Organospecific influence of the extract of cryopreserved piglets’ skin fragments

Researching mechanisms of peptides’ tissue-specific effect is an important task of modern molecular biology, physiology and medicine. In previous studies it was shown that extracts of cryopreserved piglets’ skin fragments and pigs’ spleen fragments accelerate and normalise the healing of skin wound in experiment. Fibroblasts culture is an appropriate model for studying tissue-specific biological activity of peptide complexes on relevant type of cells. This research was aimed to establish the influence of the extract of cryopreserved newborn piglets’ skin fragments (cNPSE) and the extract of cryopreserved pigs’ spleen fragments (cPSE) on proliferative and metabolic activity of skin fibroblasts in culture. Extracts were obtained from cryopreserved skin fragments and cryopreserved spleen fragments. Primary culture of neonatal rat skin fibroblasts was obtained by free cell transfer from skin fragments and subsequent reseeding. Metabolic activity of cells in culture was defined using non-toxic redox indicator AlamarBlue. The number of cells in a well was measured by counting the quantity of cells in wells. On the 7th day the metabolic activity of fibroblasts, cultured with cNPSE (1 μg/ml final peptide concentration), was higher than control by 1.3 times. Adding of cPSE (in the same concentration of peptides) increased cell metabolic activity by 1.2 times. While incubating fibroblasts in the medium with 2% FBS, a decrease of metabolic activity of cells was observed on the 5th day, and by the 7th day it was 51.2% of the control. At 1 and 1.5 μg/ml final peptide concentration of cNPSE, the metabolic activity of fibroblasts remained at the level observed in the control samples with 10% FBS. Adding cPSE to the incubation medium did not affect the metabolic activity of cells. Increased metabolic activity of cells (initially kept for 30 minutes at 4°C) was observed on the 5th and 7th day in cNPSE presence. Thus, it was found that adding cNPSE and cPSE to the culture medium of rats’ skin fibroblasts increase the metabolic activity of cells. A dose-dependent effect is observed. The addition of cNPSE to the medium with 2% FBS maintains the metabolic activity of fibroblasts at the level observed in the control samples incubated with 10% FBS. Adding extract also increases the metabolic activity of fibroblasts after hypothermic impact on the cells

Review: Electrochemiluminescence of Perovskite-Related Nanostructures

Perovskite nanostructures are promising nanomaterials for their possible application in electrochemiluminescent (ECL) analytical systems due to their unique optical, electronic, and chemical properties. This review focuses on the most recent advances in the application of perovskite and perovskite-related nanostructures, with different chemical compositions and modifications, in ECL with various media, coreactants, and reaction types. The most optimal methods of perovskite nanoparticle synthesis and electrode modification methods were reviewed. Possibilities and perspectives of the use of perovskite-related nanostructures for the ECL generation were demonstrated

Laser Synthesis of Cerium-Doped Garnet Nanoparticles

The application of a pulsed laser ablation technique for the generation of cerium-doped garnet nanoparticles in liquids is investigated. The morphological and optical properties of the obtained nanoparticles are demonstrated. Features introduced by the single crystals of Gd3Al2.4Ga2.6O12:Ce3+, Lu3Al5O12:Ce3+, and Y3Al1.25Ga3.75O12:Ce3+ from which the nanoparticles are generated, as well as the parameters of a liquid media on the garnet nanoparticle generation are experimentally studied using TEM and UV-Vis spectroscopy methods. It is shown how the size, shape, and internal structure of the nanoparticles are related to the external laser ablation conditions, as well as to the laser melting processes of NPs in the colloidal solutions. This work provides important information about the generated nanoparticles, which can be used as building blocks for specially designed structures with predetermined optical properties

Laser generation of CeAlO3 nanocrystals with perovskite structure

For the first time to the best of our knowledge, CeAlO3 nanocrystals with perovskite structure are synthesized by pulsed laser ablation technique. The morphological and optical properties of the obtained CeAlO3 nanocrystals are investigated. This work opens new prospects for the application of laser ablation methods for the generation of perovskite nanocrystals and development of novel nanocomposite structures, which can be applied for the fabrication of perovskite solar cells, scintillation detectors, catalysts, etc

Organospecific influence of the extract of cryopreserved piglets’ skin fragments

Researching mechanisms of peptides’ tissue-specific effect is an important task of modern molecular biology, physiology and medicine. In previous studies it was shown that extracts of cryopreserved piglets’ skin fragments and pigs’ spleen fragments accelerate and normalise the healing of skin wound in experiment. Fibroblasts culture is an appropriate model for studying tissue-specific biological activity of peptide complexes on relevant type of cells. This research was aimed to establish the influence of the extract of cryopreserved newborn piglets’ skin fragments (cNPSE) and the extract of cryopreserved pigs’ spleen fragments (cPSE) on proliferative and metabolic activity of skin fibroblasts in culture. Extracts were obtained from cryopreserved skin fragments and cryopreserved spleen fragments. Primary culture of neonatal rat skin fibroblasts was obtained by free cell transfer from skin fragments and subsequent reseeding. Metabolic activity of cells in culture was defined using non-toxic redox indicator AlamarBlue. The number of cells in a well was measured by counting the quantity of cells in wells. On the 7th day the metabolic activity of fibroblasts, cultured with cNPSE (1 μg/ml final peptide concentration), was higher than control by 1.3 times. Adding of cPSE (in the same concentration of peptides) increased cell metabolic activity by 1.2 times. While incubating fibroblasts in the medium with 2% FBS, a decrease of metabolic activity of cells was observed on the 5th day, and by the 7th day it was 51.2% of the control. At 1 and 1.5 μg/ml final peptide concentration of cNPSE, the metabolic activity of fibroblasts remained at the level observed in the control samples with 10% FBS. Adding cPSE to the incubation medium did not affect the metabolic activity of cells. Increased metabolic activity of cells (initially kept for 30 minutes at 4°C) was observed on the 5th and 7th day in cNPSE presence. Thus, it was found that adding cNPSE and cPSE to the culture medium of rats’ skin fibroblasts increase the metabolic activity of cells. A dose-dependent effect is observed. The addition of cNPSE to the medium with 2% FBS maintains the metabolic activity of fibroblasts at the level observed in the control samples incubated with 10% FBS. Adding extract also increases the metabolic activity of fibroblasts after hypothermic impact on the cells

Different Roles of Ce3+ Optical Centers in Oxyorthosilicate Nanocrystals at X-ray and UV Excitation

Luminescence properties of Lu2SiO5:Ce3+ and Y2SiO5:Ce3+ nanocrystals were studied using photo- and X-ray luminescence techniques. The crystal structure of Re2SiO5 nanocrystals (P21/c space group) differs from the crystal structure of Re2SiO5 bulk crystals (C2/c space group) with 9- and 7-oxygen-coordinated cation positions instead of 6- and 7-coordinated ones observed for Re2SiO5 bulk crystals. Two optical centers (Ce1 and Ce2) were observed for Re2SiO5:Ce3+ nanocrystals originating from cerium ions substituting 9- and 7-oxygen-coordinated cation sites. Preferential substitution of larger cation sites by cerium ions leads to higher photoluminescence intensity of Ce1 centers, however, Ce2 centers are the main centers for electron-hole recombination, so only Ce2 band is observed in X-ray luminescence spectra. The features of oxygen coordination of Ce1 and Ce2 centers and high content of oxygen vacancies in Re2SiO5:Ce3+ nanocrystals can provide preferential trapping of electrons near Ce2 centers, and therefore, the dominant role of Ce2 band in X-ray luminescence spectra

Cechy rehabilitacji osób z uszkodzeniami rdzenia kręgowego

The article shows the results of the study concerning the rehabilitation peculiarities of young people with cervical spinal cord injuries. The aim of the study is to develop the optimal rehabilitation program, motor development and motor compensation, physical characteristics, mental health, nerve and muscle function, and the formation of positive motivation during special training programs. It will help young people adapt to their new situation, and to integrate them into society. The results of the experiment have shown quite a low level of development of motor skills, manual motor skills, daily living skills, and little regulation of the visceral motor system. The aim of the present work is to present correctional rehabilitation during three phases (early, late, and residual). 150 students, including 90 boys and 60 girls aged 16 to 18 with incomplete cervical spinal cord injuries participated in the study. It has been proven that the main psychological and pedagogical conditions, which fully ensure the effectiveness of psychophysical and social rehabilitation of elder students with incomplete cervical spinal cord injuries are: staged approach to rehabilitation that takes into account the complexity of this disease and personal characteristics, creating comfortable conditions and situations that form optimistic expectations about students’ capabilities, and positive didactic rehabilitation motivation; a comprehensive approach to the correction using psychological and social factors, specific motor training, methods of control, motor skills formation, physical development, correction, and motor compensation; control of the level of fundamental motor skills; a comprehensive approach to social adaptation; and finally high physical activity level of each person with disabilities in official and independent types of physical therapy. Specially organized training according to all the criteria and indicators, positively influenced the students from the experimental group in contrast to the control group, where the indicators remained quite low.W artykule przedstawiono wyniki badań dotyczących specyfiki rehabilitacji młodzieży po urazach rdzenia kręgowego w odcinku szyjnym. Celem pracy jest przygotowanie optymalnego programu rehabilitacji, związanego z rozwojem motorycznym i kompensacją ruchową, cechami fizycznymi, stanem psychicznym, funkcjonowaniem nerwów i mięśni, a także kształtowanie pozytywnej motywacji podczas specjalnych programów treningowych, co pomoże przystosować się do nowej sytuacji, przyciągnąć i zintegrować ze społeczeństwem. Wyniki eksperymentu ustalającego wykazały dość niski poziom rozwoju motoryki, regulacji trzewnego układu motorycznego, motoryki manualnej i umiejętności życia codziennego. Istotą pracy eksperymentalnej jest rehabilitacja korekcyjna w trzech fazach (wczesna, późna, rezydualna). W badaniu wzięło udział 150 uczniów, w tym 90 chłopców i 60 dziewcząt w wieku od 16 do 18 lat, z niepełnymi uszkodzeniami rdzenia kręgowego w odcinku szyjnym. Eksperymentalnie udowodniono, że głównymi warunkami psychologicznymi i pedagogicznymi, które w pełni zapewniają skuteczność rehabilitacji psychofizycznej i społecznej uczniów w podanym wieku, z niepełnym uszkodzeniem rdzenia kręgowego w odcinku szyjnym są: tworzenie komfortowych warunków i sytuacji, które kształtują optymistyczne oczekiwania co do swoich możliwości oraz pozytywną motywację do rehabilitacji dydaktycznej; kompleksowe podejście do korekcji z wykorzystaniem czynników psychologicznych i społecznych, specyficzny trening motoryczny, metody kontroli i kształtowania zdolności motorycznych, rozwój fizyczny, korekcja i kompensacja ruchowa; kontrola poziomu podstawowych umiejętności motorycznych; kompleksowe podejście do adaptacji społecznej; wysoki poziom aktywności fizycznej każdej osoby niepełnosprawnej w oficjalnych i niezależnych formach fizjoterapii. Specjalnie zorganizowane szkolenie, według wszystkich kryteriów i wskaźników, pozytywnie wpłynęło na uczniów z grupy eksperymentalnej, w przeciwieństwie do grupy kontrolnej, w której wskaźniki pozostały na dość niskim poziomie

Review: Electrochemiluminescence of Perovskite-Related Nanostructures

Perovskite nanostructures are promising nanomaterials for their possible application in electrochemiluminescent (ECL) analytical systems due to their unique optical, electronic, and chemical properties. This review focuses on the most recent advances in the application of perovskite and perovskite-related nanostructures, with different chemical compositions and modifications, in ECL with various media, coreactants, and reaction types. The most optimal methods of perovskite nanoparticle synthesis and electrode modification methods were reviewed. Possibilities and perspectives of the use of perovskite-related nanostructures for the ECL generation were demonstrated

Professional Training of Future Music Teachers in Ukraine in the Conditions of Martial Law: Problems and Prospects

У статті окреслено проблеми та перспективи професійної підготовки майбутніх учителів музичного мистецтва в умовах воєнного стану. Серед проблем визначено такі, як: руйнування та пошкодження закладів вищої освіти України, масштабне переміщення населення, вимушене перебування на тимчасово окупованих територіях, погіршення психоемоційного стану учасників освітнього процесу, складність організації освітнього процесу в дистанційному форматі та неможливість повноцінного доступу до навчання, неможливість створення безпечних умов для організації освітнього процесу в будь‐якій формі, зниження мотивації навчання через умови постійної невизначеності, нестабільний психоемоційний стан і часті сповіщення про повітряну тривогу, а для деяких учасників освітнього процесу, які перебувають у зоні активних воєнних дій, – обстрілів. Автором запропоновано можливі перспективи навчання майбутніх учителів музичного мистецтва в Україні в умовах воєнного часу, як‐от: активізація проведення індивідуальних музичних занять за спеціалізаціями у форматі реального часу з використанням програм Zoom, Google Classroom та Google Meet; використання практики показу відео‐ занять як викладачів університету, так і запрошених лекторів та відомих митців; застосування сучасних інформаційних технологій, які дають змогу відкрити нові перспективи для підвищення якості освітнього процесу (програмні розробки, цифрові сервіси та платформи, мультимедійні засоби); залучення здобувачів вищої освіти до науково‐дослідницької роботи; участь студентів у мистецьких конкурсах, фестивалях різного рівня; залучення до здійснення концертно‐просвітницької діяльності.The article outlines the problems and prospects of professional training of future music teachers in martial law. Problems are identified as: destruction and damage to higher education institutions of Ukraine, large‐scale movement of the population, forced stay in temporarily occupied territories, deterioration of the psycho‐ emotional state of participants in the educational process, complexity of organizing the educational process in remote format and impossibility conditions for the organization of the educational process in any form, reducing the motivation of learning due to the conditions of constant uncertainty, unstable psycho‐emotional state and frequent notifications of air alarm, and for some participants of the educational process in the area of active hostilities‐shelling. The author suggests possible prospects for teaching future music teachers in Ukraine in wartime, such as: activation of individual music classes in real‐time specializations using Zoom, Google Classroom and Google Meet; the use of video shows by both teachers of the university and invited lecturers and famous artists; application of modern information technologies that allow you to open new prospects to improve the quality of the educational process (software development, digital services and platforms, multimedia tools); involvement of higher education applicants in research work; participation of students in art competitions, festivals of different levels; involvement in concert and educational activities