Searching for tidal tails - investigating galaxy harassment

Galaxy harassment has been proposed as a physical process that morphologically transforms low surface density disc galaxies into dwarf elliptical galaxies in clusters. It has been used to link the observed very different morphology of distant cluster galaxies (relatively more blue galaxies with 'disturbed' morphologies) with the relatively large numbers of dwarf elliptical galaxies found in nearby clusters. One prediction of the harassment model is that the remnant galaxies should lie on low surface brightness tidal streams or arcs. We demonstrate in this paper that we have an analysis method that is sensitive to the detection of arcs down to a surface brightness of 29 B mag/arcsec^2 and then use this method to search for arcs around 46 Virgo cluster dwarf elliptical galaxies. We find no evidence for tidal streams or arcs and consequently no evidence for galaxy harassment as a viable explanation for the relatively large numbers of dwarf galaxies found in the Virgo cluster.Comment: Accepted for publication in MNRA

Orbital and meteorological factors pertinent to satellite transmissions of facsimile weather charts Final report

Automatic picture transmission system on Nimbus and earth synchronous satellites for transmission of weather chart

The dwarf low surface brightness population in different environments of the Local Universe

The nature of the dwarf galaxy population as a function of location in the cluster and within different environments is investigated. We have previously described the results of a search for low surface brightness objects in data drawn from an East-West strip of the Virgo cluster (Sabatini et al., 2003) and have compared this to a large area strip outside of the cluster (Roberts et al., 2004). In this talk I compare the East-West data (sampling sub-cluster A and outward) to new data along a North-South cluster strip that samples a different region (part of sub-cluster A, and the N,M clouds) and with data obtained for the Ursa Major cluster and fields around the spiral galaxy M101. The sample of dwarf galaxies in different environments is obtained from uniform datasets that reach central surface brightness values of ~26 B mag/arcsec^2 and an apparent B magnitude of 21 (M_B=-10 for a Virgo Cluster distance of 16 Mpc). We discuss and interpret our results on the properties and distribution of dwarf low surface brightness galaxies in the context of variuos physical processes that are thought to act on galaxies as they form and evolve.Comment: 10 pages, 3 figures, to appear in "Dark Galaxies and Lost Baryons", IAU244 conference proceeding

Management, processing and dissemination of sensory data for the Earth Resource Technology Satellite

Data center for management, processing, and dissemination of photographic products generated by ERT

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 system for genome editing has greatly expanded the toolbox for mammalian genetics, enabling the rapid generation of isogenic cell lines and mice with modified alleles. Here, we describe a pooled, loss-of-function genetic screening approach suitable for both positive and negative selection that uses a genome-scale lentiviral single-guide RNA (sgRNA) library. sgRNA expression cassettes were stably integrated into the genome, which enabled a complex mutant pool to be tracked by massively parallel sequencing. We used a library containing 73,000 sgRNAs to generate knockout collections and performed screens in two human cell lines. A screen for resistance to the nucleotide analog 6-thioguanine identified all expected members of the DNA mismatch repair pathway, whereas another for the DNA topoisomerase II (TOP2A) poison etoposide identified TOP2A, as expected, and also cyclin-dependent kinase 6, CDK6. A negative selection screen for essential genes identified numerous gene sets corresponding to fundamental processes. Last, we show that sgRNA efficiency is associated with specific sequence motifs, enabling the prediction of more effective sgRNAs. Collectively, these results establish Cas9/sgRNA screens as a powerful tool for systematic genetic analysis in mammalian cells.National Institutes of Health (U.S.) (CA103866)National Human Genome Research Institute (U.S.) (2U54HG003067-10)National Science Foundation (U.S.

CRISPR/Cas9-Mediated Gene Knock-Down in Post-Mitotic Neurons

The prokaryotic adaptive immune system CRISPR/Cas9 has recently been adapted for genome editing in eukaryotic cells. This technique allows for sequence-specific induction of double-strand breaks in genomic DNA of individual cells, effectively resulting in knock-out of targeted genes. It thus promises to be an ideal candidate for application in neuroscience where constitutive genetic modifications are frequently either lethal or ineffective due to adaptive changes of the brain. Here we use CRISPR/Cas9 to knock-out Grin1, the gene encoding the obligatory NMDA receptor subunit protein GluN1, in a sparse population of mouse pyramidal neurons. Within this genetically mosaic tissue, manipulated cells lack synaptic current mediated by NMDA-type glutamate receptors consistent with complete knock-out of the targeted gene. Our results show the first proof-of-principle demonstration of CRISPR/Cas9-mediated knock-down in neurons in vivo, where it can be a useful tool to study the function of specific proteins in neuronal circuits

Características del zooplancton del área costera de la plataforma patagónica austral (Argentina)

El estudio del zooplancton en áreas de desove y cría de peces es de particular trascendencia en la región costera patagónica sur (50º-55º S), dado que allí se distribuye la población continental de sardina fueguina Sprattus fuegensis (Jenyns, 1842); esta especie es exclusivamente zooplanctófaga a lo largo de todo su ciclo vital y se le atribuye el papel de especie forrajera en el sector austral del mar Argentino. En el otoño y la primavera de 1996 se colectaron muestras de zooplancton en el área mediante arrastres oblicuos fondo-superficie con un muestreador Nackthai provisto de una red de 400 μm de luz de malla y flujómetro digital. El material se separó en dos grupos según el tamaño (mayor y menor de 5 mm) y se pesaron las fracciones resultantes (peso húmedo). En ambas estaciones del año, la biomasa de la fracción menor fue normalmente igual, e incluso superó en ocasiones, a la concentración de la fracción mayor. Dada su importancia para los objetivos de este trabajo, se analizó en detalle la composición y abundancia numérica de la fracción menor de 5 mm, que se mostró compuesta casi exclusivamente por copépodos calanoideos, dominados por las especies Drepanopus forcipatus Giesbrecht, 1888 y Calanus australis Brodsky, 1959. La biomasa, la abundancia de individuos y el número de especies fueron mayores en primavera. En esta estación Drepanopus forcipatus fue la especie dominante en casi toda el área, constituyendo hasta el 90 % del total de individuos. Calanus australis fue relativamente más importante en otoño, periodo en que dominó, en particular, en la zona de Bahía Grande. Estos dos copépodos pueden considerarse especies clave en la estructuración de las tramas tróficas del área.Fil: Sabatini, Marina Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Subsede Instituto Nacional de Investigación y Desarrollo Pesquero; ArgentinaFil: Giménez, J.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaFil: Rocco, V.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentin

Low-cost sensors based multi-sensor data fusion techniques for RPAS navigation and guidance

In order for Remotely Piloted Aircraft Systems (RPAS) to coexist seamlessly with manned aircraft in non-segregated airspace, enhanced navigational capabilities are essential to meet the Required Navigational Performance (RNP) levels in all flight phases. A Multi-Sensor Data Fusion (MSDF) framework is adopted to improve the navigation capabilities of an integrated Navigation and Guidance System (NGS) designed for small-sized RPAS. The MSDF architecture includes low-cost and low weight/volume navigation sensors suitable for various classes of RPAS. The selected sensors include Global Navigation Satellite Systems (GNSS), Micro-Electro-Mechanical System (MEMS) based Inertial Measurement Unit (IMU) and Vision Based Sensors (VBS). A loosely integrated navigation architecture is presented where an Unscented Kalman Filter (UKF) is used to combine the navigation sensor measurements. The presented UKF based VBS-INS-GNSS-ADM (U-VIGA) architecture is an evolution of previous research performed on Extended Kalman Filter (EKF) based VBS-INS-GNSS (E-VIGA) systems. An Aircraft Dynamics Model (ADM) is adopted as a virtual sensor and acts as a knowledge-based module providing additional position and attitude information, which is pre-processed by an additional/local UKF. The E-VIGA and U-VIGA performances are evaluated in a small RPAS integration scheme (i.e., AEROSONDE RPAS platform) by exploring a representative cross-section of this RPAS operational flight envelope. The position and attitude accuracy comparison shows that the E-VIGA and U-VIGA systems fulfill the relevant RNP criteria, including precision approach in CAT-II. A novel Human Machine Interface (HMI) architecture is also presented, whose design takes into consideration the coordination tasks of multiple human operators. In addition, the interface scheme incorporates the human operator as an integral part of the control loop providing a higher level of situational awareness

A laser obstacle detection and avoidance system for manned and unmanned aircraft applications

This paper presents the key design features, the numerical simulations and the experimental ground/flight test activities performed to verify the functionalities of an obstacle detection and avoidance system suitable for various classes of manned and unmanned aircraft. The Laser Obstacle Avoidance and Monitoring (LOAM) system is proposed as one of the key non-cooperative sensors adopted for avoiding obstacles/intruders in the context of a future Sense-and-Avoid (SAA) capability. After a brief description of the system architecture and of the main data processing algorithms, avoidance trajectory generation and performance estimation models are described. A simulation of the avoidance trajectory generation algorithm is performed in a realistic scenario. Additionally, a brief overview of ground and flight test activities performed on various platforms and their main results is also presented. Some of the key aspects of the LOAM Human Machine Interface and Interaction (HMI2) design are also outlined. The demonstrated detection and avoidance performances and the robust trajectory generation algorithm ensure a safe avoidance of all classes of obstacles (i.e. ground and aerial) in all weather conditions and flight phases

A multi-beam HI survey of the Virgo Cluster - two isolated HI clouds ?

We have carried out a fully sampled large area ($4^{\circ} \times 8^{\circ}$) 21cm \HI line survey of part of the Virgo cluster using the Jodrell Bank multi-beam instrument. The survey has a sensitivity some 3 times better than the standard HIJASS and HIPASS surveys. We detect 31 galaxies, 27 of which are well known cluster members. The four new detections have been confirmed in the HIPASS data and by follow up Jodrell Bank pointed observations. One object lies behind M86, but the other 3 have no obvious optical counter parts upon inspection of the digital sky survey fields. These 3 objects were mapped at Arecibo with a smaller \am{3}{6} HPBW and a 4 times better sensitivity than the Jodrell Bank data, which allow an improved determination of the dimensions and location of two of the objects, but surprisingly failed to detect the third. The two objects are resolved by the Arecibo beam giving them a size far larger than any optical images in the nearby field. To our mass limit of $5 \times 10^{7}$ $\frac{\Delta v}{50 km s^{-1}}$ $M_{\odot}$ and column density limit of $3 \times 10^{18}$ $\frac{\Delta v}{50 km s^{-1}}$ atoms cm$^{-2}$ these new detections represent only about 2% of the cluster atomic hydrogen mass. Our observations indicate that the \HI mass function of the cluster turns down at the low mass end making it very different to the field galaxy \HI mass function. This is quite different to the Virgo cluster optical luminosity function which is much steeper than that in the general field. Many of the sample galaxies are relatively gas poor compared to \HI selected samples of field galaxies, confirming the 'anaemic spirals' view of Virgo cluster late type galaxies.Comment: Accepted for publication in MNRA