Genome-Wide Assessments Reveal Extremely High Levels of Polymorphism of Two Active Families of Mouse Endogenous Retroviral Elements

Endogenous retroviral elements (ERVs) in mice are significant genomic mutagens, causing ∼10% of all reported spontaneous germ line mutations in laboratory strains. The majority of these mutations are due to insertions of two high copy ERV families, the IAP and ETn/MusD elements. This significant level of ongoing retrotranspositional activity suggests that inbred mice are highly variable in content of these two ERV groups. However, no comprehensive genome-wide studies have been performed to assess their level of polymorphism. Here we compared three test strains, for which sufficient genomic sequence is available, to each other and to the reference C57BL/6J genome and detected very high levels of insertional polymorphism for both ERV families, with an estimated false discovery rate of only 0.4%. Specifically, we found that at least 60% of IAP and 25% of ETn/MusD elements detected in any strain are absent in one or more of the other three strains. The polymorphic nature of a set of 40 ETn/MusD elements found within gene introns was confirmed using genomic PCR on DNA from a panel of mouse strains. For some cases, we detected gene-splicing abnormalities involving the ERV and obtained additional evidence for decreased gene expression in strains carrying the insertion. In total, we identified nearly 700 polymorphic IAP or ETn/MusD ERVs or solitary LTRs that reside in gene introns, providing potential candidates that may contribute to gene expression differences among strains. These extreme levels of polymorphism suggest that ERV insertions play a significant role in genetic drift of mouse lines

Genome-Wide Assessments Reveal Extremely High Levels of Polymorphism of Two Active Families of Mouse Endogenous Retroviral Elements

Endogenous retroviral elements (ERVs) in mice are significant genomic mutagens, causing ∼10% of all reported spontaneous germ line mutations in laboratory strains. The majority of these mutations are due to insertions of two high copy ERV families, the IAP and ETn/MusD elements. This significant level of ongoing retrotranspositional activity suggests that inbred mice are highly variable in content of these two ERV groups. However, no comprehensive genome-wide studies have been performed to assess their level of polymorphism. Here we compared three test strains, for which sufficient genomic sequence is available, to each other and to the reference C57BL/6J genome and detected very high levels of insertional polymorphism for both ERV families, with an estimated false discovery rate of only 0.4%. Specifically, we found that at least 60% of IAP and 25% of ETn/MusD elements detected in any strain are absent in one or more of the other three strains. The polymorphic nature of a set of 40 ETn/MusD elements found within gene introns was confirmed using genomic PCR on DNA from a panel of mouse strains. For some cases, we detected gene-splicing abnormalities involving the ERV and obtained additional evidence for decreased gene expression in strains carrying the insertion. In total, we identified nearly 700 polymorphic IAP or ETn/MusD ERVs or solitary LTRs that reside in gene introns, providing potential candidates that may contribute to gene expression differences among strains. These extreme levels of polymorphism suggest that ERV insertions play a significant role in genetic drift of mouse lines

Globalization and risks to health

As borders disappear, people and goods are increasingly free to move, creating new challenges to global health. These cannot be met by national governments alone but must be dealt with instead by international organizations and agreement

Chancen und Grenzen einer Friedenserziehung im Schulfach Politik/Sozialkunde

Frieden ist eine wichtige Kategorie der politischen Bildung. Der Beitrag versucht, den fachlichen Beitrag des Schulfachs Politik/Sozialkunde für die Aufgaben der Friedenserziehung zu bestimmen. Der Politikunterricht hat die Aufgabe, den politischen Prozess und das Handeln der Akteure in aktuellen Konfliktfällen zu rekonstruieren und zu interpretieren. Die Anschaulichkeit der Politik wird durch unterrichtliche Fallanalysen erhöht. Der friedenspädagogische Ertrag des Politikunterrichts liegt darin, dass die Schülerinnen und Schüler eine reflektierende Einstellung gegenüber den Akteuren gewinnen können

Assessing the adequacy of postexperimental inquiries in deception research and the factors that promote participant honesty

The primary aim of this research was to assess the adequacy of postexperimental inquiries (PEI) used in deception research, as well as to examine whether mood state, reward, or administering the PEI as a face-to-face interview or computer survey impacts participants\u27 willingness to divulge suspicion or knowledge about a study. We also sought to determine why participants are not always forthcoming on the PEI. Study 1 examined how frequently PEIs are included in research and found that most researchers employing deception do use a PEI. Studies 2 and 3 showed that participants are often unwilling to divulge suspicion or awareness of deception or to admit to having prior knowledge about a study, though offering a reward and completing the PEI on a computer modestly improved awareness and admission rates. Study 4 indicated several reasons why participants may not reveal suspicion or knowledge about a study on the PEI