Isolation of respiratory syncytial virus from nasopharyngeal aspirates stored at 20ºC from one to fifteen months after collection

Cell culture isolation is used for recovering respiratory syncytial virus (RSV) from respiratory specimens. As RSV is a thermolabile virus, specimens destined for inoculation into cell culture require special transport, handling, and storage. The isolation rate of RSV from nasopharyngeal aspirates (NPA) stored at 20ºC for one to 15 months after collection was investigated. A total of 126 samples considered positive for RSV by indirect fluorescence-antibody were tested by virus isolation in HEp-2 cell culture. RSV was isolated from 47/126 specimens (37.3%). These results show that RSV may be recovered from NPA stored at 20ºC by cell culture

Restaurantes self-service: segurança e qualidade sanitária dos alimentos servidos Self-service restaurants: food safety and sanitary quality

OBJETIVO: Esse trabalho teve por objetivo avaliar a temperatura e a qualidade sanitária de alimentos servidos nos balcões de distribuição em restaurantes self-service no município de Taubaté (SP), com vistas à discussão da segurança alimentar. MÉTODOS: Foram coletadas quatro amostras, sendo dois alimentos quentes e dois alimentos frios de 16 restaurantes; antes da coleta mediu-se a temperatura dos alimentos expostos nos balcões de distribuição. As amostras foram analisadas quanto aos seguintes parâmetros: contagem de bactérias aeróbias mesófilas, determinação de coliformes a 35ºC e a 45ºC, contagem de Staphylococcus coagulase positiva e detecção de Salmonella. RESULTADOS: Cinquenta e seis (87,5%) alimentos estavam em temperaturas inadequadas, alimentos frios expostos em temperaturas acima de 10ºC e alimentos quentes abaixo de 60ºC. Bactérias aeróbias mesófilas em contagem acima de 10(6) UFC/g foram detectadas em 11% das amostras e coliformes a 35ºC e coliformes a 45ºC foram detectadas em 76,6% e 43,7% das amostras, respectivamente. Dois alimentos, sendo um frio e um quente, provenientes de um mesmo restaurante, apresentaram contagem de Staphylococcus coagulase positivo acima de 10(4) UFC/g. Todas as amostras apresentaram ausência de Salmonella. CONCLUSÃO: A manutenção dos alimentos servidos, nos balcões de distribuição, em temperaturas abusivas e a presença de microrganismos indicadores de qualidade sanitária em número elevado comprometem a segurança e podem colocar em risco a saúde dos consumidores, em restaurantes self-service. Os instrumentos utilizados demonstraram-se como ferramentas importantes de avaliação da segurança das refeições servidas.<br>OBJECTIVE: This work aimed to assess the temperature and sanitary quality of the foods available on the buffet table of self-service restaurants located in the city of Taubaté (SP), in order to discuss food safety. METHODS: Four samples, two of cold dishes and two of hot dishes, were collected from each of 16 restaurants. The temperature of the foods on the buffet table was measured before the sample was taken. The following parameters were investigated for each sample: aerobic mesophilic bacteria count, determination of coliforms at 35ºC and 45ºC, coagulase-positive Staphylococcus count and presence of Salmonella. RESULTS: The temperature of 56 (87.5%) foods was inadequate: cold dishes were kept above 10ºC and hot dishes were kept below 60ºC. Aerobic mesophilic bacteria counts above 10(6) CFU/g were detected in 11% of the samples, coliforms at 35ºC in 76.6% and coliforms at 45ºC in 43.7%. Two foods, one cold and one hot, both from the same restaurant, had a coagulase-positive Staphylococcus count above 10(4) CFU/g. Salmonella was not found in any of the samples. CONCLUSION: Keeping the foods available on buffet tables under incorrect temperatures and a high count of microorganisms that indicate poor sanitary quality of the food can compromise safety and jeopardize the customer's health. The instruments used in this study proved to be important tools for assessing food safety

Increased Root Canal Endotoxin Levels are Associated with Chronic Apical Periodontitis, Increased Oxidative and Nitrosative Stress, Major Depression, Severity of Depression, and a Lowered Quality of Life

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Processo FAPESP: 2015/03696-1Evidence indicates that major depression is accompanied by increased translocation of gut commensal Gram-negative bacteria (leaky gut) and consequent activation of oxidative and nitrosative (O&NS) pathways. This present study examined the associations among chronic apical periodontitis (CAP), root canal endotoxin levels (lipopolysaccharides, LPS), O&NS pathways, depressive symptoms, and quality of life. Measurements included advanced oxidation protein products (AOPP), nitric oxide metabolites (NOx), lipid peroxides (LOOH), −sulfhydryl (SH) groups, total radical trapping antioxidant parameter (TRAP), and paraoxonase (PON)1 activity in participants with CAP, with and without depression, as well as healthy controls (no depression, no CAP). Root canal LPS levels were positively associated with CAP, clinical depression, severity of depression (as measured with the Hamilton Depression Rating Scale (HDRS) and the Beck Depression Inventory) and O&NS biomarkers, especially NOx and TRAP. CAP-related depression was accompanied by increased levels of NOx, LOOH, AOPP, and TRAP. In CAP participants, there was a strong correlation (r = 0.734, p OpenSPiltSPi 0.001) between root canal LPS and the HDRS score. There were significant and positive associations between CAP or root canal endotoxin with the vegetative and physio-somatic symptoms of the HDRS as well as a significant inverse association between root canal endotoxin and quality of life with strong effects on psychological, environmental, and social domains. It is concluded that increased root canal LPS accompanying CAP may cause depression and a lowered quality of life, which may be partly explained by activated O&NS pathways, especially NOx thereby enhancing hypernitrosylation and thus neuroprogressive processes. Dental health and “leaky teeth” may be intimately linked to the etiology and course of depression, while significantly impacting quality of life