Potential of new isolates of Dunaliella Salina for natural β-Carotene production

The halotolerant microalga Dunaliella salina has been widely studied for natural β-carotene production. This work shows biochemical characterization of three newly isolated Dunaliella salina strains, DF15, DF17, and DF40, compared with D. salina CCAP 19/30 and D. salina UTEX 2538 (also known as D. bardawil). Although all three new strains have been genetically characterized as Dunaliella salina strains, their ability to accumulate carotenoids and their capacity for photoprotection against high light stress are different. DF15 and UTEX 2538 reveal great potential for producing a large amount of β-carotene and maintained a high rate of photosynthesis under light of high intensity; however, DF17, DF40, and CCAP 19/30 showed increasing photoinhibition with increasing light intensity, and reduced contents of carotenoids, in particular β-carotene, suggesting that the capacity of photoprotection is dependent on the cellular content of carotenoids, in particular β-carotene. Strong positive correlations were found between the cellular content of all-trans β-carotene, 9-cis β-carotene, all-trans α-carotene and zeaxanthin but not lutein in the D. salina strains. Lutein was strongly correlated with respiration in photosynthetic cells and strongly related to photosynthesis, chlorophyll and respiration, suggesting an important and not hitherto identified role for lutein in coordinated control of the cellular functions of photosynthesis and respiration in response to changes in light conditions, which is broadly conserved in Dunaliella strains. Statistical analysis based on biochemical data revealed a different grouping strategy from the genetic classification of the strains. The significance of these data for strain selection for commercial carotenoid production is discussed

Management of late-preterm and term infants with hyperbilirubinaemia in resource-constrained settings.

Hyperbilirubinaemia is a ubiquitous transitional morbidity in the vast majority of newborns and a leading cause of hospitalisation in the first week of life worldwide. While timely and effective phototherapy and exchange transfusion are well proven treatments for severe neonatal hyperbilirubinaemia, inappropriate or ineffective treatment of hyperbilirubinaemia, at secondary and tertiary hospitals, still prevails in many poorly-resourced countries accounting for a disproportionately high burden of bilirubin-induced mortality and long-term morbidity. As part of the efforts to curtail the widely reported risks of frequent but avoidable bilirubin-induced neurologic dysfunction (acute bilirubin encephalopathy (ABE) and kernicterus) in low and middle-income countries (LMICs) with significant resource constraints, this article presents a practical framework for the management of late-preterm and term infants (≥ 35 weeks of gestation) with clinically significant hyperbilirubinaemia in these countries particularly where local practice guidelines are lacking. Standard and validated protocols were followed in adapting available evidence-based national guidelines on the management of hyperbilirubinaemia through a collaboration among clinicians and experts on newborn jaundice from different world regions. Tasks and resources required for the comprehensive management of infants with or at risk of severe hyperbilirubinaemia at all levels of healthcare delivery are proposed, covering primary prevention, early detection, diagnosis, monitoring, treatment, and follow-up. Additionally, actionable treatment or referral levels for phototherapy and exchange transfusion are proposed within the context of several confounding factors such as widespread exclusive breastfeeding, infections, blood group incompatibilities and G6PD deficiency, which place infants at high risk of severe hyperbilirubinaemia and bilirubin-induced neurologic dysfunction in LMICs, as well as the limited facilities for clinical investigations and inconsistent functionality of available phototherapy devices. The need to adjust these levels as appropriate depending on the available facilities in each clinical setting and the risk profile of the infant is emphasised with a view to avoiding over-treatment or under-treatment. These recommendations should serve as a valuable reference material for health workers, guide the development of contextually-relevant national guidelines in each LMIC, as well as facilitate effective advocacy and mobilisation of requisite resources for the optimal care of infants with hyperbilirubinaemia at all levels

Many worlds and modality in the interpretation of quantum mechanics: an algebraic approach

Many worlds interpretations (MWI) of quantum mechanics avoid the measurement problem by considering every term in the quantum superposition as actual. A seemingly opposed solution is proposed by modal interpretations (MI) which state that quantum mechanics does not provide an account of what `actually is the case', but rather deals with what `might be the case', i.e. with possibilities. In this paper we provide an algebraic framework which allows us to analyze in depth the modal aspects of MWI. Within our general formal scheme we also provide a formal comparison between MWI and MI, in particular, we provide a formal understanding of why --even though both interpretations share the same formal structure-- MI fall pray of Kochen-Specker (KS) type contradictions while MWI escape them.Comment: submitted to the Journal of Mathematical Physic

Real-time volumetric lipid imaging in vivo by intravascular photoacoustics at 20 frames per second

Lipid deposition can be assessed with combined intravascular photoacoustic/ultrasound (IVPA/US) imaging. To date, the clinical translation of IVPA/US imaging has been stalled by a low imaging speed and catheter complexity. In this paper, we demonstrate imaging of lipid targets in swine coronary arteries in vivo, at a clinically useful frame rate of 20 s−1. We confirmed image contrast for atherosclerotic plaque in human samples ex vivo. The system is on a mobile platform and provides real-time data visualization during acquisition. We achieved an IVPA signal-to-noise ratio of 20 dB. These data show that clinical translation of IVPA is possible in principle

Enhancing accuracy and precision of transparent synthetic soil modelling

Over recent years non-intrusive modelling techniques have been developed to investigate soil-structure interaction problems of increasingly complex geometry. This paper concerns the development of a small-scale, 1 g, modelling technique using a transparent analogue for soil with particle image velocimetry for internal displacement measurement. Larger model geometry achieved in this research using fine-grained transparent synthetic soils has led to an increased need for rigorous photogrammetric correction techniques. A correction framework, based upon a modified version of the pinhole camera model, is presented that corrects for lens and camera movement induced errors as well as scaling from image space to object space. An additional statistical approach is also developed to enhance the system precision, by minimising the impact of increased non-coplanarity between the photogrammetry control plane and the target plane. The enhanced data correction and statistical precision is demonstrated using a case study examining the failure mechanism around a double helical screw pile installed in transparent synthetic soil representative of a soft clay

Women in engineering: statistical analysis of ACT data and proposed procedure to reverse trend

Journal ArticleWomen have historically been underrepresented both in college majors and professional careers in STEM fields. This disparity can be observed in many countries, though it is most evident in the US. In this paper we analyze historical ACT data over a 30-year span and correlate gender differences with ACT scores and expressed interest in STEM (Science, Technology, Engineering, and Math) related college majors. SPSS software was used to analyze the data and examine the historical trends of students' expressed interest in STEM related majors. Results show that there is a significant discrepancy between the number of men and women students who expressed interest in engineering majors. The data also show that social influences such as the emergence of computer fields including computer gaming and the dot.com era have profound influence in students' interest in STEM fields. To help develop specific strategies for timely remediation and help specific strategies for timely remediation and help reverse this trend students were grouped into three categories -- well prepared (ACT ? 28), under prepared (27 ? ACT ? 19), and unprepared (ACT < 19). Of the total number of students who expressed interest in engineering majors there are many who appear either completely unprepared or relatively under prepared for the demands of these fields. Results from this analysis demonstrate the importance of earlier interventions to encourage students who still have enough time to prepare for opportunities that interest them. It is also probable that students are making college major choices based on little or no data, and may, therefore, be at retention risk if they are admitted into an engineering program. This paper also highlights ongoing efforts to share data and work with high school counselors in an effort to help students identify more realistic career options or to timely target students for effective math remediation and help encourage increased participation in STEM majors and careers. Based on the data found in these analyses, we will next be surveying high school career guidance counselors. This survey will help gain insight into the high school counselors' understanding and potential biases regarding engineering and engineers

Update of Guidelines for laparoscopic treatment of ventral and incisional abdominal wall hernias (International Endohernia Society (IEHS)) : Part B

In 2014 the International Endohernia Society (IEHS) published the first international "Guidelines for laparoscopic treatment of ventral and incisional abdominal wall hernias". Guidelines reflect the currently best available evidence in diagnostics and therapy and give recommendations to help surgeons to standardize their techniques and to improve their results. However, science is a dynamic field which is continuously developing. Therefore, guidelines require regular updates to keep pace with the evolving literature. Methods For the development of the original guidelines all relevant literature published up to year 2012 was analyzed using the ranking of the Oxford Centre for Evidence-Based-Medicine. For the present update all of the previous authors were asked to evaluate the literature published during the recent years from 2012 to 2017 and revise their statements and recommendations given in the initial guidelines accordingly. In two Consensus Conferences (October 2017 Beijing, March 2018 Cologne) the updates were presented, discussed, and confirmed. To avoid redundancy, only new statements or recommendations are included in this paper. Therefore, for full understanding both of the guidelines, the original and the current, must be read. In addition, the new developments in repair of abdominal wall hernias like surgical techniques within the abdominal wall, release operations (transversus muscle release, component separation), Botox application, and robot-assisted repair methods were included. Results Due to an increase of the number of patients and further development of surgical techniques, repair of primary and secondary abdominal wall hernias attracts increasing interests of many surgeons. Whereas up to three decades ago hernia-related publications did not exceed 20 per year, currently this number is about 10-fold higher. Recent years are characterized by the advent of new techniques-minimal invasive techniques using robotics and laparoscopy, totally extraperitoneal repairs, novel myofascial release techniques for optimal closure of large defects, and Botox for relaxing the abdominal wall. Furthermore, a concomitant rectus diastasis was recognized as a significant risk factor for recurrence. Despite still insufficient evidence with respect to these new techniques it seemed to us necessary to include them in the update to stimulate surgeons to do research in these fields. Conclusion Guidelines are recommendations based on best available evidence intended to help the surgeon to improve the quality of his daily work. However, science is a continuously evolving process, and as such guidelines should be updated about every 3 years. For a comprehensive reference, however, it is suggested to read both the initially guidelines published in 2014 together with the update. Moreover, the presented update includes also techniques which were not known 3 years before

Three-dimensional electromagnetic power deposition in tumors using interstitial antenna arrays

Journal ArticleInterstitial arrays of insulated antennas have shown promise for microwave hyperthermia treatment of deep-seated tumors. Available analytical techniques for predicting the electromagnetic (EM) power deposition of these antennas have been limited to the case of a homogeneous conductive medium surrounding the array. Since tumors and host tissue may differ in their electrical characteristics, it is necessary to consider the impact of this variation in electrical properties and the geometry of the tumor in the calculation of the EM field distribution and power deposition pattern when modeling interstitial antennas

Gender differences in expressed interests in engineering-related fields over a 30-year span

Journal ArticleThis study examines gender differences and historical trends of high school student interest in engineering based on ACT data on expressed interest compared with that of students' and ability. Changes have been observed in the interest in engineering fields over time most likely because of societal influences. These influences are especially seen in computer related fields causing speculation that both males and females were influenced by the dot com era but that only male interest was piqued due to the rise of computer games in the late 1990's. Another interesting observation is the number of students stating they are interested in engineering careers but who minimally or poorly prepared based on their ACT math scores. This raises the question of whether these students understand what engineering is, and whether they have been informed of the demands of the major. These students are likely to face retention issues in engineering fields and are often candidates for math remediation. By better understanding the societal influences as well as gender and ability disparities we will have a better understanding of what needs to be done in order to reverse the current trends of gender disparity and lack of interest in engineering fields

Agrometerological study of semi-arid areas : an experiment for analysing the potential of time series of FORMOSAT-2 images (Tensift-Marrakech plain)

Earth Observing Systems designed to provide both high spatial resolution (10m) and high capacity of time revisit (a few days) offer strong opportunities for the management of agricultural water resources. The FORMOSAT-2 satellite is the first and only satellite with the ability to provide daily high-resolution images over a particular area with constant viewing angles. As part of the SudMed project, one of the first time series of FORMOSAT-2 images has been acquired over the semi-arid Tensift-Marrakech plain. Along with these acquisitions, an experimental data set has been collected to monitor land-cover/land-use, soil characteristics, vegetation dynamics and surface fluxes. This paper presents a first analysis of the potential of these data for agrometerological study of semi-arid areas