Recombinant Lactococcus lactis NZ9000 secretes a bioactive kisspeptin that inhibits proliferation and migration of human colon carcinoma HT-29 cells

BACKGROUND: Proteinaceous bioactive substances and pharmaceuticals are most conveniently administered orally. However, the facing problems are the side effects of proteolytic degradation and denaturation in the gastrointestinal tract. In recent years, lactic acid bacteria (LAB) have been verified to be a promising delivery vector for susceptible functional proteins and drugs. KiSS-1 peptide, a cancer suppressor, plays a critical role in inhibiting cancer metastasis and its activity has been confirmed by direct administration. However, whether this peptide can be functionally expressed in LAB and exert activity on cancer cells, thus providing a potential alternative administration manner in the future, has not been demonstrated. RESULTS: A recombinant Lactococcus lactis strain NZ9000-401-kiss1 harboring a plasmid containing the gene of the tumor metastasis-inhibiting peptide KiSS1 was constructed. After optimization of the nisin induction conditions, the recombinant strain efficiently secreted KiSS1 with a maximum detectable amount of 27.9 μg/ml in Dulbecco’s Modified Eagle medium. The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide and would healing assays, respectively, indicated that the secreted KiSS1 peptide remarkably inhibited HT-29 cell proliferation and migration. Furthermore, the expressed KiSS1 was shown to induce HT-29 cell morphological changes, apoptosis and reduce the expression of matrix metalloproteinase 9 (MMP-9) at both mRNA and protein levels. CONCLUSIONS: A recombinant L. lactis NZ9000-401-kiss1 successfully expressing the human kiss1 was constructed. The secreted KiSS1 peptide inhibited human HT-29 cells’ proliferation and migration probably by invoking, or mediating the cell-apoptosis pathway and by down regulating MMP-9 expression, respectively. Our results suggest that L. lactis is an ideal cell factory for secretory expression of tumor metastasis-inhibiting peptide KiSS1, and the KiSS1-producing L. lactis strain may serve as a new tool for cancer therapy in the future

Variation detection based on next-generation sequencing of type Chinese 1 strains of Toxoplasma gondii with different virulence from China

A: Summary of annotation for SNPs; B: Summary of annotation for indels; C: Summary of annotation for SVs; D: Summary of annotation for CNVs. (DOCX 18 kb

Quantum Truncated Differential and Boomerang Attack

Facing the worldwide steady progress in building quantum computers, it is crucial for cryptographic community to design quantum-safe cryptographic primitives. To achieve this, we need to investigate the capability of cryptographic analysis tools when used by the adversaries with quantum computers. In this article, we concentrate on truncated differential and boomerang cryptanalysis. We first present a quantum algorithm which is designed for finding truncated differentials of symmetric ciphers. We prove that, with a overwhelming probability, the truncated differentials output by our algorithm must have high differential probability for the vast majority of keys in key space. Afterwards, based on this algorithm, we design a quantum algorithm which can be used to find boomerang distinguishers. The quantum circuits of both quantum algorithms contain only polynomial quantum gates. Compared to classical tools for searching truncated differentials or boomerang distinguishers, our algorithms fully utilize the strengths of quantum computing, and can maintain the polynomial complexity while fully considering the impact of S-boxes and key scheduling