considerably higher for carcasses suspended by the Achilles tendon (r = -520.55 to 0.20) than for those hung by the pelvic bone (r = -520.25 to 0.19). More correlations between sensory-evaluated tenderness and shear variables were significant after 7 days (n = 6) of ageing than after 14 days (n = 4) of ageing. This study clearly shows the benefits of pelvic suspension, which reduces the need for additional feeding after pasture

Splice-site mutations cause Rrp6-mediated nuclear retention of the unspliced RNAs and transcriptional down-regulation of the splicing-defective genes

Background: Eukaryotic cells have developed surveillance mechanisms to prevent the expression of aberrant transcripts. An early surveillance checkpoint acts at the transcription site and prevents the release of mRNAs that carry processing defects. The exosome subunit Rrp6 is required for this checkpoint in Saccharomyces cerevisiae, but it is not known whether Rrp6 also plays a role in mRNA surveillance in higher eukaryotes. Methodology/Principal Findings: We have developed an in vivo system to study nuclear mRNA surveillance in Drosophila melanogaster. We have produced S2 cells that express a human b-globin gene with mutated splice sites in intron 2 (mut bglobin). The transcripts encoded by the mut b-globin gene are normally spliced at intron 1 but retain intron 2. The levels of the mut b-globin transcripts are much lower than those of wild type (wt) ß-globin mRNAs transcribed from the same promoter. We have compared the expression of the mut and wt b-globin genes to investigate the mechanisms that downregulate the production of defective mRNAs. Both wt and mut b-globin transcripts are processed at the 39, but the mut bglobin transcripts are less efficiently cleaved than the wt transcripts. Moreover, the mut b-globin transcripts are less efficiently released from the transcription site, as shown by FISH, and this defect is restored by depletion of Rrp6 by RNAi. Furthermore, transcription of the mut b-globin gene is significantly impaired as revealed by ChIP experiments that measure the association of the RNA polymerase II with the transcribed genes. We have also shown that the mut b-globin gene shows reduced levels of H3K4me3. Conclusions/Significance: Our results show that there are at least two surveillance responses that operate cotranscriptionally in insect cells and probably in all metazoans. One response requires Rrp6 and results in the inefficient release of defective mRNAs from the transcription site. The other response acts at the transcription level and reduces the synthesis of the defective transcripts through a mechanism that involves histone modifications

Forest cows secrets: Cracking the code with movement sensors

Have you ever wondered how we can watch animals in the wild without actually being near them? In Norway, cows roam freely in the deep forest during summer. While the cows enjoy the freedom, it can be tricky to keep them safe from carnivores like wolves and bears, as no shepherds or dogs protect the herds. Keeping an eye on the cows is important! Farmers and researchers use GPS to track animals, just as we do for phones or cars. However, GPS does not tell us much about what the animals are doing. That is where movement sensors come in. These sensors store information about the tiniest body movements and reveal what the animal is doing at any time. Is the cow’s head up or down? Is it walking or running? Based on the data, we could distinguish 20 different behaviors! Now we can spy on cows, see what they are up to in the forest, and help farmers better care for them

Pbx loss in cranial neural crest, unlike in epithelium, results in cleft palate only and a broader midface.

Orofacial clefting represents the most common craniofacial birth defect. Cleft lip with or without cleft palate (CL/P) is genetically distinct from cleft palate only (CPO). Numerous transcription factors (TFs) regulate normal development of the midface, comprising the premaxilla, maxilla and palatine bones, through control of basic cellular behaviors. Within the Pbx family of genes encoding Three Amino-acid Loop Extension (TALE) homeodomain-containing TFs, we previously established that in the mouse, Pbx1 plays a preeminent role in midfacial morphogenesis, and Pbx2 and Pbx3 execute collaborative functions in domains of coexpression. We also reported that Pbx1 loss from cephalic epithelial domains, on a Pbx2- or Pbx3-deficient background, results in CL/P via disruption of a regulatory network that controls apoptosis at the seam of frontonasal and maxillary process fusion. Conversely, Pbx1 loss in cranial neural crest cell (CNCC)-derived mesenchyme on a Pbx2-deficient background results in CPO, a phenotype not yet characterized. In this study, we provide in-depth analysis of PBX1 and PBX2 protein localization from early stages of midfacial morphogenesis throughout development of the secondary palate. We further establish CNCC-specific roles of PBX TFs and describe the developmental abnormalities resulting from their loss in the murine embryonic secondary palate. Additionally, we compare and contrast the phenotypes arising from PBX1 loss in CNCC with those caused by its loss in the epithelium and show that CNCC-specific Pbx1 deletion affects only later secondary palate morphogenesis. Moreover, CNCC mutants exhibit perturbed rostro-caudal organization and broadening of the midfacial complex. Proliferation defects are pronounced in CNCC mutants at gestational day (E)12.5, suggesting altered proliferation of mutant palatal progenitor cells, consistent with roles of PBX factors in maintaining progenitor cell state. Although the craniofacial skeletal abnormalities in CNCC mutants do not result from overt patterning defects, osteogenesis is delayed, underscoring a critical role of PBX factors in CNCC morphogenesis and differentiation. Overall, the characterization of tissue-specific Pbx loss-of-function mouse models with orofacial clefting establishes these strains as unique tools to further dissect the complexities of this congenital craniofacial malformation. This study closely links PBX TALE homeodomain proteins to the variation in maxillary shape and size that occurs in pathological settings and during evolution of midfacial morphology

Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation

Previous studies have shown that exogenous ATP (>1µM) prevents bone formation in vitro by blocking mineralisation of the collagenous matrix. This effect is thought to be mediated via both P2 receptor-dependent pathways and a receptor-independent mechanism (hydrolysis of ATP to produce the mineralisation inhibitor pyrophosphate, PPi). Osteoblasts are also known to release ATP constitutively. To determine whether this endogenous ATP might exert significant biological effects, bone-forming primary rat osteoblasts were cultured with 0.5-2.5U/ml apyrase (which sequentially hydrolyses ATP to ADP to AMP + 2Pi). Addition of 0.5U/ml apyrase to osteoblast culture medium degraded extracellular ATP to <1% of control levels within 2 minutes; continuous exposure to apyrase maintained this inhibition for up to 14 days. Apyrase treatment for the first 72 hours of culture caused small decreases (≤25%) in osteoblast number, suggesting a role for endogenous ATP in stimulating cell proliferation. Continuous apyrase treatment for 14 days (≥0.5U/ml) increased mineralisation of bone nodules by up to 3-fold. Increases in bone mineralisation were also seen when osteoblasts were cultured with the ATP release inhibitors, NEM and brefeldin A, as well as with P2X1 and P2X7 receptor antagonists. Apyrase decreased alkaline phosphatase (TNAP) activity by up to 60%, whilst increasing the activity of the PPi-generating ecto-nucleotide pyrophosphatase/phosphodiesterases (NPPs) up to 2.7-fold. Both collagen production and adipocyte formation were unaffected. These data suggest that nucleotides released by osteoblasts in bone could act locally, via multiple mechanisms, to limit mineralisation

Carcass characteristics of Nordic native cattle breeds

Native livestock breeds are part of the history of the Nordic people and comprise a resource for future foodproduction. In this study, net gain and carcass characteristics of two Danish, three Finnish, one Icelandic, six Norwegian andfive Swedish native cattle breeds were retrieved and compared to commercial breeds: two beef breeds and two dairy breeds.Breed data were collected from national databases and sorted into six animal categories: young bull, bull, steer, heifer, youngcow and cow, for which means and standard deviations were calculated within each country. The native breeds ranged fromsmall-sized milking type breeds with low net gain, carcass weights and EUROP classification to larger multipurpose breedswith high net gains, carcass weights and EUROP classification.All Finnish and most of the Norwegian and Swedish native breeds had lower net gain and carcass weight than the dairy breedsin the same category and country, but with similar carcass conformation and fatness scores. The two Danish native breedshad higher net gain, carcass weight and conformation class than the reference dairy breed, but lower than the reference beefbreeds. The net gain and carcass traits of the Icelandic native breed were similar to the smallest-sized native breeds fromthe other countries. The carcass traits of the native breeds indicate that they have comparative advantages in an extensiveproduction system based on forage and marginal grasslands. They may also succeed better in the value-added markets thanin mainstream beef production

The Decline and Possible Return of Silvipastoral Agroforestry in Sweden

Silvipastoral agroforestry in the form of forest grazing and wooded semi-natural pastures has historically been very important for the Swedish supply of food and wood products for local use. Since the end of the 1800s, this form of combined production system has greatly decreased and now covers only 1% of Sweden's land area. However, in recent decades it has gained increased relevance for reasons of landscape, biodiversity and climate. Agroforestry's decline and possible future increase are described through reviews of statistics and the literature read by farmers and politicians whose decisions are behind the development. Especially when it comes to biodiversity and climate, this review also includes the scientific literature. Surveys on Swedish citizens' valuation of silvipastoral agroforestry landscapes compared to treeless pasture and closed forest are also reviewed. It is possible that efforts to increase Sweden's low self-sufficiency in beef and lamb meat, the coming requirements according to the EU's nature restoration law and the need to limit climate change through carbon sequestration in trees may again increase the area of silvipastoral agroforestry. For this to be economically feasible, large grazing areas can be created out of remaining small scattered wooded semi-natural pastures and intervening forestland, which historically may have been grazed forests

Economic Comparison between Pasture-Based Beef Production and Afforestation of Abandoned Land in Swedish Forest Districts

Large areas of agricultural land have been abandoned or are at risk of being abandoned such as small scattered fields and pastures in forest-dominated landscapes are unsuitable for modern mechanized agriculture and cost-efficient grazing. These areas have therefore become unprofitable to cultivate and graze. Spruce planting has been seen as the obvious alternative on these lands but is today questioned from landscape points of view. Now most abandoned land is left for natural afforestation. This study aims to compare the profitability in use of abandoned or marginal agricultural land in Swedish forest districts for spruce planting, natural birch afforestation, or organic beef cattle grazing large pasture-forest mosaics. The pastures consist of remaining semi-natural pastures, abandoned and marginal agricultural land, and adjacent forest land. Calculations of contribution to land, management, and risk suggest that, given present supports and environmental payments, organic beef production with herds of more than 20 suckler cows in large pasture-forest mosaics could be more profitable than forestry, except for in the most fertile areas of southern Sweden, where spruce planting has the highest contribution. Future tree breeding progress and possible decrease of livestock-related support and environmental payments would however increase the competitiveness of resumed afforestation relative to beef production