Pengaruh Dukungan Kelompok Terhadap Kualitas Hidup Penderita Diabetes Melitus Di Persadia Cabang Surakarta

Diabetes mellitus is a major health problem. Diabetes mellitus can be serious and lead to chronic conditions that endanger if not treated. It gives effect to the quality of life of patients with DM. Interviews showed that Persadia members active in Surakarta branch has a health condition and quality of life is further improved compared with DM patients who are not actively participating or not at all health conditions and quality of life to decrease. This study aimed to analyze the influence of support groups on quality of life in patients with DM Surakarta branch Persadia. This study was a correlational analytical survey. The samples are 74 people with diabetes mellitus who were active in Persadia with the determination of purposive sampling techniques. The date collection used questionnaires. Data analysis was performed using univariate and bivariate analysis. The study showed that (1) average price in DM patients support groups active in Persadia Surakarta branch of 66.19, (2) the average quality of life of people with diabetes who were active in Persadia Surakarta branch of 88.51, and (3) there was a positive relationship between the group support with quality of life in patients with DM in Surakarta branch Persadia (p-value = 0.017), hence, the better the group support, the quality of life of people with DM is increasing

Leaf-applied sodium chloride promotes cadmium accumulation in durum wheat grain

Cadmium (Cd) accumulation in durum wheat grain is a growing concern. Among the factors affecting Cd accumulation in plants, soil chloride (Cl) concentration plays a critical role. The effect of leaf NaCl application on grain Cd was studied in greenhouse-grown durum wheat (Triticum turgidum L. durum, cv. Balcali-2000) by immersing (10 s) intact flag leaves into Cd and/or NaCl-containing solutions for 14 times during heading and dough stages. Immersing flag leaves in solutions containing increasing amount of Cd resulted in substantial increases in grain Cd concentration. Adding NaCl alone or in combination with the Cd-containing immersion solution promoted accumulation of Cd in the grains, by up to 41%. In contrast, Zn concentrations of grains were not affected or even decreased by the NaCl treatments. This is likely due to the effect of Cl complexing Cd and reducing positive charge on the metal ion, an effect that is much smaller for Zn. Charge reduction or removal (CdCl2 0 species) would increase the diffusivity/lipophilicity of Cd and enhance its capability to penetrate the leaf epidermis and across membranes. Of even more significance to human health was the ability of Cl alone to penetrate leaf tissue and mobilize and enhance shoot Cd transfer to grains, yet reducing or not affecting Zn transfer

IFNAR1-Signalling Obstructs ICOS-mediated Humoral Immunity during Non-lethal Blood-Stage Plasmodium Infection

Funding: This work was funded by a Career Development Fellowship (1028634) and a project grant (GRNT1028641) awarded to AHa by the Australian National Health & Medical Research Council (NHMRC). IS was supported by The University of Queensland Centennial and IPRS Scholarships. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

G-quadruplex-binding small molecules ameliorate C9orf72 FTD/ALS pathology in vitro and in vivo

Intronic GGGGCC repeat expansions in C9orf72 are the most common known cause of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS), which are characterised by degeneration of cortical and motor neurons, respectively. Repeat expansions have been proposed to cause disease by both the repeat RNA forming foci that sequester RNA-binding proteins and through toxic dipeptide repeat proteins generated by repeat-associated non-ATG translation. GGGGCC repeat RNA folds into a G-quadruplex secondary structure, and we investigated whether targeting this structure is a potential therapeutic strategy. We performed a screen that identified three structurally related small molecules that specifically stabilise GGGGCC repeat G-quadruplex RNA We investigated their effect in C9orf72 patient iPSC-derived motor and cortical neurons and show that they significantly reduce RNA foci burden and the levels of dipeptide repeat proteins. Furthermore, they also reduce dipeptide repeat proteins and improve survival in vivo, in GGGGCC repeat-expressing Drosophila Therefore, small molecules that target GGGGCC repeat G-quadruplexes can ameliorate the two key pathologies associated with C9orf72 FTD/ALS These data provide proof of principle that targeting GGGGCC repeat G-quadruplexes has therapeutic potential

Antiangiogenic Effect of Ficus deltoidea Jack Standardised Leaf Extracts

Purpose: To standardise the methanol and aqueous extracts of Ficus deltoidea leaf by developing a reverse phase high performance liquid chromatography (RP-HPLC) for determination of the ursolic acid content and to investigate their antiangiogenic activity.Methods: To prepare the water extract (FD-A), the powder of the plant was extracted with water under reflux for 24 h at 50 ˚C. The methanol extract (FD-M) was prepared using Soxhlet extractor for 24 h at 50 ˚C. The extracts were standardised for ursolic acid content by reverse phase high performance liquid chromatography (RP-HPLC), as well as by total phenolic and flavonoid contents. Antiangiogenic activity was studied using ex vivo rat aortic rings and in vivo chick chorioallantoic membrane angiogenesis models. The anti-proliferative effect of the extracts against normal human endothelial cells and two cancer cell lines was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.Results: In rat aortic rings, methanol and water extracts inhibited the outgrowth of microvessels with IC50 values of 48.2 ± 1.1 and 62.7 ± 1.4 μg ml-1, respectively. Methanol and water extracts at doses of 100 μg disc-1 also inhibited vascularisation of chick embryo chorioallantoic membrane with inhibition values of of 62.0 ± 6.3 and 43.3 ± 4.8 %, respectively. In addition, both extracts showed potent cytotoxicity against breast and colon cancer cells while demonstrating non-cytotoxic activity against normal endothelial cells. Furthermore, the two extracts showed relatively high concentration of ursolic acid, total phenolics and flavonoids associated with potent antiangiogenic activity.Conclusion: The inhibition of angiogenesis by F. deltoidea extracts may be attributed to the relatively high ursolic acid content as well as the presence of antioxidant compounds of phenolics and flavonoids in the extracts.Keywords: Ficus deltoidea, Antiangiogenesis, Ursolic acid, Cytotoxicity, Antioxidan

Evaluation Of The Essential Oil Of Foeniculum Vulgare Mill (Fennel) Fruits Extracted By Three Different Extraction Methods By Gc/Ms

Background: Hydrodistillation (HD) and steam-distillation, or solvent extraction methods of essential oils have some disadvantages like thermal decomposition of extracts, its contamination with solvent or solvent residues and the pollution of residual vegetal material with solvent which can be also an environmental problem. Thus, new green techniques, such as supercritical fluid extraction and microwave assisted techniques, are potential solutions to overcome these disadvantages.Materials and Methods: The aim of this study was to evaluate the essential oil of Foeniculum vulgare subsp. Piperitum fruits extracted by three different extraction methods viz. Supercritical fluid extraction (SFE) using CO2, microwave-assisted extraction (MAE) and hydro-distillation (HD) using gas chromatography-mass spectrometry (GC/MS).Results: The results revealed that both MAE and SFE enhanced the extraction efficiency of the interested components. MAE gave the highest yield of oil as well as higher percentage of Fenchone (28%), whereas SFE gave the highest percentage of anethol (72%).Conclusion: Microwave-assisted extraction (MAE) and supercritical fluid extraction (SFE) not only enhanced the essential oil extraction but also saved time, reduced the solvents use and produced, ecologically, green technologies.Keywords: Foeniculum vulgare subsp. Piperitum, microwave-assisted extraction (MAE), Supercritical fluid extraction (SFE), Essential Oils,Anethol, Fenchone, Limonene, Pinene, GC/MS

Literatur review: diet dan tekanan darah pada pasien hipertensi

Hipertensi adalah penyakit tidak menular terbanyak di dunia. Hipertensi merupakan kondisi tekanan darah yang berada di atas normal yaitu siastolik ? 140 mmHg dan diastolik ? 90 mmHg. Tekanan darah sistolik adalah tekanan saat jantung memompa darah ke seluruh tubuh. Tekanan darah diastolik adalah tekanan terendah di antara kontraksi (jantung beristirahat ketika jantung dalam keadaan rileks sambil mengisi ulang biliknya dengan darah). Penelitian ini bertujuan untuk menganalisis persamaan ,pada perbedaan referensi atau jurnal yang dipilih untuk diketahui tentang diet dan tekanan darah pada pasien hipertensi. Jenis pada metode penelitian ini menggunakan literature review.Pencarian jurnal dilakukan pada portal jurnal online seperti Google Scholar,PubMed. Hasil penelusuran jurnal didaptkan 7 jurnal pada penelitian ini. Hasil keseluruhan pencarian 2 database terdapat 20.065 artikel. Setelah di screening judul dan relevan abstrak diperoleh 7 jurnal yang membuktikan bahwa adanya diet dan tekanan darah pada pasien hipertensi. Hasil dari 7 artikel yang telah dilakukan review seluruhnya membuktikan bahwa pola diet terbukti efektif dalam menurunkan tekanan darah pada pasien hipertensi. Modifikasi diet atau pengaturan diet sangat penting bagi penderita hipertensi,selain itu tujuan utama dari pengaturan diet hipertensi ialah mengatur tentang makanan sehat yang dapat mengontrol tekanan darah tinggi dan mengurangi penyakit kardiovaskuler

Influence of Stefan blowing on nanofluid flow submerged in microorganisms with leading edge accretion or ablation

The unsteady forced convective boundary layer flow of viscous incompressible fluid containing both nanoparticles and gyrotactic microorganisms, from a flat surface with leading edge accretion (or ablation), is investigated theoretically. Utilizing appropriate similarity transformations for the velocity, temperature, nanoparticle volume fraction and motile microorganism density, the governing conservation equations are rendered into a system of coupled, nonlinear, similarity ordinary differential equations. These equations, subjected to imposed boundary conditions, are solved numerically using the Runge-Kutta-Fehlberg fourth-fifth order numerical method in the MAPLE symbolic software. Good agreement between our computations and previous solutions is achieved. The effect of selected parameters on flow velocity, temperature, nano-particle volume fraction (concentration) and motile microorganism density function is investigated. Furthermore, tabular solutions are included for skin friction, wall heat transfer rate, nano-particle mass transfer rate and microorganism transfer rate. Applications of the study arise in advanced micro-flow devices to assess nanoparticle toxicity