Low/Hard State Spectra of GRO J1655-40 Observed with Suzaku

The Galactic black-hole binary GRO J1655$-$40 was observed with Suzaku on 2005 September 22--23, for a net exposure of 35 ks with the X-ray Imaging Spectrometer (XIS) and 20 ks with the Hard X-ray Detector (HXD). The source was detected over a broad and continuous energy range of 0.7--300 keV, with an intensity of $\sim$50 mCrab at 20 keV. At a distance of 3.2 kpc, the 0.7--300 keV luminosity is $\sim 5.1 \times 10^{36}$ erg s$^{-1}$ ($\sim 0.7$ % of the Eddington luminosity for a 6 $M_{\odot}$ black hole). The source was in a typical low/hard state, exhibiting a power-law shaped continuum with a photon index of $\sim 1.6$. During the observation, the source intensity gradually decreased by 25% at energies above $\sim 3$ keV, and by 35% below 2 keV. This, together with the soft X-ray spectra taken with the XIS, suggests the presence of an independent soft component that can be represented by emission from a cool ($\sim 0.2$ keV) disk. The hard X-ray spectra obtained with the HXD reveal a high-energy spectral cutoff, with an e-folding energy of $\sim 200$ keV. Since the spectral photon index above 10 keV is harder by $\sim 0.4$ than that observed in the softer energy band, and the e-folding energy is higher than those of typical reflection humps, the entire 0.7--300 keV spectrum cannot be reproduced by a single thermal Comptonization model, even considering reflection effects. Instead, the spectrum (except the soft excess) can be successfully explained by invoking two thermal-Comptonization components with different $y$-parameters. In contrast to the high/soft state spectra of this object in which narrow iron absorption lines are detected with equivalent widths of 60--100 eV, the present XIS spectra bear no such features beyond an upper-limit equivalent width of 25 eV.Comment: 32 pages, 12 figures; accepted for publication in Publications of the Astronomical Society of Japan (PASJ

Urothelium-Specific Deletion of Connexin43 in the Mouse Urinary Bladder Alters Distension-Induced ATP Release and Voiding Behavior

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Intravital imaging of mouse urothelium reveals activation of extracellular signal-regulated kinase by stretch-induced intravesical release of ATP

To better understand the roles played by signaling molecules in the bladder, we established a protocol of intravital imaging of the bladder of mice expressing a Förster/fluorescence resonance energy transfer (FRET) biosensor for extracellular signal-regulated kinase (ERK), which plays critical roles not only in cell growth but also stress responses. With an upright two-photon excitation microscope and a vacuum-stabilized imaging window, cellular ERK activity was visualized in the whole bladder wall, from adventitia to urothelium. We found that bladder distention caused by elevated intravesical pressure (IVP) activated ERK in the urothelium, but not in the detrusor smooth muscle. When bladder distension was prevented, high IVP failed to activate ERK, suggesting that mechanical stretch, but not the high IVP, caused ERK activation. To delineate its molecular mechanism, the stretch-induced ERK activation was reproduced in an hTERT-immortalized human urothelial cell line (TRT-HU1) in vitro. We found that uniaxial stretch raised the ATP concentration in the culture medium and that inhibition of ATP signaling by apyrase or suramin suppressed the stretch-induced ERK activation in TRT-HU1 cells. In agreement with this in vitro observation, pretreatment with apyrase or suramin suppressed the high IVP-induced urothelial ERK activation in vivo. Thus, we propose that mechanical stretch induces intravesical secretion of ATP and thereby activates ERK in the urothelium. Our method of intravital imaging of the bladder of FRET biosensor-expressing mice should open a pathway for the future association of physiological stimuli with the activities of intracellular signaling networks

Intravital imaging of mouse urothelium reveals activation of extracellular signal-regulated kinase by stretch-induced intravesical release of ATP

To better understand the roles played by signaling molecules in the bladder, we established a protocol of intravital imaging of the bladder of mice expressing a Förster/fluorescence resonance energy transfer (FRET) biosensor for extracellular signal-regulated kinase (ERK), which plays critical roles not only in cell growth but also stress responses. With an upright two-photon excitation microscope and a vacuum-stabilized imaging window, cellular ERK activity was visualized in the whole bladder wall, from adventitia to urothelium. We found that bladder distention caused by elevated intravesical pressure (IVP) activated ERK in the urothelium, but not in the detrusor smooth muscle. When bladder distension was prevented, high IVP failed to activate ERK, suggesting that mechanical stretch, but not the high IVP, caused ERK activation. To delineate its molecular mechanism, the stretch-induced ERK activation was reproduced in an hTERT-immortalized human urothelial cell line (TRT-HU1) in vitro. We found that uniaxial stretch raised the ATP concentration in the culture medium and that inhibition of ATP signaling by apyrase or suramin suppressed the stretch-induced ERK activation in TRT-HU1 cells. In agreement with this in vitro observation, pretreatment with apyrase or suramin suppressed the high IVP-induced urothelial ERK activation in vivo. Thus, we propose that mechanical stretch induces intravesical secretion of ATP and thereby activates ERK in the urothelium. Our method of intravital imaging of the bladder of FRET biosensor-expressing mice should open a pathway for the future association of physiological stimuli with the activities of intracellular signaling networks

Whole-Blood Gene Expression Profiles Correlate with Response to Immune Checkpoint Inhibitors in Patients with Metastatic Renal Cell Carcinoma

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Variant allele frequency changes in TP53 predict pembrolizumab response in patients with metastatic urothelial carcinoma

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Laparoscopic excision of an acquired ureteral diverticulum: A case report

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Bmal1 Regulates Prostate Growth via Cell-Cycle Modulation

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Chronoradiation Therapy for Prostate Cancer : Morning Proton Beam Therapy Ameliorates Worsening Lower Urinary Tract Symptoms

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X-Ray and Near-Infrared Observations of GX 339-4 in the Low/Hard State with Suzaku and IRSF

X-ray and near-infrared ($J$-$H$-$K_{\rm s}$) observations of the Galactic black hole binary GX 339--4 in the low/hard state were performed with Suzaku and IRSF in 2009 March. The spectrum in the 0.5--300 keV band is dominated by thermal Comptonization of multicolor disk photons, with a small contribution from a direct disk component, indicating that the inner disk is almost fully covered by hot corona with an electron temperature of $\approx$175 keV. The Comptonizing corona has at least two optical depths, $\tau \approx 1,0.4$. Analysis of the iron-K line profile yields an inner disk radius of $(13.3^{+6.4}_{-6.0}) R_{\rm g}$ ($R_{\rm g}$ represents the gravitational radius $GM/c^2$), with the best-fit inclination angle of $\approx50^\circ$. This radius is consistent with that estimated from the continuum fit by assuming the conservation of photon numbers in Comptonization. Our results suggest that the standard disk of GX 339--4 is likely truncated before reaching the innermost stable circular orbit (for a non rotating black hole) in the low/hard state at $\sim$1% of the Eddington luminosity. The one-day averaged near-infrared light curves are found to be correlated with hard X-ray flux with $F_{\rm Ks} \propto F_{\rm X}^{0.45}$. The flatter near infrared $\nu F_{\nu}$ spectrum than the radio one suggests that the optically thin synchrotron radiation from the compact jets dominates the near-infrared flux. Based on a simple analysis, we estimate the magnetic field and size of the jet base to be $5\times10^4$ G and $6\times 10^8$ cm, respectively. The synchrotron self Compton component is estimated to be approximately 0.4% of the total X-ray flux.Comment: 17pages, 15 figures, accepted for publication in PASJ (Suzaku and MAXI Special Issue