Transport of Babesia venatorum-infected Ixodes ricinus to Norway by northward migrating passerine birds

<p>Abstract</p> <p>Background</p> <p>Bovine babesiosis is regarded as a limited health problem for Norwegian cows, and the incidence has decreased markedly since the 1930s. Rare cases of babesiosis in splenectomised humans from infection with <it>Babesia divergens </it>and <it>B.venatorum </it>have been described. The objective of this study was to determine whether birds can introduce <it>Babesia</it>-infected ticks. There are between 30 and 85 million passerine birds that migrate to Norway every spring.</p> <p>Methods</p> <p>Passerine birds were examined for ticks at four bird observatories along the southern Norwegian coast during the spring migrations of 2003, 2004 and 2005. The presence of <it>Babesia </it>was detected in the nymphs of <it>Ixodes ricinus </it>by real-time PCR. Positive samples were confirmed using PCR, cloning and phylogenetic analyses.</p> <p>Results</p> <p>Of 512 ticks examined, real-time PCR revealed five to be positive (1.0%). Of these, four generated products that indicated the presence of <it>Babesia </it>spp.; each of these were confirmed to be from <it>Babesia venatorum </it>(EU1). Two of the four <it>B. venatorum</it>-positive ticks were caught from birds having an eastern migratory route (<it>P</it>< 0.001).</p> <p>Conclusions</p> <p>Birds transport millions of ticks across the North Sea, the Skagerrak and the Kattegat every year. Thus, even with the low prevalence of <it>Babesia</it>-infected ticks, a substantial number of infected ticks will be transported into Norway each year. Therefore, there is a continuous risk for introduction of new <it>Babesia </it>spp. into areas where <it>I. ricinus </it>can survive.</p

Detection of Babesia divergens in southern Norway by using an immunofluorescence antibody test in cow sera

<p>Abstract</p> <p>Background</p> <p>The incidence of bovine babesiosis, caused by <it>Babesia divergens </it>(Apicomplexa: Piroplasmida) has decreased markedly since the 1930 s, but may re-emerge as a consequence of climate change and changes in legislation and pasturing practices. This is a potentially serious disease, with both economical and animal welfare consequences. Therefore, there is a need to survey the distribution of <it>B. divergens</it>.</p> <p>Methods</p> <p>We tested sera from 306 healthy pastured cows from 24 farms along the southern Norwegian coast by using an indirect immunofluorescence IgG antibody test (IFAT). Fractions of seropositive cows were compared by calculating 95% CI.</p> <p>Results</p> <p>The results of this test showed that 27% of the sera were positive for <it>B. divergens </it>antibodies. The fraction of antibody-positive sera that we detected showed a two-humped distribution, with a high fraction of positives being found in municipalities in the western and eastern parts of the study area, while the municipalities between these areas had few or no positive serum samples.</p> <p>Conclusions</p> <p>Neither the farmers' observations nor the Norwegian Dairy Herd Recording System give an adequate picture of the distribution of bovine babesiosis. Serological testing of cows by using IFAT is a convenient way of screening for the presence of <it>B. divergens </it>in an area.</p

Dermacentor reticulatus: a vector on the rise

Dermacentor reticulatus is a hard tick species with extraordinary biological features. It has a high reproduction rate, a rapid developmental cycle, and is also able to overcome years of unfavourable conditions. Dermacentor reticulatus can survive under water for several months and is cold-hardy even compared to other tick species. It has a wide host range: over 60 different wild and domesticated hosts are known for the three active developmental stages. Its high adaptiveness gives an edge to this tick species as shown by new data on the emergence and establishment of D. reticulatus populations throughout Europe. The tick has been the research focus of a growing number of scientists, physicians and veterinarians. Within the Web of Science database, more than a fifth of the over 700 items published on this species between 1897 and 2015 appeared in the last three years (2013–2015). Here we attempt to synthesize current knowledge on the systematics, ecology, geographical distribution and recent spread of the species and to highlight the great spectrum of possible veterinary and public health threats it poses. Canine babesiosis caused by Babesia canis is a severe leading canine vector-borne disease in many endemic areas. Although less frequently than Ixodes ricinus, D. reticulatus adults bite humans and transmit several Rickettsia spp., Omsk haemorrhagic fever virus or Tick-borne encephalitis virus. We have not solely collected and reviewed the latest and fundamental scientific papers available in primary databases but also widened our scope to books, theses, conference papers and specialists colleagues’ experience where needed. Besides the dominant literature available in English, we also tried to access scientific literature in German, Russian and eastern European languages as well. We hope to inspire future research projects that are necessary to understand the basic life-cycle and ecology of this vector in order to understand and prevent disease threats. We conclude that although great strides have been made in our knowledge of the eco-epidemiology of this species, several gaps still need to be filled with basic research, targeting possible reservoir and vector roles and the key factors resulting in the observed geographical spread of D. reticulatus. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1599-x) contains supplementary material, which is available to authorized users

Rodents as carriers of tick-borne zoonotic diseases and their ecological impact

Rodents as pests are known to be reservoirs of tick-borne zoonotic infection of viral, bacterial and protozoan origin and are important hosts of the immature stages of Ixodes ticks. A total 493 rodents were live-trapped in different habitat in two biogeographical regions Lithuania and Norway. The ecological impact of different species of rodents in particular habitats was evaluated according to their infestation with ticks and presence of Borrelia burgdorferi s.l. and Anaplasma phagocytophilum infection. Results of the present study demonstrate that although the infestation rate varied between rodent species it was dependent on sampling location and not correlated with infection prevalence in rodents. We observed that mice in Lithuania were more frequently infested with ticks than voles, but, the higher prevalence of B. burgdorferi s.l. infection was detected in voles, than in mice. The overall prevalence of infection in Lithuania was higher than in Norway. In Norway, prevalence of infection in A. flavicollis and M. glareolus was not significantly different. The present study confirmed evidence that the rodent impact on maintenance of Lyme borreliosis depends on biogeographical region and habitat type, and that the zoonotic reservoirs of B. afzelii are M. arvalis, M. glareolus, and A. flavicollis in Lithuania, and A. flavicollis, A. sylvaticus and M. glareolus in Norway. In our present study A. phagocytophilum was not detected in any of the rodents

First report of heartworm (Dirofilaria immitis) infection in an imported dog in Lithuania

Over the past decade, increasing numbers of autochthonous cases of heartworm infection have been reported in the countries of Eastern Europe where previously only imported cases were described. In this report we have described the first clinical case of Dirofilaria immitis infection in an imported dog in Lithuania

First detection of Rickettsia helvetica in small mammals in Lithuania

A total of 489 small mammals belonging to seven species captured in Lithuania during 2013–2014 were investigated for Rickettsia pathogens. The overall prevalence of Rickettsia spp. was 27.6%, with a higher prevalence detected in Micromys minutus (45.9%), followed by Apodemus flavicollis (29.4%), Sorex araneus (25%) and Myodes glareolus (23.7%). Sequence analysis of the gltA gene and the 17 kDa protein coding gene revealed the presence Rickettsia helvetica. This study demonstrates not only the first reported presence of R. helvetica in small mammals in Lithuania but also the first report of R. helvetica in M. minutus more generally. Keywords: 17 kDa, gltA, Lithuania, Rickettsia helvetica, rodents, shrew