Universal computing by DNA origami robots in a living animal

Biological systems are collections of discrete molecular objects that move around and collide with each other. Cells carry out elaborate processes by precisely controlling these collisions, but developing artificial machines that can interface with and control such interactions remains a significant challenge. DNA is a natural substrate for computing and has been used to implement a diverse set of mathematical problems1-3, logic circuits4-6 and robotics7-9. The molecule also naturally interfaces with living systems, and different forms of DNA-based biocomputing have previously been demonstrated10-13. Here we show that DNA origami14-16 can be used to fabricate nanoscale robots that are capable of dynamically interacting with each other17-18 in a living animal. The interactions generate logical outputs, which are relayed to switch molecular payloads on or off. As a proof-of-principle, we use the system to create architectures that emulate various logic gates (AND, OR, XOR, NAND, NOT, CNOT, and a half adder). Following an ex vivo prototyping phase, we successfully employed the DNA origami robots in living cockroaches (Blaberus discoidalis) to control a molecule that targets the cells of the animal

Effect of methotrexate and anti-TNF on Epstein-Barr virus T-cell response and viral load in patients with rheumatoid arthritis or spondylarthropathies

INTRODUCTION: There is a suspicion of increased risk of Epstein-Barr virus (EBV)-associated lymphoproliferations in patients with inflammatory arthritides receiving immunosuppressive drugs. We investigated the EBV load and EBV-specific T-cell response in patients treated with methotrexate (MTX) or anti-TNF therapy. METHODS: Data for patients with rheumatoid arthritis (RA) (n = 58) or spondylarthropathy (SpA) (n = 28) were analyzed at baseline in comparison with controls (n = 22) and after 3 months of MTX or anti-TNF therapy for EBV load and EBV-specific IFNγ-producing T cells in response to EBV latent-cycle and lytic-cycle peptides. RESULTS: The EBV load and the number of IFNγ-producing T-cells after peptide stimulation were not significantly different between groups at baseline (P = 0.61 and P = 0.89, respectively). The EBV load was not significantly modified by treatment, for RA with MTX (P = 0.74) or anti-TNF therapy (P = 0.94) or for SpA with anti-TNF therapy (P = 1.00). The number of EBV-specific T cells was not significantly modified by treatment, for RA with MTX (P = 0.58) or anti-TNF drugs (P = 0.19) or for SpA with anti-TNF therapy (P = 0.39). For all patients, the EBV load and EBV-specific T cells were significantly correlated (P = 0.017; R = 0.21). For most patients, short-term exposure (3 months) to MTX or anti-TNF did not alter the EBV load or EBV-specific T-cell response but two patients had discordant evolution. CONCLUSIONS: These data are reassuring and suggest there is no short-term defect in EBV-immune surveillance in patients receiving MTX or anti-TNF drugs. However, in these patients, long term follow-up of EBV-specific T-cell response is necessary and the role of non-EBV-related mechanisms of lymphomagenesis is not excluded

Enhancement of pure titanium localized corrosion resistance by anodic oxidation

The corrosion behavior of commercially pure titanium (UNS R50400) was investigated in presence of aggressive, bromides containing, species; reported to cause severer localized corrosion compared to chlorides. To enhance localized corrosion resistance of the metal, several surface treatments were performed. Samples anodized at potentials between 10 V and 200 V were characterized in term of oxide thickness and morphology and tested with potentiodynamic analyses in NH4Br. This treatment was found to greatly enhance corrosion resistance of titanium but it suffers localized removal of the oxide due to wrong handling of the part before their installation. For this reason, another treatment, suitable for in-situ surface recovering was developed through chemical oxidation in NaOH 10 M

GROWTH AND YIELD TRAITS OF GROUNDNUT(ARACHIS HYPOGAEA) LINES TREATED WITH HYDROGEN PEROXIDE

The response of some growth and yield traits of four  groundnut cultivars, Ms 54-76, ICGV-Sm-42, RmP 12 and Samnut 21 treated with hydrogen peroxide for 24 hours prior to planting was studied using seed treatmentof 0%, 0.5%, 1%, 2% and 3%of hydrogen peroxide (H2O2). The treated seeds were planted in a split plot experimental designing a completely randomised design (CRD) using three replications in University of Calabar, Nigeria. Results showed that more seedlings of Ms 54-76, ICGV-Sm-42 and RmP 12 were established than Samnut 21 at fourteen days after planting. There was a similar variation in the number of days to 50% flowering, number of pods per plant, plant height, number of leaves, number of branches, and weight of pods per plant, shelling percentage and seed weight. The H2O2 incited increase in the seed germination, plant height and in seeds weight from 8 weeks after planting upwards. Generally, ICGV-Sm-42 had the most luxuriant growth and superior yield of the four varieties, followed by Sm-54-76 and RmP 12.ICGV-Sm-42 had the highest response to seed treatment with hydrogen peroxid

Performance da Prótese adesiva anterior Uma revisão sistemática integrativa

No edentulismo anterior, várias opções são possíveis, incluindo próteses adesivas. Seus avanços permitiram que fossem cada vez mais consideradas como uma terapia promissora a longo prazo. O objetivo principal do trabalho foi realizar uma revisão sistemática integrativa sobre a Perfomance da prótese adesiva anterior. Foi realizada uma pesquisa eletrónica na base de dados PubMed, utilizando um limite temporal de 10 anos (2013-2023), usando os MeshTerms and Supplementary Concepts: “Anterior Cantilever resin-bonded bridge”, “Anterior single-retainer resin-bonded fixed prostheses”, “Prostheses fixed partial”, “Dental bonding”, “Dental abutments”. Utilizando diferentes combinações destes MeshTerms and Supplementary Concepts sob forma de Expressões de pesquisa, foram encontrados 66 resultados (depois da remoção dos duplicados) e segundo critérios de inclusão e exclusão foram selecionados 22 artigos para a redação do trabalho. Várias opções de tratamento são possíveis na ausência de dente(s) anterior(es), incluindo a prótese adesiva anterior fixa. A sua conceção pode ser conseguida através de diversos materiais, podendo ser concebida em diferentes formas. Os avanços tecnológicos permitem a sua constante melhoria e evolução. A prótese adesiva anterior é uma opção totalmente viável para o paciente, mesmo a médio e longo prazo, e oferece muitas vantagens em relação a outros possíveis tratamentos existentes.With an anterior tooth loss, several options are possible, including adhesive prostheses. Their advances have allowed them to be increasingly seen as a promising long-term therapy. The main objective of the work was to carry out an integrative systematic review about the Performance of anterior adhesive prosthese. An electronic search was performed in the PubMed database, using a time limit of 10 years (2013-2023), using the MeshTerms and Supplementary Concepts: “Anterior Cantilever resin-bonded bridge”, “Anterior single-retainer resin-bonded fixed prostheses ”, “Prostheses fixed partial”, “Dental bonding”, “Dental abutments”. Using different combinations of these MeshTerms and Supplementary Concepts in the form of Search Expressions, 66 results were found (after removing duplicates) and according to inclusion and exclusion criteria 22 articles were selected for the writing of the work. Several treatment options are possible in the absence of anterior tooth(s), including the fixed anterior adhesive prosthesis. Its design can be achieved through several materials, and can be designed in different forms. Technological advances allow its constant improvement and evolution. The anterior adhesive prosthesis is a completely viable option for the patient, even in the medium to long term, and offers many advantages over other possible existing treatments

Response of cowpea lines to inoculation with four seed transmitted viruses of cowpea.

This study evaluated the performance cowpea genotypes in the field in Ibadan, Nigeria for response to infection induced by four seed transmitted viruses of cowpea. The experiment was laid out in split plot design withthree replicates. Cowpea mottle virus genus Carmovirus (CMeV) produced infection in 14 of the 15 lines, Bean common mosaic virus genus Potyvirus - blackeye cowpea strain (BCMV – BlC) in 12, Cowpea aphid-borne mosaicvirus genus Potyvirus (CABMV) in 11 and Southern bean mosaic virus genus Sobemovirus (SBMV) in 6. BCMV – BlC significantly reduced (

Conservation of a pH-sensitive structure in the C-terminal region of spider silk extends across the entire silk gene family

Spiders produce multiple silks with different physical properties that allow them to occupy a diverse range of ecological niches, including the underwater environment. Despite this functional diversity, past molecular analyses show a high degree of amino acid sequence similarity between C-terminal regions of silk genes that appear to be independent of the physical properties of the resulting silks; instead, this domain is crucial to the formation of silk fibres. Here we present an analysis of the C-terminal domain of all known types of spider silk and include silk sequences from the spider Argyroneta aquatica, which spins the majority of its silk underwater. Our work indicates that spiders have retained a highly conserved mechanism of silk assembly, despite the extraordinary diversification of species, silk types and applications of silk over 350 million years. Sequence analysis of the silk C-terminal domain across the entire gene family shows the conservation of two uncommon amino acids that are implicated in the formation of a salt bridge, a functional bond essential to protein assembly. This conservation extends to the novel sequences isolated from A. aquatica. This finding is relevant to research regarding the artificial synthesis of spider silk, suggesting that synthesis of all silk types will be possible using a single process

Revisiting the B-cell compartment in mouse and humans: more than one B-cell subset exists in the marginal zone and beyond.

International audienceABSTRACT: The immunological roles of B-cells are being revealed as increasingly complex by functions that are largely beyond their commitment to differentiate into plasma cells and produce antibodies, the key molecular protagonists of innate immunity, and also by their compartmentalisation, a more recently acknowledged property of this immune cell category. For decades, B-cells have been recognised by their expression of an immunoglobulin that serves the function of an antigen receptor, which mediates intracellular signalling assisted by companion molecules. As such, B-cells were considered simple in their functioning compared to the other major type of immune cell, the T-lymphocytes, which comprise conventional T-lymphocyte subsets with seminal roles in homeostasis and pathology, and non-conventional T-lymphocyte subsets for which increasing knowledge is accumulating. Since the discovery that the B-cell family included two distinct categories - the non-conventional, or extrafollicular, B1 cells, that have mainly been characterised in the mouse; and the conventional, or lymph node type, B2 cells - plus the detailed description of the main B-cell regulator, FcγRIIb, and the function of CD40+ antigen presenting cells as committed/memory B-cells, progress in B-cell physiology has been slower than in other areas of immunology. Cellular and molecular tools have enabled the revival of innate immunity by allowing almost all aspects of cellular immunology to be re-visited. As such, B-cells were found to express "Pathogen Recognition Receptors" such as TLRs, and use them in concert with B-cell signalling during innate and adaptive immunity. An era of B-cell phenotypic and functional analysis thus began that encompassed the study of B-cell microanatomy principally in the lymph nodes, spleen and mucosae. The novel discovery of the differential localisation of B-cells with distinct phenotypes and functions revealed the compartmentalisation of B-cells. This review thus aims to describe novel findings regarding the B-cell compartments found in the mouse as a model organism, and in human physiology and pathology. It must be emphasised that some differences are noticeable between the mouse and human systems, thus increasing the complexity of B-cell compartmentalisation. Special attention will be given to the (lymph node and spleen) marginal zones, which represent major crossroads for B-cell types and functions and a challenge for understanding better the role of B-cell specificities in innate and adaptive immunology

Variations in agromorphological characteristics of IPGRI Cowpea (Vigna unguiculata (L.) Walp.) accessions

Cowpea (Vigna unguiculata (L.) Walp.) remains an important crop in the tropics. One of its challenges remains the selection and improvement of genotypes to meet location–specific needs. We studied ten cowpea accessions in Akamkpa and Ikom, Cross River State, Nigeria. Principal component and biplot analyses associated high yield with growth, flowering, pod and seed traits. Seed weight per plot and number of seeds per plot were phenotypically correlated (rp = 0.99, p ≤ 0.01). Number of seeds per plot and number of seeds per pod were genotypically correlated (rg = 1.00, p ≤ 0.01). TVu–980 did not flower at Akamkpa and TVu–1019 did not grow at Ikom. TVu–992, TVu–53, TVu11, TVu–3629 and TVu–980 (only at Ikom) cowpea accessions showed promising agromorphological attributes for possible crop improvement programmes in Ikom and Akamkpa

B cell-activating factor of the tumor necrosis factor family (BAFF) is expressed under stimulation by interferon in salivary gland epithelial cells in primary Sjögren's syndrome

B cell-activating factor (BAFF) has a key role in promoting B-lymphocyte activation and survival in primary Sjögren's syndrome (pSS). The cellular origin of BAFF overexpression in salivary glands of patients with pSS is not fully known. We investigated whether salivary gland epithelial cells (SGECs), the main targets of autoimmunity in pSS, could produce and express BAFF. We used quantitative RT-PCR, ELISA and immunocytochemistry in cultured SGECs from eight patients with pSS and eight controls on treatment with IL-10, tumor necrosis factor α (TNF-α), IFN-α and IFN-γ. At baseline, BAFF expression in SGECs was low in pSS patients and in controls. Treatment with IFN-α, IFN-γ and TNF-α + IFN-γ increased the level of BAFF mRNA in pSS patients (the mean increases were 27-fold, 25-fold and 62-fold, respectively) and in controls (mean increases 19.1-fold, 26.7-fold and 17.7-fold, respectively), with no significant difference between patients and controls. However, in comparison with that at baseline, stimulation with IFN-α significantly increased the level of BAFF mRNA in SGECs of pSS patients (p = 0.03) but not in controls (p = 0.2), which suggests that SGECs of patients with pSS are particularly susceptible to expressing BAFF under IFN-α stimulation. Secretion of BAFF protein, undetectable at baseline, was significantly increased after IFN-α and IFN-γ stimulation both in pSS patients (40.8 ± 12.5 (± SEM) and 47.4 ± 18.7 pg/ml, respectively) and controls (24.9 ± 8.0 and 9.0 ± 3.9 pg/ml, respectively), with no significant difference between pSS and controls. Immunocytochemistry confirmed the induction of cytoplasmic BAFF expression after stimulation with IFN-α and IFN-γ. This study confirms the importance of resident cells of target organs in inducing or perpetuating autoimmunity. Demonstrating the capacity of SGECs to express and secrete BAFF after IFN stimulation adds further information to the pivotal role of these epithelial cells in the pathogenesis of pSS, possibly after stimulation by innate immunity. Our results suggest that an anti-BAFF therapeutic approach could be particularly interesting in pSS