Cytokine receptor expression in human lymphoid tissue: analysis by fluorescence microscopy

A highly-sensitive flourescence method, capable of detecting cytokine receptors present at low concentrations (around I DO molecules per cell) by flow cytometry, was adapted for use on tissue sections. This method was used to examine the expression of several cytokine receptors in lymphoid ti ss ues. lL-2 receptors were distributed broadly, with higher concentrations in T cell areas. lL-1 receptor Type I was detected in T cell areas and in the follicular mantle, and was strongly expressed on vasc ular endothelium. IL-6 receptor was found at very low concentration, both within and outside germinal centres. The gp 130 molecule, which is involved in the functional receptor complex for IL-6 and several other cytokines, was present at higher concentrations, particularly in the germinal centre. Analysis of receptor expression in secondary lymphoid tissue provides evidence bearing on the physiological roles of cytokines, as these tissues contain cells at various stages of physiological activation located in well-defined functional zones.Heddy Zola, Jodie Ridings, Helen Weedon, Michael Fusco, Roger W. Byard, Peter J. Macardl

Leistung und agronomisches Potenzial von Composite Cross Winterweizen-Populationen und anderen Phänotypen in einem Mischanbausystem

Unpredictable climatic conditions and increasing pressure on non-renewable resources mean that alternative agricultural systems able to cope with increasing biotic and abiotic stresses, as well as external input costs, are needed. Sustainable agricultural systems should be self-regulating, characterized by a high degree of interand intra-specific diversity (Moreau 2010). Evolutionary breeding through the introduction of Composite Cross Populations (CCPs) increases intra-specific diversity, enabling CCPs to adapt to changing environmental conditions. Increasing interspecific diversity, through mixed cropping, also plays an important role in sustainable systems and suitable crops are needed that are able to cope with the increasing interspecific competition found in these mixed systems. A number of winter wheat CCPs, lines and commercial varieties were tested with white clover as an undersowing in order to test competitive ability and performance

Modern mammography screening and breast cancer mortality: population study

Objective: To evaluate the effectiveness of contemporary mammography screening using individual information about screening history and breast cancer mortality from public screening programmes. Design: Prospective cohort study of Norwegian women who were followed between 1986 and 2009. Within that period (1995-2005), a national mammography screening programme was gradually implemented, with biennial invitations sent to women aged 50-69 years. Participants: All Norwegian women aged 50-79 between 1986 and 2009. Main outcome measures Multiple Poisson regression analysis was used to estimate breast cancer mortality rate ratios comparing women who were invited to screening (intention to screen) with women who were not invited, with a clear distinction between cases of breast cancer diagnosed before (without potential for screening effect) and after (with potential for screening effect) the first invitation for screening. We took competing causes of death into account by censoring women from further follow-up who died from other causes. Based on the observed mortality reduction combined with the all cause and breast cancer specific mortality in Norway in 2009, we used the CISNET (Cancer Intervention and Surveillance Modeling Network) Stanford simulation model to estimate how many women would need to be invited to biennial mammography screening in the age group 50-69 years to prevent one breast cancer death during their lifetime. Results: During 15 193 034 person years of observation (1986-2009), deaths from breast cancer occurred in 1175 women with a diagnosis after being invited to screening and 8996 women who had not been invited before diagnosis. After adjustment for age, birth cohort, county of residence, and national trends in deaths from breast cancer, the mortality rate ratio associated with being invited to mammography screening was 0.72 (95% confidence interval 0.64 to 0.79). To prevent one death from breast cancer, 368 (95% confidence interval 266 to 508) women would need to be invited to screening. Conclusion: Invitation to modern mammography screening may reduce deaths from breast cancer by about 28%

Evaluating the performance of hydrological models via cross-spectral analysis: case study of the Thames Basin, United Kingdom

Nine distributed hydrological models, forced with common meteorological inputs, simulated naturalised daily discharge from the Thames Basin for 1963-2001. While model-dependent evaporative losses are critical for modelling mean discharge, multiple physical processes at many time scales influence the variability and timing of discharge. Here we advocate the use of cross-spectral analysis to measure how the average amplitude, and independently the average phase, of modelled discharge differ from observed discharge at daily to decadal time scales. Simulation of the spectral properties of the model discharge via numerical manipulation of precipitation confirms that modelled transformation involves runoff generation and routing that amplify the annual cycle, while subsurface storage and routing of runoff between grid boxes introduces most autocorrelation and delays. Too much or too little modelled evaporation affects discharge variability as do the capacity and time constants of modelled stores. Additionally the performance of specific models would improve if four issues were tackled: a) non-sinusoidal annual variations in model discharge (prolonged low baseflow and shortened high baseflow, 3 models), b) excessive attenuation of high frequency variability (3 models), c) excessive short-term variability in winter half years but too little variability in summer half years (2 models) and d) introduction of phase delays at the annual scale only during runoff generation (3 models) or only during routing (1 model). Cross-spectral analysis reveals how re-runs of one model using alternative methods of runoff generation - designed to improve performance at the weekly to monthly time scales - degraded performance at the annual scale. The cross-spectral approach facilitates hydrological model diagnoses and development

Multilingual gendered identities: female undergraduate students in London talk about heritage languages

In this paper I explore how a group of female university students, mostly British Asian and in their late teens and early twenties, perform femininities in talk about heritage languages. I argue that analysis of this talk reveals ways in which the participants enact ‘culturally intelligible’ gendered subject positions. This frequently involves negotiating the norms of ‘heteronormativity’, constituting femininity in terms of marriage, motherhood and maintenance of heritage culture and language, and ‘girl power’, constituting femininity in terms of youth, sassiness, glamour and individualism. For these young women, I ask whether higher education can become a site in which they have the opportunities to explore these identifications and examine other ways of imagining the self and what their stories suggest about ‘doing being’ a young British Asian woman in London

Can restorative practices in schools make a difference?

Schools in the UK looking for solutions to concerns about indiscipline have been enthused by the basic premise of restorative practice; the need to restore good relationships when there has been conflict or harm; and develop a school ethos, policies and procedures that reduce the possibilities of such conflict and harm arising. In 2004 the Scottish Executive funded a national pilot project on restorative practice and commissioned a team at Glasgow and Edinburgh Universities to carry out a two-year evaluation of the pilot. In this paper, we discuss staff and pupil understandings and offer some exploration of the underpinning principles of restorative practice as it has developed thus far in schools. We explore the successes and challenges schools experienced and discuss the potential contribution of restorative practices for schools in challenging times. Finally we relate our findings to some critical arguments about the meaning and purposes of discipline and control in schooling

Anpassung und Eignung von Composite Cross Winterweizen- Populationen und anderen Phänotypen auf unterschiedliche N-Versorgungsniveaus

Evolutionary breeding through the introduction of composite cross populations (CCPs)encourages intra-specific diversity and increases diversity in the agricultural landscape. Increased genetic diversity in crops may help to buffer both biotic and abiotic stresses and will play an increasingly important role in unpredictable climatic conditions become more unpredictable (Østergård et al. 2009). A number of CC winter wheat populations, commercial varieties and CCP line selections were tested at the University of Kassel, under differing N input levels in order to test past adaptation and suitability to differing fertilisation levels. The CCPs have been managed since 2005 under both organic and conventional conditions without conscious selection. Genetically diverse CCPs have the ability to be able to adapt to their environment, which may also include the management system, meaning that CCPs grown under conventional conditions may be better suited to growing conditions with higher N inputs, in comparison to CCPs, which have been managed organically under low N levels

The future for global water assessment

The global water cycle is a fundamental component of our climate and Earth system. Many, if not the majority, of the impacts of climate change are water related. We have an imperfect description and understanding of components of the water cycle. This arises from an incomplete observation of some of the stores and fluxes in the water cycle (in particular: precipitation, evaporation, soil moisture and groundwater), problems with the simulation of precipitation by global climate models and the wide diversity of global hydrological models currently in use. This paper discusses these sources of errors and, in particular, explores the errors and advantages of bias correcting climate model outputs for hydrological models using a single large catchment as an example (the Rhine). One conclusion from this analysis is that bias correction is necessary and has an impact on the mean flows and their seasonal cycle. However choice of hydrological model has an equal, if not larger effect on the quality of the simulation. The paper highlights the importance of improving hydrological models, which run at a continental and global scale, and the importance of quantifying uncertainties in impact studies

Structure and play: rethinking regulation in the higher education sector

This paper explores possible tactics for academics working within a context of increasing regulation and constraint. One suggested tactic is to move outside of a creativity-conformity binary. Rather than understanding creativity and conformity as separable, where one is seen as excluding the other, the authors consider the potential of examining the relationships between them. The theme of 'structure and play' illustrates the argument. In the first part of the paper, using various examples from art and design - fields generally associated with creativity - the authors explore the interrelatedness of creativity and conformity. For example, how might design styles, which are generally understood as creative outcomes, constrain creativity and lead to conformity within the design field? Is fashion producing creativity or conformity? Conversely, the ways in which conformity provides the conditions for creativity are also examined. For example, the conformity imposed by the state on artists in the former communist bloc contributed to a thriving underground arts movement which challenged conformity and state regulation. Continuing the theme of 'structure and play', the authors recount a story from an Australian university which foregrounds the ongoing renegotiation of power relations in the academy. This account illustrates how programmatic government in a university, with its aim of regulating conduct, can contribute to unanticipated outcomes. The authors propose that a Foucauldian view of distributed power is useful for academics operating in a context of increasing regulation, as it brings into view sites where power might begin to be renegotiated