Estimation of carbon pools in the biomass and soil of mangrove forests in Sirik Azini creek, Hormozgan province (Iran)

Despite the increasing interest in mangroves as one of the most carbon-rich ecosystems, arid mangroves are still poorly investigated. We aimed to improve the knowledge of biomass and soil carbon sequestration for an arid mangrove forest located at the Azini creek, Sirik, Hormozgan Province (Iran). We investigated the biomass and organic carbon stored in the above and belowground biomass for three different regions selected based on the composition of the principal species: (1) Avicennia marina, (2) mixed forest of A. marina and Rhizophora mucronata, and (3) R. mucronata. Topsoil organic carbon storage to 30 cm depth was also estimated for each analyzed area. Biomass carbon storage, considering both aboveground (AGB) and belowground biomass (BGB), was significantly different between the cover areas. Overall, the mean forest biomass (MFB) was 283.1 ± 89 Mg C ha−1 with a mean C stored in the biomass of 128.9 ± 59 Mg C ha−1. Although pure Rhizophora stand showed the lowest value of above and below tree carbon (AGC + BGC); 17.6 ± 1.9 Mg C ha−1), soil organic carbon stock in sites under Rhizophora spp. was significantly higher than in the site with pure stand of Avicennia spp. Overall, forest soil stored the highest proportion of Sirik mangrove ecosystem organic carbon (59%), with a mean value of 188.3 ± 27 Mg C ha−1. These results will contribute to broaden the knowledge and the dataset available, reducing the uncertainties related to estimates and modeling of carbon pools in arid mangrove ecosystem, which also represent an important climatic threshold of mangrove worldwide distribution

Novel additive for sperm cryopreservation media: Holotheria parva coelomic cavity extract protects human spermatozoa against oxidative stress—A pilot study

Cryopreservation is the most effective method for preserving semen for a long period of time. However, during the freeze–thaw process, production of reactive oxygen species (ROS) leads to a steep reduction in sperm fertility indices. In this study, we tested the effects of the extract of the coelomic cavity of five Holotheria parva, a marine organism rich in antioxidants, for its ROS-scavenging activity and cryoprotective effects on oxidative stress. Using a total of 50 semen samples, our results demonstrated that doses of 250 and 500 µg/ml of H. parva coelomic cavity extract significantly increased sperm vitality as compared to the control (p <.05). The addition of 250 µg/ml of the extract exerted a significant positive effect on sperm motility. Moreover, sperm DNA damage and ROS production were significantly reduced at extract concentrations of 250 and 500 µg/ml (p <.05). To the best of our knowledge, the results of this study represent the first demonstration of the possibility of improving sperm parameters and reducing ROS production and DNA damage by supplementing sperm freezing media with H. parva coelomic extract. Our results suggested that H. parva coelomic extract could be useful for improving the fertilising ability of frozen-thawed human semen

Path analysis of the relationship between optimism, humor, affectivity, and marital satisfaction among infertile couples

Marital satisfaction is an important factor for establishing a family relationship, feeling satisfied, and living happily together. The aim of the present study was to investigate the relationship between optimism, humor, positive and negative affect, and marital satisfaction among infertile couples. The sample comprised 80 infertile Iranian couples (n = 160) who visited infertility clinics. Participants completed a series of Persian versions of psychometric scales related to optimism (Attributional Style Questionnaire), humor (Humor Styles Questionnaire), marital satisfaction (Enrich Marital Satisfaction Questionnaire), positive affect (PA) and negative affect (NA) (Positive and Negative Affect Schedule). The obtained result of Smart PLS statistical analysis confirmed the significant positive correlation between optimism and humor with marital satisfaction and high PA and low NA. Moreover, the findings also provided an adequate fit of the model. The findings demonstrated that infertile couples high in optimism and humor have higher levels of marital satisfaction and high PA and low PA. Based on the study’s findings, interventions for facilitating optimism and humor among infertile couples are discussed

Purification and immobilization of engineered glucose dehydrogenase: A new approach to producing gluconic acid from breadwaste

Background Platform chemicals are essential to industrial processes. Used as starting materials for the manufacture of diverse products, their cheap availability and efficient sourcing are an industrial requirement. Increasing concerns about the depletion of natural resources and growing environmental consciousness have led to a focus on the economics and ecological viability of bio-based platform chemical production. Contemporary approaches include the use of immobilized enzymes that can be harnessed to produce high-value chemicals from waste. Results In this study, an engineered glucose dehydrogenase (GDH) was optimized for gluconic acid (GA) production. Sulfolobus solfataricus GDH was expressed in Escherichia coli. The Km and Vmax values for recombinant GDH were calculated as 0.87 mM and 5.91 U/mg, respectively. Recombinant GDH was immobilized on a hierarchically porous silica support (MM-SBA-15) and its activity was compared with GDH immobilized on three commercially available supports. MM-SBA-15 showed significantly higher immobilization efficiency (> 98%) than the commercial supports. After 5 cycles, GDH activity was at least 14% greater than the remaining activity on commercial supports. Glucose in bread waste hydrolysate was converted to GA by free-state and immobilized GDH. After the 10th reuse cycle on MM-SBA-15, a 22% conversion yield was observed, generating 25 gGA/gGDH. The highest GA production efficiency was 47 gGA/gGDH using free-state GDH. Conclusions This study demonstrates the feasibility of enzymatically converting BWH to GA: immobilizing GDH on MM-SBA-15 renders the enzyme more stable and permits its multiple reuse

Fatigue resistance of CAD/CAM crowns restored on premolars

The Congress was held in conjunction with 68th Meeing of the Japanese Society for Dental Materials and Devices, and 2016 Annual Meeting of the Indonesian Society for Dental Materials Science and TechnologyOral Presentation V: Clinical Application - paper no. O-2