A single supplement of a standardised bilberry (Vaccinium myrtillus L.) extract (36 % wet weight anthocyanins) modifies glycaemic response in individuals with type 2 diabetes controlled by diet and lifestyle

Peer reviewedPublisher PD

Development of Bacillus thuringiensis CryIC resistance by Spodoptera exigua (Huebner) (Lepidoptera : Noctuidae)

Selection of resistance in Spodoptera exigua (Hubner) to an HD-1 spore-crystal mixture, CryIC (HD-133) inclusion bodies, and trypsinized toxin from Bacillus thuringiensis' subsp, aizawai and B. thuringiensis subsp. entomocidus was attempted by using laboratory bioassays. No resistance to the HD-1 spore-crystal mixture could be achieved after 20 generations of selection. Significant levels of resistance (11-fold) to CryIC inclusion bodies expressed in Escherichia coli were observed after seven generations, Subsequent selection of the CryIC-resistant population with trypsinized CryIC toxin resulted, after 21 generations of CryIC selection, in a population of S. exigua that exhibited only 8% mortality at the highest toxin concentration tested (320 mu g/g), whereas the 50% lethal concentration was 4.30 mu g/g for the susceptible colony. Insects resistant to CryIC toxin from HD-133 also were resistant to trypsinized CryIA(b), CryIC from B. thuringiensis subsp. entomocidus, CryIE-CryIC fusion protein (G27), CryIH, and CryIIA. In vitro binding experiments with brush border membrane vesicles showed a twofold decrease in maximum CryIC binding, a fivefold difference in K-d, and no difference in the concentration of binding sites for the CryIC-resistant insects compared with those for the susceptible insects, Resistance to CryIC was significantly reduced by the addition of HD-1 spores, Resistance to the CryIC toxin was still observed 12 generations after CryIC selection was removed. These results suggest that, in S. exigua, resistance to a single protein is more likely to occur than resistance to spore crystal mixtures and that once resistance occurs, insects will be resistant to many other Cry proteins, These results have important implications for devising S. exigua resistance management strategies in the field

Local multiplayer immersion affected by 3D stereoscopy

In this paper, we describe an experimental study, which evaluates how 3D stereoscopy affects player immersion in a possibly very distracting local multiplayer game. The game “Nicely Dicely” was specifically developed for this purpose, with 3D stereoscopy in mind, right from the beginning. Groups of participants were competitively playing the game in non-3D monoscopic and 3D stereoscopic presentations via a 3D compatible projector and corresponding active shutter glasses. In the following, we elaborate on the game and our quantitative and qualitative hybrid experiment design and methodology. An analysis of the resulting data will show that, indeed 3D stereoscopy significantly increases spatial presence, involvement and player immersion, even in a local multiplayer situation. Furthermore, some guiding insights relating the game’s design will be illustrated

Toxicity of Bacillus thuringiensis CrylAb Toxin to the Predator Chrysoperla carnea (Neuroptera: Chrysopidae)

Laboratory feeding studies were carried out to determine the effects of the Bacillus thuringiensis (Berliner) CrylAb toxin on developmental time and mortality of Chrysoperla carnea (Stephens) larvae. A bioassay technique was developed that allowed for incorporation of the CrylAb toxin into a liquid diet that was then encapsulated within small paraffin spheres. Because only 2nd and 3rd instars can penetrate the surface of the paraffin spheres, 2 different methods were used to rear chrysopid larvae through the 1st instar. The 1st method used small foam cubes soaked in non-encapsulated, liquid diet (with or without CrylAb). The 2nd method used Ephestia kuehniella (Hübner) eggs as prey during the first instar (no CrylAb exposure). After reaching the 2nd instar, all larvae received encapsulated, artificial diet with or without CrylAb, respectively. When reared only on artificial diet containing CrylAb toxin, total immature mortality was significantly higher (57%) than in the respective untreated control (30%). Also, significantly more chrysopid larvae died (29%) that received CrylAb later during their larval development compared with the respective control (17%). Although mortality was consistently higher, no or only small differences in developmental times were observed between CrylAb-treated and untreated C. carnea larvae. C. carnea larvae required significantly more time to complete larval development when reared on artificial diet only than when reared first on E. kuehniella eggs followed by encapsulated artificial diet or on only E. kuehniella eggs, regardless of exposure to CrylAb. These results demonstrate that CrylAb is toxic to C. carnea at 100 μmg/ml of diet by using encapsulated artificial die

Node-by-node disassembly of a mutualistic interaction web driven by species introductions

Interaction webs summarize the diverse interactions among species in communities. The addition or loss of particular species and the alteration of key interactions can lead to the disassembly of the entire interaction web, although the nontrophic effects of species loss on interaction webs are poorly understood. We took advantage of ongoing invasions by a suite of exotic species to examine their impact in terms of the disassembly of an interaction web in Patagonia, Argentina. We found that the reduction of one species (a host of a keystone mistletoe species) resulted in diverse indirect effects that led to the disassembly of an interaction web through the loss of the mistletoe, two key seed-dispersers (a marsupial and a bird), and a pollinator (hummingbird). Our results demonstrate that the gains and losses of species are both consequences and drivers of global change that can lead to underappreciated cascading coextinctions through the disruption of mutualisms.Fil: Rodriguez Cabal, Mariano Alberto. University Of Tennessee; Estados Unidos. University Of British Columbia; CanadáFil: Barrios Garcia Moar, Maria Noelia. University Of Tennessee; Estados Unidos. University Of British Columbia; CanadáFil: Amico, Guillermo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; ArgentinaFil: Aizen, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; ArgentinaFil: Sanders, Nathan J.. University Of Tennessee; Estados Unidos. Natural History Museun Of Copenhague; Dinamarc

VR and the dramatic theatre: are they fellow creatures?

This paper describes an experimental project that aims to investigate the scope of methodological and technical possibilities of using 360° videos for experiencing authored drama. In particular, it examines how a work written for the medium of theatre, with a traditional audience-drama relationship of viewer invisibility and non-participation, might translate into a viewing experience as a VR drama. The technical and dramaturgical issues arising from this are discussed. Specifically, the shared voyeuristic quality of both media is examined. Is the invisible viewer of VR drama in the invidious role of Glaucon’s iniquitous shepherd Gyges, or does the medium give invisibility a cloak of aesthetic value

A desire for immersion: the panorama to the Oculus Rift

The paper argues that the experience of viewing 360° historical scenes, either within the "real" environment of the panorama or contemporary "virtual" environments, arises in part from the attraction of the affecting experience. Tracing a line from ancient Greece and Rome through to contemporary technological innovations in Virtual Reality, we explore an idea that what links all of these experiences is not solely a response to social, political or historical streams but a manifestation of a pan-historical human desire for the Dionysiac. Using texts by theorists as diverse as Nietzsche, Baudrillard, Rheingold, Gibson and Shaviro, we suggest that the impetus for viewing historical scenes within 360° environments is at least partly involved in wanting to go beyond spectatorship and intellectual curiousity, towards presence and intoxication. Attempts to tease out the ideological content of an immersive experience must be done in the context of the craving of audience members for the sensual, the sensational, the being present, and other "illegitimate" forms of impetus to view and experiences. These always already exist alongside and perhaps even before the more legitimate goals of gaining knowledge, historical perspective, education and information. Charlton and Moar explore the idea that this desire for immersion impels us to submerge ourselves in historical scenes. Thus immersion is the link between panoramas and new panoramic experiences afforded by new technologies such as the Oculus Rift