Perbedaan Kualitas Spermatozoa Mencit Jantan (Mus Musculus L) Yang Diberikan Vitamin C Setelah Pemaparan Asap Rokok

: Vitamin C is an antioxidant that suppresses the oxidative stress caused by cigarette smoke purpose of this study demonstrate whether vitamin C can provide a different quality of sperm male mice Mus musculus L are given exposure to cigarette smoke. Methodology: The study subjects are 32 male mice Mus musculus L, which were randomly divided into 2 groups. The first group was the control group (K) who received treatment exposure from cigarette smoke without giving vitamin C and the second group is the treatment group (P) are given exposure from cigarette smoke and vitamin C dose of 0.40 mg / gBB /day. The treatment during 30 days, on day 31 the mice were terminated, and then examined the quality of spermatozoa motility of spermatozoa, sperm concentration and morphology of spermatozoa. Results: Test oneway ANOVA on the mean motility of spermatozoa showed Ma control group (47,38%), Mb (11,88%), Mc (18,13%), Md (22,63%) and the Ma treatment group (11.68%), Mb (17,04%), Mc (35,77%), Md (16,5%). The mean concentration of spermatozoa obtained 63,34x105/ml control group and the treatment group and the mean morphology of spermatozoa 81,75x105/ml control group showed normal morphology 50% and abnormal morphology 56,189% while the treatment group showed normal morphology 60% and 40% abnormal. Result from Oneway ANOVA test there are differences significant in the morphology of spermatozoa, concentration of spermatozoa, sperm concentration, and sperm morphology between groups (p <0,05). Conclusion: Vitamin C can improve the quality of spermatozoa after exposure to cigarette smoke

Application of Strengthening Detail Management in Intensive-Care Unit Nursing Management

As the core work of the hospital, the intensive care unit (ICU) has gathered many of the most cutting-edge facilities and related medical staff. ICU is the core application way to treat high-risk patients, which often contains the deep hope of patients and their families. Therefore, it is critical to further improve the quality of care management and control in the intensive-care unit. The use of in-depth detail controls is critical to the daily care of patients in intensive-care unit, so it's worth exploring further.Â

A Comparative Study of Patients’ Attitudes Toward Clinical Research in the United States and Urban and Rural China

As the number of clinical trials conducted in China increases, understanding Chinese attitudes toward clinical research is critical for designing effective and ethical studies. Two survey studies were conducted in 2012 and 2013 to compare patient attitudes toward clinical research and factors affecting research participation in the United States and urban and rural China. We surveyed 525 patients in 2012 (186 US, 186 urban, 153 rural China) and 690 patients in 2013 (412 US, 206 urban, 72 rural China). US patients were more likely to have no concerns regarding research participation than Chinese patients. Most common concerns of US patients were safety, privacy and confidentiality, and time required. Safety was a top concern for many Chinese. Chinese patients, particularly rural Chinese, were more concerned about the likelihood of self‐benefit, and receiving free medical care and financial incentive had greater influence on their participation. Being informed of the freedom to choose whether to participate or to leave a study was less important to Chinese patients. Our study provides important insights into Chinese patients' attitudes toward clinical research and the need to educate them about their rights. These findings help in designing cross‐cultural clinical studies that maximize enrollment while upholding Western ethical standards.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/111170/1/cts12254.pd

Game Theory Model of Traffic Participants within Amber Time at Signalized Intersection

The traffic light scheme is composed of red, green, and amber lights, and it has been defined clearly for the traffic access of red and green lights; however, the definition of that for the amber light is indistinct, which leads to the appearance of uncertainty factors and serious traffic conflicts during the amber light. At present, the traffic administrations are faced with the decision of whether to forbid passing or not during the amber light in the cities of China. On one hand, it will go against the purpose of setting amber lights if forbidding passing; on the other hand, it may lead to a mess of traffic flow running if not. And meanwhile the drivers are faced with the decision of passing the intersection or stopping during the amber light as well. So the decision-making behavior of traffic administrations and drivers can be converted into a double game model. And through quantification of their earnings in different choice conditions, the optimum decision-making plan under specific conditions could be solved via the Nash equilibrium solution concept. Thus the results will provide a basis for the formulation of the traffic management strategy.</jats:p

RNA-seq for gene identification and transcript profiling in relation to root growth of bermudagrass (Cynodon dactylon) under salinity stress

BACKGROUND: Soil salinity is one of the most significant abiotic stresses affecting plant shoots and roots growth. The adjustment of root architecture to spatio-temporal heterogeneity in salinity is particularly critical for plant growth and survival. Bermudagrass (Cynodon dactylon) is a widely used turf and forage perennial grass with a high degree of salinity tolerance. Salinity appears to stimulate the growth of roots and decrease their mortality in tolerant bermudagrass. To estimate a broad spectrum of genes related to root elongation affected by salt stress and the molecular mechanisms that control the positive response of root architecture to salinity, we analyzed the transcriptome of bermudagrass root tips in response to salinity. RESULTS: RNA-sequencing was performed in root tips of two bermudagrass genotypes contrasting in salt tolerance. A total of 237,850,130 high quality clean reads were generated and 250,359 transcripts were assembled with an average length of 1115 bp. Totally, 103,324 unigenes obtained with 53,765 unigenes (52 %) successfully annotated in databases. Bioinformatics analysis indicated that major transcription factor (TF) families linked to stress responses and growth regulation (MYB, bHLH, WRKY) were differentially expressed in root tips of bermudagrass under salinity. In addition, genes related to cell wall loosening and stiffening (xyloglucan endotransglucosylase/hydrolases, peroxidases) were identified. CONCLUSIONS: RNA-seq analysis identified candidate genes encoding TFs involved in the regulation of lignin synthesis, reactive oxygen species (ROS) homeostasis controlled by peroxidases, and the regulation of phytohormone signaling that promote cell wall loosening and therefore root growth under salinity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-1799-3) contains supplementary material, which is available to authorized users

Plasma D-dimer and prothrombin fragment 1+2 in evaluating the occurrence of venous thromboembolism in advanced cancer patients and the effect of preventive anticoagulant therapy

Background: This study aimed to evaluate the role of D-dimer (DD) and prothrombin fragment 1+2 (PF1+2) in assessing the risk of venous thromboembolism (VTE) in advanced cancer patients, as well as the impact of preventive anticoagulant therapy. Methods: A total of 137 advanced cancer patients admitted to the 908th Hospital of the Chinese People's Liberation Army Joint Logistic Support Force Hospital between February 2023 and June 2024 were included. Patients were divided into two groups based on the presence (VTE group, n=49) or absence (non-VTE group, n=88) of VTE. Blood tests were performed at admission, and the relationship between DD, PF1+2, and VTE risk was analysed. Patients without VTE were further categorised into two treatment groups: the preventive anticoagulant treatment group (AG, n=48) and the conventional treatment group (RT, n=40), based on their preference. The incidence of VTE in both groups was compared to assess the effectiveness of preventive anticoagulant therapy. Results: Before chemotherapy, DD levels were significantly higher in patients who developed VTE than those who did not. Both DD and PF1+2 were found to be independent risk factors for VTE after chemotherapy. The incidence of VTE was lower in the AG group than in the RT group, with a statistically significant difference. Conclusions: DD and PF1+2 are reliable indicators for assessing VTE risk in advanced cancer patients and can help guide the use of preventive anticoagulant therapy

Phenotypic Pattern-Based Assay for Dynamically Monitoring Host Cellular Responses to Salmonella Infections

The interaction between mammalian host cells and bacteria is a dynamic process, and the underlying pathologic mechanisms are poorly characterized. Limited information describing the host-bacterial interaction is based mainly on studies using label-based endpoint assays that detect changes in cell behavior at a given time point, yielding incomplete information. In this paper, a novel, label-free, real-time cell-detection system based on electronic impedance sensor technology was adapted to dynamically monitor the entire process of intestinal epithelial cells response to Salmonella infection. Changes in cell morphology and attachment were quantitatively and continuously recorded following infection. The resulting impedance-based time-dependent cell response profiles (TCRPs) were compared to standard assays and showed good correlation and sensitivity. Biochemical assays further suggested that TCRPs were correlated with cytoskeleton-associated morphological dynamics, which can be largely attenuated by inhibitions of actin and microtubule polymerization. Collectively, our data indicate that cell-electrode impedance measurements not only provide a novel, real-time, label-free method for investigating bacterial infection but also help advance our understanding of host responses in a more physiological and continuous manner that is beyond the scope of current endpoint assays

Simulation of medium- and long-term crude oil demand scenarios in China based on system dynamics

Objective With the implementation of the "dual carbon" strategy and the rapid development of the new energy system, there is consensus in the industry that China's crude oil consumption is approaching a plateau and will gradually decline. In this context, analyzing the medium- and long-term trends in crude oil demand and identifying the key driving factors are crucial for the steady development of the national crude oil industry, the formulation and evaluation of energy policies, and the advancement of the new energy system. Methods Using the system dynamics method, a scenario analysis model for China's medium- and long-term crude oil demand was developed, comprising five subsystems: economy, population, crude oil supply, science and technology, and environment. Considering the overall development status and future feasibility of these subsystems, six distinct scenarios were created to simulate China's crude oil consumption demand from 2023 to 2070, including a baseline scenario, a high economic growth scenario, and an energy substitution scenario. Results Under different development scenarios, the peaking time, peak value and development path of crude oil demand varied slightly, but the overall trends remained consistent. In most scenarios, crude oil demand would peak between 2028 and 2031, entering a plateau period (7.5×108–7.8×108 t) before beginning a gradual decline. By around 2050, the rate of decline would slow down, leading to a new plateau around 2060, with demand estimated at approximately 2×108 t. Prior to 2050, demand for crude oil as fuel would be greater than that for crude oil as feedstock, primarily for use in the transportation sector. After 2050, the demand for crude oil as feedstock would surpass that for crude oil as fuel, establishing it as the dominant consumption attribute. Economic development and investment in science and technology were identified as the primary drivers influencing crude oil demand. Conclusion To ensure the steady development of China's crude oil industry, the following recommendations are proposed: continually promote the transformation and upgrading of the industrial structure, improve crude oil utilization efficiency, accelerate the development of low- and zero-carbon substitutes, encourage clean consumption of crude oil, increase investment in exploration and development, and enhance scientific and technological innovation to support research and development. These measures will help ensure the coordinated and stable advancement of China's social economy, energy transition, and energy supply security