Hyperspectral discrimination of tree species with different classifications using single- and multiple-endmember.

Discrimination of tree species with different ages is performed in three classifications using hyperspectral data. The first classification is between Broadleaves and pines; the second classification is between Broadleaves, Corsican Pines, and Scots Pines, and the third classification is between six tree species including different ages of Corsican and Scots Pines. These three classifications are performed by having single- and multiple-endmember and considering five different spectral measure techniques (SMTs) in combination with reflectance spectra (ReflS), first and second derivative spectra. The result shows that using single-endmember, derivative spectra are not useful for a more challenging classification. This is further emphasized in multiple-endmember classification, where all SMTs perform better in ReflS rather than derivative in all classifications. Furthermore, using derivative spectra, discrimination accuracy become more dependent on the type of SMTs, especially in single-endmember. By employing multiple-endmember, the within-species variation is significantly reduced, thereby, the remaining challenge in discriminating tree species with different ages is only due to the between-species similarity. Overall, discrimination accuracies around 92.4, 76.8, and 71.5% are obtained using original reflectance and multiple-endmember for the first, second, and third classification, which is around 14.3, 17, and 8.3% higher than what were obtained in single-endmember classifications, respectively. Also, amongst the five SMTs, Euclidean distance (in both single- and multiple-endmember) and Jeffreys–Matusita distance (in single-endmember and derivative spectra) provided the highest discrimination accuracies in different classifications. Furthermore, when discrimination become more challenging from the first to second and third classification, the performance difference between different SMTs is increased from 1.4 to 3.8 and 7.3%, respectively. The study shows high potential of multiple-endmember to be employed in remote sensing applications in the future for improving tree species discrimination accuracy

Nuclear magnetic resonance: a tool for imaging belowground damage caused by Heterodera schachtii and Rhizoctonia solani on sugar beet

Belowground symptoms of sugar beet caused by the beet cyst nematode (BCN) Heterodera schachtii include the development of compensatory secondary roots and beet deformity, which, thus far, could only be assessed by destructively removing the entire root systems from the soil. Similarly, the symptoms of Rhizoctonia crown and root rot (RCRR) caused by infections of the soil-borne basidiomycete Rhizoctonia solani require the same invasive approach for identification. Here nuclear magnetic resonance imaging (MRI) was used for the non-invasive detection of belowground symptoms caused by BCN and/or RCRR on sugar beet. Excessive lateral root development and beet deformation of plants infected by BCN was obvious 28 days after inoculation (dai) on MRI images when compared with non-infected plants. Three-dimensional images recorded at 56 dai showed BCN cysts attached to the roots in the soil. RCRR was visualized by a lower intensity of the MRI signal at sites where rotting occurred. The disease complex of both organisms together resulted in RCRR development at the site of nematode penetration. Damage analysis of sugar beet plants inoculated with both pathogens indicated a synergistic relationship, which may result from direct and indirect interactions. Nuclear MRI of plants may provide valuable, new insight into the development of pathogens infecting plants below- and aboveground because of its non-destructive nature and the sufficiently high spatial resolution of the method

Beauveria bassiana as an endophyte in tissue cultured banana plants: a novel way to combat the banana weevil Cosmopolites sordidus

Beauveria bassiana, which is effective against a range of insect pests, is the most researched and commercialised fungal biopesticide. Laboratory and screenhouse studies have revealed that this entomopathogenic fungus has great potential for use against the banana weevil, Cosmopolites sordidus. However, impractical field delivery methods and high costs associated with the application of B. bassiana prevent its use and commercialisation in banana fields against C. sordidus. Our research has revealed that B. bassiana can colonise internal banana tissues for at least 4 months after tissue-cultured plantlets are dipped in a spore suspension. The type of banana cultivar did not affect colonisation by B. bassiana, and, even when elevating doses, plant growth was not reduced. In a set of three screenhouse experiments, larval mortality rates in B. bassiana-treated plants were 24-89%, and the presence of the fungus led to a reduction in larval damage of >50%. Application of B. bassiana as an endophyte could circumvent bottlenecks associated with its application as a conventional biopesticide

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Plant-parasitic nematodes of potential phytosanitary importance, their main hosts and reported yield losses

The potential phytosanitary importance of all named plant-parasitic nematode species was determined by evaluating available information on species characteristics, association with economically-important crop hosts, and ability to act as vectors of viruses or form disease complexes with other pathogens. Most named species of plant-parasitic nematodes (PPN) are poorly known, recorded from a single location only, not associated with economically-important crops, and not known to be associated with other plant disease organisms. However, 250 species from 43 genera fulfilled one or more of the criteria to be considered to present a phytosanitary risk. The genera and number of species (in parentheses) considered as posing phytosanitary risk included: Achlysiella (1), Anguina (8), Aphasmatylenchus (1), Aphelenchoides (12), Aphelenchus (1), Belonolaimus (2), Bitylenchus (3), Bursaphelenchus (4), Cactodera (3), Ditylenchus (8), Dolichodorus (1), Globodera (3), Helicotylenchus (7), Hemicriconemoides (3), Hemicycliophora (3), Heterodera (25), Hirschmanniella (5), Hoplolaimus (5), Ibipora (3), Longidorus (10), Macroposthonia (2), Meloidogyne (38), Merlinius (3), Nacobbus (1), Neodolichodorus (2), Paralongidorus (2), Paratrichodorus (11), Paratylenchus (3), Pratylenchus (24), Punctodera (3), Quinisulcius (3), Radopholus (5), Rotylenchulus (3), Rotylenchus (1), Scutellonema (5), Sphaeronema (1), Subanguina (3), Trichodorus (5), Tylenchorhynchus (8), Tylenchulus (2), Vittatidera (1), Xiphinema (15) and Zygotylenchus (1). For each of the 250 species main hosts and yield loss estimates are provided with an extensive bibliography. Of the 250 species, only 126 species from 33 genera are currently listed as regulated pests in one or more countries worldwide. Almost all of these 250 species were also associated with economically important crops and some also acted as vectors for viruses. © 2013 The Authors. Journal compilatio