NIP1/XB51/NECAB3 is a potential substrate of Nek2, suggesting specific roles of Nek2 in Golgi

Nek2 is a mammalian protein kinase structurally homologous to Aspergillus NIMA. We previously observed that the Nek2 protein was localized in multiple sites within a cell in a cell cycle stage-specific manner. Such dynamic behavior of Nek2 allowed us to propose that Nek2 may be a mitotic regulator that is involved in diverse cell cycle events. To better understand the cellular processes in which Nek2 participates, we carried out yeast two-hybrid screening and isolated Nek2-Interacting Protein 1 (NIP1), which has been also named as XB51 and NECAB3. Physical interactions of Nek2 with NIP1 were confirmed. In fact, Nek2 can phosphorylate NIP1 in vivo. Immumostaining experiments revealed that NIP I is a Golgi protein. These results propose a possible involvement of Nek2 in biological processes of the Golgi body, perhaps in relation to the inheritance of Golgi during mitosis or to cell cycle stage-specific regulation of exocytosis. (C) 2003 Elsevier Inc. All rights reserved.X1112sciescopu

Participants’ Experiences of the Qualitative Interview: Considering the Importance of Research Paradigms

We used a multiple-case study to investigate participants’ experiences in interviews from six qualitative studies that differed in interview orientations, designs, methods, participants, and topics. Roulston’s (2010a, 2010b) interview orientation heuristic guided our ‘paradigm-driven’ analysis of participants’ experiences. We found no differences in participants’ articulation of benefits and risks by interview orientation. Participants’ experiences differed based on the opportunity to reflect on their interview experiences, the sensitivity of the topic explored, and the number of interviews conducted. We discuss the implications of our findings for ‘paradigm-driven’ qualitative research and suggest ethical questions qualitative interview researchers can ask to maximize the benefits of their interviews

Using stories to investigate, reflect on and raise social conciousness in a sporting culture

This chapter describes the qualitative approach utilised to investigate relationships of power experienced within formal and informal mentee-mentor relationships associated with the education programme of a sports coaching culture. Denison and Avner (2011) and Cassidy (2010) suggest that power relations within a culture can contribute to conformity, stifle creativity and lead to apathetic, docile practice

Using fictional characters to challenge ageist assumptions and negative stereotyping

Hayes employs the TV situation comedy, The Royle Family, to enable students training to be podiatrists to examine their own and social attitudes towards aging and the elderly. Focusing on the character ‘Nana’, the grandmother in the series, she examines issues such as stereotyping and media representations of the elderly. Hayes reflects on the significance to a profession such as podiatry of identifying personal and professional assumptions about the elderly, and the implications that negative stereotyping can have for the treatment and services that the elderly receive. She illustrate the profound impact that developing students’ critical reflection through analysis of popular texts, alongside the exploration of factual and theoretical material can have on their self-awareness and on their affective responses to this growing group of health service users

Analyzing for Critical Resistance in Narrative Research

This paper details a narrative analysis strategy called critical resistance analysis (CRA). The aim of a CRA is to bring forward the kinds of subjects participants draw on when talking about themselves in narrative interviews and to make explicit how those subjects are resisted and desired. The CRA is distinguished from other narrative analyses of self in that it focuses on resistance in both its structural, anti-hegemonic and ‘poststructural’, self-refusal forms. The latter kind of resistance is what Hoy (2005) refers to as ‘critical’ resistance; the desire to undo oneself. A CRA looks for participant resistance in narrative and antenarrative (Boje, 2001) data. Antenarratives are incomplete stories that are often too fragmented to analyze using traditional narrative methods and can be seen as powerful examples of meaning-making in progress. A CRA newly brings an antenarrative understanding to the study of self in four analytic foci: deconstruction trace, discourse-argument, resistance and intersubjectivity analysis. Together these analytic foci reveal the subjects narrative participants seek (not) to be and afford a more complex understanding of how participants struggle with and against themselves

The Effect of Insulin Signaling on Female Reproductive Function Independent of Adiposity and Hyperglycemia

Physiological states of insulin resistance such as obesity and diabetes have been linked to abnormalities in female reproductive function. However, it is difficult to distinguish the direct effects of impaired insulin signaling from those of adiposity or hyperglycemia because these conditions often coexist in human syndromes and animal models of insulin resistance. In this study, we used lean, normoglycemic mouse lines with differing degrees of hyperinsulinemia and insulin receptor (Insr) expression to dissect the effects of altered insulin signaling on female reproduction. All three mouse lines [Ttr-Insr−/−, Insr+/−, and Insr+/+ (wild type)] are able to maintain fertility. However, the insulin-resistant and hyperinsulinemic mice demonstrate altered duration of estrous cycles as well as aberrant distribution and morphology of ovarian follicles. These effects appear to be independent of hyperandrogenism in the mice. Pregnancy studies indicate decreased success in early progression of gestation. In successful pregnancies, decreased embryo weights and increased placental calcification also implicate altered insulin signaling in later gestational effects. Thus, abnormal insulin signaling, independent of adipose tissue mass, adipokine expression levels, and hyperglycemia, can affect parameters of the female hypothalamic-pituitary-gonadal axis and pregnancy outcomes