Protein separation using membrane-encapsulated soluble ligand conjugates

A new approach for isolating and recovering biological macromolecules using membrane-encapsulated soluble ligand conjugates was investigated. Membrane-encapsulated solid adsorbents have been successfully developed and employed in our laboratory to isolate and purify proteins and enzymes directly from culture broths. This new concept also makes it possible to use soluble ligand conjugates instead of solid adsorbents inside membrane capsules. In this work, model membrane-encapsulated soluble and insoluble ligands comprising Blue Dextran and Blue Sepharose entrapped within calcium alginate membranes were studied to compare adsorption characteristics such as capacities and rates. Experimental results suggest that membrane-encapsulated soluble ligands may be expected to result in higher overall adsorption capacity compared to membrane-encapsulated solid adsorbents with comparable adsorption rates.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/28604/1/0000413.pd

A new isolation and purification method for staphylococcal protein A using membrane encapsulated rabbit IgG-agarose

A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG-agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/37901/1/260340811_ftp.pd

Plugging the drain : promoting environmental justice in the Niger Delta through judicial independence

This thesis highlights the multiple forms of injustice that are experienced in the Niger Delta using the concept of environmental justice. It discusses prominent cases such as Gbemre v. Shell and Bodo v. Shell and identifies judicial independence as a crucial component of attaining justice for Niger Deltans. Using the branches of environmental justice, it explains the risks and harms that have occurred within this region and the kinds of remedies that are required for an improvement. In particular, it reveals disproportionate arrangements in the shares of benefits and burdens of the environmental resources in the country and highlights several social and political arrangements which promote these disparities. The thesis further highlights the need for a remedy and the reasons why these remedies have been far-fetched and this leads to the discussion about the best ways to achieve these remedies. In considering the best ways forward from the environmental injustice, this thesis discusses the topic of judicial independence, which it highlights as a crucial factor for the attainment of environmental justice. It views this as a plug to a drain, which ensures that the good efforts which are made towards the attainment of environmental justice, in the form of reformed laws and better processes amongst others, do not go to waste. Conversely, it argues that the lack of judicial independence undoes any good efforts and frustrates the attempts of the Niger Deltans to attain environmental justice. An independent, impartial and competent court is therefore shown to be an essential requirement, if environmental justice is to be achieved and sustained in the Niger Delta

Plugging the drain : promoting environmental justice in the Niger Delta through judicial independence

This thesis highlights the multiple forms of injustice that are experienced in the Niger Delta using the concept of environmental justice. It discusses prominent cases such as Gbemre v. Shell and Bodo v. Shell and identifies judicial independence as a crucial component of attaining justice for Niger Deltans. Using the branches of environmental justice, it explains the risks and harms that have occurred within this region and the kinds of remedies that are required for an improvement. In particular, it reveals disproportionate arrangements in the shares of benefits and burdens of the environmental resources in the country and highlights several social and political arrangements which promote these disparities. The thesis further highlights the need for a remedy and the reasons why these remedies have been far-fetched and this leads to the discussion about the best ways to achieve these remedies. In considering the best ways forward from the environmental injustice, this thesis discusses the topic of judicial independence, which it highlights as a crucial factor for the attainment of environmental justice. It views this as a plug to a drain, which ensures that the good efforts which are made towards the attainment of environmental justice, in the form of reformed laws and better processes amongst others, do not go to waste. Conversely, it argues that the lack of judicial independence undoes any good efforts and frustrates the attempts of the Niger Deltans to attain environmental justice. An independent, impartial and competent court is therefore shown to be an essential requirement, if environmental justice is to be achieved and sustained in the Niger Delta

Plugging the drain : promoting environmental justice in the Niger Delta through judicial independence

This thesis highlights the multiple forms of injustice that are experienced in the Niger Delta using the concept of environmental justice. It discusses prominent cases such as Gbemre v. Shell and Bodo v. Shell and identifies judicial independence as a crucial component of attaining justice for Niger Deltans. Using the branches of environmental justice, it explains the risks and harms that have occurred within this region and the kinds of remedies that are required for an improvement. In particular, it reveals disproportionate arrangements in the shares of benefits and burdens of the environmental resources in the country and highlights several social and political arrangements which promote these disparities. The thesis further highlights the need for a remedy and the reasons why these remedies have been far-fetched and this leads to the discussion about the best ways to achieve these remedies. In considering the best ways forward from the environmental injustice, this thesis discusses the topic of judicial independence, which it highlights as a crucial factor for the attainment of environmental justice. It views this as a plug to a drain, which ensures that the good efforts which are made towards the attainment of environmental justice, in the form of reformed laws and better processes amongst others, do not go to waste. Conversely, it argues that the lack of judicial independence undoes any good efforts and frustrates the attempts of the Niger Deltans to attain environmental justice. An independent, impartial and competent court is therefore shown to be an essential requirement, if environmental justice is to be achieved and sustained in the Niger Delta.Law, Peter A. Allard School ofGraduat