Surface modification of PDMS based microfluidic systems by tensides

The material aspects of a polymer based microfluidic structure were characterised considering the compatibility of the system with bioanalytical applications. The polydimethylsiloxane (PDMS) based channel system is to be integrated in a full polymer photonic biosensor device developed within the European Union project P3SENS (FP7-ICT4-248304). This work is intended to define a modified material composition, which is appropriate to improve both the wettability and the non-specific protein binding characteristics of the PDMS significantly. Triton X-100 (Sigma-Aldrich) surfactant was added to the raw PDMS before polymerisation. The influence of the tenside was studied considering the polymerisation reaction, the surface characteristics and the functional applicability. To test the hydrodynamic behaviour and non-specific protein adsorption on the surfaces, phosphate buffered saline (PBS) solution and fluorescent labelled human serum albumin (HSA) was applied in a microfluidic capillary system. © (2013) Trans Tech Publications, Switzerland

Is The Fe M-shell Absorber Part of The Outflow in Active Galactic Nuclei?

The X-ray emission of many active galactic nuclei (AGNs) is absorbed between 15 and 17 Angstrom by means of unresolved (inner-shell) transition arrays (UTAs) of Fe M-shell ions. The outflow velocities implied by the Doppler shifts of the individual UTAs in the spectrum have never before been measured. Thus, the Fe-M absorber has been commonly assumed to be part of the ionized AGN outflow, whose velocities are readily obtained from more easily measured spectral lines. The best spectrum of Fe-M absorption is available from the integrated 900 ks Chandra HETGS observations of NGC 3783, in which some Fe-M ions are clearly resolved. We measure the velocities of the individual Fe-M ions in NGC 3783 for the first time. Surprisingly, we find that the Fe-M absorber, most noticeably Fe$^{+8}$, Fe$^{+9}$, and Fe$^{+10}$, is not outflowing at the same velocity as the previously known wind. In fact, it appears to be stationary and therefore not part of the outflow at all. It could, alternatively, be ascribed to the skin of the dusty torus. This reduces appreciably the mass loss rate estimated for the NGC 3783 outflow and perhaps for other similar sources as well, in which the various Fe-M ions are not resolved.Comment: To be published in Ap

Density Profiles in Seyfert Outflows

For the past decade, ionized outflows of a few 100 km/s from nearby Seyfert galaxies have been studied in great detail using high resolution X-ray absorption spectra. A recurring feature of these outflows is their broad ionization distribution including essentially ions (e.g., of Fe) from neutral to fully ionized. The absorption measure distribution (AMD) is defined as the distribution of column density with ionization parameter |d N_H/d (log xi)|. AMDs of Seyfert outflows can span up to five orders of magnitude in xi. We present the AMD of five outflows and show that they are all rather flat, perhaps slightly rising towards high ionization. More quantitatively, a power-law fit for log AMD ~ (log xi)^a yields slopes of 0 < a < 0.4. These slopes tightly constrain the density profiles of the wind, which until now could be addressed only by theory. If the wind is distributed on large scales, the measured slopes imply a generic density radial profile of n ~ r^{-alpha} with 1 < alpha < 1.3. This scaling rules out a mass conserving radial flow of n ~ r^{-2}, or a constant density absorber, but is consistent with a non-spherical MHD outflow model in which n ~ r^{-1} along any given line of sight. On the other hand, if ionization variations are a result of local (delta r) density gradients, e.g. as in the turbulent interstellar medium (ISM), the AMD slopes imply density scaling of n ~ delta r^{-alpha} with 0.7 < alpha < 1.0, which is quite different from the scaling of approximately n ~ delta r^{0.4} found in the Milky Way ISM and typical of incompressible turbulence.Comment: submitted to Ap

Construction and Characterization of a Frequency-Controlled, Picometer-Resolution, Displacement Encoder-Actuator

We have constructed an actuator/encoder whose generated displacement is controlled through the resonance frequency of a microwave cavity. A compact, 10-micrometer-range, digitally-controlled actuator executing frequency-coded displacement with picometer resolution is described. We consider this approach particularly suitable for metrologic-precision scanning probe microscopy.Comment: 4 pages, 4 figure

Demonstration of NV-detected ESR spectroscopy at 115 GHz and 4.2 Tesla

High frequency electron spin resonance (ESR) spectroscopy is an invaluable tool for identification and characterization of spin systems. Nanoscale ESR using the nitrogen-vacancy (NV) center has been demonstrated down to the level of a single spin. However, NV-detected ESR has exclusively been studied at low magnetic fields, where spectral overlap prevents clear identification of spectral features. Within this work, we demonstrate NV-detected ESR measurements of single-substitutional nitrogen impurities in diamond at a NV Larmor frequency of 115 GHz and the corresponding magnetic field of 4.2 Tesla. The NV-ESR measurements utilize a double electron-electron resonance sequence and are performed using both ensemble and single NV spin systems. In the single NV experiment, chirp pulses are used to improve the population transfer and for NV-ESR measurements. This work provides the basis for NV-based ESR measurements of external spins at high magnetic fields.Comment: 12 pages, 4 figure

Making Rents More Affordable: The Rental Housing Support Program

The Chicago Coalition for the Homeless (CCH) and the Statewide Housing Action Coalition (SHAC), through the "It Takes a Home to Raise a Child" campaign, have proposed state legislation that creates a revenue stream to fund local rental subsidy programs throughout the state. Under this legislation, known as the Rental Housing Support Program, the Illinois Housing Development Authority (IHDA) would offer grants either to local administering agencies (local governmental bodies, local housing authorities, not-for-profit organizations) or to developers of affordable housing

Time variation of Kepler transits induced by stellar rotating spots - a way to distinguish between prograde and retrograde motion I. Theory

Some transiting planets discovered by the Kepler mission display transit timing variations (TTVs) induced by stellar spots that rotate on the visible hemisphere of their parent stars. An induced TTV can be observed when a planet crosses a spot and modifies the shape of the transit light curve, even if the time resolution of the data does not allow to detect the crossing event itself. We present an approach that can, in some cases, use the derived TTVs of a planet to distinguish between a prograde and a retrograde planetary motion with respect to the stellar rotation. Assuming a single spot darker than the stellar disc, spot crossing by the planet can induce measured positive (negative) TTV, if the crossing occurs in the first (second) half of the transit. On the other hand, the motion of the spot towards (away from) the center of the stellar visible disc causes the stellar brightness to decrease (increase). Therefore, for a planet with prograde motion, the induced TTV is positive when the local slope of the stellar flux at the time of transit is negative, and vice versa. Thus, we can expect to observe a negative (positive) correlation between the TTVs and the photometric slopes for prograde (retrograde) motion. Using a simplistic analytical approximation, and also the publicly available SOAP-T tool to produce light curves of transits with spot-crossing events, we show for some cases how the induced TTVs depend on the local stellar photometric slopes at the transit timings. Detecting this correlation in Kepler transiting systems with high enough signal-to-noise ratio can allow us to distinguish between prograde and retrograde planetary motions. In coming papers we present analyses of the KOIs and Kepler eclipsing binaries, following the formalism developed here.Comment: V2: Major revision, accepted to Ap

X-Ray Absorption Analysis of MCG-6-30-15: Discerning Three Kinematic Systems

By analyzing the X-ray spectrum of MCG-6-30-15 obtained with the HETG spectrometer on board the Chandra Observatory, we identify three kinematically distinct absorption systems; two outflow components intrinsic to MCG-6-30-15, and one local at z = 0. The slow outflow at -100 +/- 50 km s^-1 has a large range of ionization manifested by absorption from 24 different charge states of Fe, which enables a detailed reconstruction of the absorption measure distribution (AMD). This AMD spans five orders of magnitude in ionization parameter: -1.5 < log xi < 3.5 (cgs units), with a total column density of N_H = (5.3 +/- 0.7) x 10^21 cm^-2. The fast outflow at -1900 +/- 150 km s^-1 has a well defined ionization parameter with log xi = 3.82 +/- 0.03 (cgs units) and column density N_H = 8.1 +/- 0.7 x 10^22 cm^-2. Assuming this component is a thin, uniform, spherical shell, it can be estimated to lie within 11 light days of the \agn center. The third component, most clearly detected in the lower oxygen charge states O^+1 - O^+6, has been confused in the past with the fast outflow, but is identified here with local gas z = 0 and a total column density N_H of a few 10^20 cm^-2. Finally, we exploit the excellent spectral resolution of the HETGS and use the present spectrum to determine the rest-frame wavelengths of oxygen inner-shell lines that were previously uncertain.Comment: 34 pages, 6 figures, submitted to Ap

Machine tools safety

V této bakalářské práci je vypracován přehled legislativních požadavků a směrnic požadovaných dle Evropského parlamentu a Rady v oblasti obráběcích strojů. Dále se práce zabývá harmonizovanými normami v této oblasti. Bezpečnostní požadavky jsou následně ukázány na vybraném obráběcím stroji - radiální vrtačce.This thesis contains an overview of safety requirements and standarts by European Parliament and Council in field of machine tools. The other chapter is about harmonized standards of machine tools. Safety requirements are shown on chosen machine tool - radial drill.