80 research outputs found

    Laser cross-linking protein captures for living cells on a biochip

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    ANISOTROPIC GOLD NANOCRYSTALS: SYNTHESIS AND CHARACTERIZATION

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    In this letter we report on successful preparation and characterization of anisotropic gold nanocrystals bio-synthesized by reduction of aqueous chloroaurate ions in pelargonium plant extract. The nanocrystals have been characterized by means of Transmission Electron Microscopy (TEM), UV-VIS absorption spectroscopy and tapping mode atomic force microscopy (TM-AFM). Using these investigation techniques, the successful formation of anisotropic single nanocrystals with the preferential growth direction along the gold (111) plane has been confirmed. The high detail phase images could give us an explanation concerning the growth mechanism of the nanocrystals.</jats:p

    Microstructuration of protein matrices by laser-induced photochemistry

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    International audienceWe report on the laser fabrication of BSA and type I collagen microstructures using Eosin Y or Rose Bengal photo sensitizers. Similar cross-linking results have been obtained by one-photon absorption or two-photon absorption using a green Q-switched microlaser or a near-infrared mode-locked Ti:sapphire laser, respectively. The microstructure resolution depends on the laser power, but is typically limited by the size of the laser beam within the sample. The cross-linking of type I collagen with Rose Bengal is obtained in acidic conditions with a large photosensitizer concentration in the millimole range. The biocompatibility of microstructures was demonstrated by observing the specific adhesion of cells on collagen lines
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