Insertion of short hepatitis virus A amino acid sequences into poliovirus antigenic determinants results in viable progeny

AbstractIn an infectious poliovirus cDNA construct, the determinant encoding antigenic epitope N-Agl (in a loop located between two β-strands in polypeptide VP1) was altered by site-directed mutagenesis, to be partially similar with the determinants for presumptive epitopes in polypeptides VP1 or VP3 of hepatitis A virus (HAV). The modified constructs proved to be infectious. However, another construct, in which the same locus encoded a ‘nonsense’ and a relatively hydrophobic amino acid sequence, exhibited no infectivity. These data showed the feasibility of the insertion of foreign sequences in a specific antigenically active locus of the poliovirus icosahedron, and suggest some limitations with respect to the sequences to be ‘transplanted’

Reliability Testing, Noise And Error Correction Of Real Quantum Computing Devices

From Pharmacology to Cryptography and from Geology to Astronomy are some of the scientific fields in which Quantum Computing potentially will take off and fly high. Big Quantum Computing vendors invest a large amount of money in improving the hardware and they claim that soon enough a quantum program will be hundreds of thousands of times faster than a typical one we know nowadays. But still the reliability of such systems is the main obstacle. In this work, the reliability of real quantum devices is tested and techniques of noise and error correction are presented while measurement error mitigation is explored. In addition, a wellknown string matching algorithm (Bernstein–Vazirani) was applied to the real quantum computing device in order to measure its accuracy and reliability. Simulated environments were also used in order to evaluate the results. The results obtained, even if these were not 100% accurate, are very promising which proves that even these days a quantum computer working side by side with a typical one is reliable and especially when error mitigation techniques are applied. © 2021,Telfor Journal. All Rights Reserved

Effective method for obtaining long nucleotide chains on partially complementary templates Processed bovine γ-interferon gene obtained from human γ-interferon gene

AbstractThe method of obtaining the bovine γ-interferon gene by means of simultaneous multidirected mutagenesis of the human γ-interferon gene is presented. The first strand of the bovine γ-interferon gene was obtained by ligation of synthetic oligonucleotides, using the cDNA of human γ-interferon, cloned in the single-stranded phage M13mpl9 as a template. The second strand was synthesized using a large fragment of E. coli DNA-polymerase 1. The double-stranded gene was then treated by restriction nucleases and cloned in a pUC- 18 derived vector. The primary structure was confirmed by sequencing