1,549 research outputs found

    Monoclonal antibodies against human astrocytomas and their reactivity pattern

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    The establishment of hybridomas after fusion of X63-Ag8.653 mouse myeloma cells and splenocytes from mice hyperimmunized against human astrocytomas is presented. The animals were primed with 5 × 106 chemically modified uncultured or cultured glioma cells. Six weeks after the last immunization step an intrasplenal booster injection was administrated and 3 days later the spleen cells were prepared for fusion experiments. According to the specificity analysis of the generated antibodies 7 hybridoma products (MUC 7-22, MUC 8-22, MUC 10-22, MUC 11-22, MUC 14-22, MUC 15-22 and MUC 2-63) react with gliomas, neuroblastomas and melanomas as well as with embryonic and fetal cells but do not recognize non-neurogenic tumors. The selected monoclonal antibodies (McAbs) of IgG1 and IgG2a isotypes are not extensively characterized but these antibodies have been demonstrated to be reactive with a panel of glioma cell lines with varying patterns of antigen distribution. Using the McAbs described above and a series of cryosections of glioma biopsies and paraffin sections of the same material as well as glioma cultures established from these, variable antigenic profiles among glioma cell populations could be demonstrated. From these results it is evident that there is not only a distinct degree of antigenic heterogeneity among and within brain tumors, but also that the pattern of antigenic expression can change continuously. Some of the glioma associated antigens recognized by the selected antibodies persist after fixation with methanol/acetone and Karnovsky's fixative and probably are oncoembryonic/oncofetal antigen(s). The data suggest that the use of McAbs recognizing tumor associated oncofetal antigens in immunohistochemistry facilitates objective typing of intracranial malignancies and precise analysis of fine needle brain/tumor biopsies in a sensitive and reproducible manner

    The SPARC Data Initiative: comparisons of CFC-11, CFC-12, HF and SF6 climatologies from international satellite limb sounders

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    A quality assessment of the CFC-11 (CCl3F), CFC-12 (CCl2F2), HF, and SF6 products from limb-viewing satellite instruments is provided by means of a detailed intercomparison. The climatologies in the form of monthly zonal mean time series are obtained from HALOE, MIPAS, ACE-FTS, and HIRDLS within the time period 1991–2010. The intercomparisons focus on the mean biases of the monthly and annual zonal mean fields and aim to identify their vertical, latitudinal and temporal structure. The CFC evaluations (based on MIPAS, ACE-FTS and HIRDLS) reveal that the uncertainty in our knowledge of the atmospheric CFC-11 and CFC-12 mean state, as given by satellite data sets, is smallest in the tropics and mid-latitudes at altitudes below 50 and 20 hPa, respectively, with a 1σ multi-instrument spread of up to ±5 %. For HF, the situation is reversed. The two available data sets (HALOE and ACE-FTS) agree well above 100 hPa, with a spread in this region of ±5 to ±10 %, while at altitudes below 100 hPa the HF annual mean state is less well known, with a spread ±30 % and larger. The atmospheric SF6 annual mean states derived from two satellite data sets (MIPAS and ACE-FTS) show only very small differences with a spread of less than ±5 % and often below ±2.5 %. While the overall agreement among the climatological data sets is very good for large parts of the upper troposphere and lower stratosphere (CFCs, SF6) or middle stratosphere (HF), individual discrepancies have been identified. Pronounced deviations between the instrument climatologies exist for particular atmospheric regions which differ from gas to gas. Notable features are differently shaped isopleths in the subtropics, deviations in the vertical gradients in the lower stratosphere and in the meridional gradients in the upper troposphere, and inconsistencies in the seasonal cycle. Additionally, long-term drifts between the instruments have been identified for the CFC-11 and CFC-12 time series. The evaluations as a whole provide guidance on what data sets are the most reliable for applications such as studies of atmospheric transport and variability, model–measurement comparisons and detection of long-term trends. The data sets will be publicly available from the SPARC Data Centre and through PANGAEA (doi:10.1594/PANGAEA.849223)

    Variational Calculation of the Effective Action

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    An indication of spontaneous symmetry breaking is found in the two-dimensional λϕ4\lambda\phi^4 model, where attention is paid to the functional form of an effective action. An effective energy, which is an effective action for a static field, is obtained as a functional of the classical field from the ground state of the hamiltonian H[J]H[J] interacting with a constant external field. The energy and wavefunction of the ground state are calculated in terms of DLCQ (Discretized Light-Cone Quantization) under antiperiodic boundary conditions. A field configuration that is physically meaningful is found as a solution of the quantum mechanical Euler-Lagrange equation in the J0J\to 0 limit. It is shown that there exists a nonzero field configuration in the broken phase of Z2Z_2 symmetry because of a boundary effect.Comment: 26 pages, REVTeX, 7 postscript figures, typos corrected and two references adde

    Modification of the ω\omega-Meson Lifetime in Nuclear Matter

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    The photo production of ω\omega mesons on the nuclei C, Ca, Nb and Pb has been measured using the Crystal Barrel/TAPS detector at the ELSA tagged photon facility in Bonn. The dependence of the ω\omega meson cross section on the nuclear mass number has been compared with three different types of models, a Glauber analysis, a BUU analysis of the Giessen theory group and a calculation by the Valencia theory group. In all three cases, the inelastic ω\omega width is found to be 130150MeV/c2130-150 \rm{MeV/c^2} at normal nuclear matter density for an average 3-momentum of 1.1 GeV/c. In the restframe of the ω\omega meson, this inelastic ω\omega width corresponds to a reduction of the ω\omega lifetime by a factor 30\approx 30. For the first time, the momentum dependent ω\omegaN cross section has been extracted from the experiment and is in the range of 70 mb.Comment: 5 pages, 4 figure

    High statistics study of the reaction γpp  2π0\gamma p\to p\;2\pi^0

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    The photoproduction of 2π0\pi^0 mesons off protons was studied with the Crystal Barrel/TAPS experiment at the electron accelerator ELSA in Bonn. The energy of photons produced in a radiator was tagged in the energy range from 600\,MeV to 2.5\,GeV. Differential and total cross sections and pπ0π0p\pi^0\pi^0 Dalitz plots are presented. Part of the data was taken with a diamond radiator producing linearly polarized photons, and beam asymmetries were derived. Properties of nucleon and Δ\Delta resonances contributing to the pπ0π0p\pi^0\pi^0 final state were determined within the BnGa partial wave analysis. The data presented here allow us to determine branching ratios of nucleon and Δ\Delta resonances for their decays into pπ0π0p\pi^0\pi^0 via several intermediate states. Most prominent are decays proceeding via Δ(1232)π\Delta(1232)\pi, N(1440)1/2+πN(1440)1/2^+\pi, N(1520)3/2πN(1520)3/2^-\pi, N(1680)5/2+πN(1680)5/2^+\pi, but also pf0(500)pf_0(500), pf0(980)pf_0(980), and pf2(1270)pf_2(1270) contribute to the reaction.Comment: 28 pages, 17 figures, 7 table

    Photoproduction of pi0 omega off protons for E(gamma) < 3 GeV

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    Differential and total cross-sections for photoproduction of gamma proton to proton pi0 omega and gamma proton to Delta+ omega were determined from measurements of the CB-ELSA experiment, performed at the electron accelerator ELSA in Bonn. The measurements covered the photon energy range from the production threshold up to 3GeV.Comment: 8 pages, 13 figure

    In-medium ω\omega mass from the γ+Nbπ0γ+X\gamma + Nb \to \pi^{0}\gamma + X reaction

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    Data on the photoproduction of ω\omega mesons on nuclei have been re-analyzed in a search for in-medium modifications. The data were taken with the Crystal Barrel(CB)/TAPS detector system at the ELSA accelerator facility in Bonn. First results from the analysis of the data set were published by D. Trnka et al. in Phys. Rev. Lett 94 (2005) 192303 \cite{david}, claiming a lowering of the ω\omega mass in the nuclear medium by 14% at normal nuclear matter density. The extracted ω\omega line shape was found to be sensitive to the background subtraction. For this reason a re-analysis of the same data set has been initiated and a new method has been developed to reduce the background and to determine the shape and absolute magnitude of the background directly from the data. Details of the re-analysis and of the background determination are described. The ω\omega signal on the NbNb target, extracted in the re-analysis, does not show a deviation from the corresponding line shape on a LH2LH_2 target, measured as reference. The earlier claim of an in-medium mass shift is thus not confirmed. The sensitivity of the ω\omega line shape to different in-medium modification scenarios is discussed.Comment: 13 pages and 11 figures, submitted for publicatio

    Quasi-free photoproduction of eta-mesons of the neutron

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    Quasi-free photoproduction of eta-mesons off nucleons bound in the deuteron has been measured with the CBELSA/TAPS detector for incident photon energies up to 2.5 GeV at the Bonn ELSA accelerator. The eta-mesons have been detected in coincidence with recoil protons and recoil neutrons, which allows a detailed comparison of the quasi-free n(gamma,eta)n and p(gamma,eta)p reactions. The excitation function for eta-production off the neutron shows a pronounced bump-like structure at W=1.68 GeV (E_g ~ 1 GeV), which is absent for the proton.Comment: accepted for publication in Phys. Rev. Let
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