Clinical and Histopathological Diagnosis of Synovial Pad Proliferation in a Racehorse

Synovial pad proliferation that origins from a soft tissue observes in the fetlock joint of horses, mainly racing horses. In the present study, we report the clinical and histopathological findings of synovial pad proliferation in a 15-years-old male horse with mild lameness. A palpable and visible mass was found at the dorsolateral surface of the right hindlimb joint associated with ulcer and hemorrhage. The radiology examination and biopsy sample were performed for the present case. In radiology, there was not exostosis or a bone fraction. Histopathology dominantly demonstrated densely collagenous, fibrous connective tissue and vascular congestion to gather with mild chronic inflammation and fibrosis. No evidence of edema and acute inflammation were found on the tissue sections. The surgical removal of the proliferative mass was suggested for the treatment of choice for optimal recovery

NATURAL CRYPTOSPORIDIUM INFECTION OF THE STOMACH AND INTESTINE IN LABORATORY MICE

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Carbenoxolone Could Deteriorate Streptozotocin-induced Diabetes through Induction of Heat Shock Protein 70 and IFN-γ in C57BL/6 Mice

Type 1 diabetes (T1D), a spontaneous autoimmune disease, is associated with destruction of insulin-producing β-cells in the pancreas. Since some heat shock proteins (HSP), such as HSP70 exert a protective effect in both tissues and cells, the present study was conducted to elucidate the effects of carbenoxolone (CBX) as an HSP70 inducer on T1D.&#x0D; The disease was induced in male C57BL/6 mice using streptozotocin (STZ) and subjects were allocated to therapeutic 1 and therapeutic 2 groups, as well as negative and positive control groups. The treated mice (therapeutic 1 and therapeutic 2 groups) received 50 mg/kg CBX intraperitoneally every 24 hours, in the therapeutic 1  group the drug was injected before and after disease induction whereas in the therapeutic 2 group the drug was injected only after disease induction. Serum fasting blood sugar (FBS) level, cytokines production (Interferon-gamma (IFN-γ), Interleukin 10 (IL-10), and IL-17), serum HSP70 level and CD4+CD25+Foxp3+ regulatory T cell (Treg) frequency measurements were outperformed 14 days after the last STZ injection.&#x0D; Our results showed that in the treated groups, serum HSP70, IFN-γ, and IL-17 levels were increased in contrast to the untreated groups. The IL-10 level was markedly decreased in comparison to untreated diabetic mice (p&lt;0.05). Moreover, it was found that the frequency of Tregs in treated mice was lower in comparison to the untreated mice but the difference was not significant (p&gt;0.05).&#x0D; Our results confirm that CBX might through HSP70 induction, followed by increasing IFN-γ level leads to suppression of IL-10 production in diabetic mice resulted in toxic effects on pancreatic islet beta cells and deteriorating of disease.&#x0D;  </jats:p

Effects of Administration of Monosodium Glutamate and Methylphenidate on the Histologic Structure of Testicular Tissue and Microscopic Indices of Spermatogenesis in Adolescent Rats

Introduction: Methylphenidate (MPH) is one of the common medications used for maintaining alertness and improving attention. Monosodium glutamate (MSG) is a food additive, which acts as an enhancer of palatability. The aim of this study was to evaluate the various effects of these compounds on the reproductive system during adolescence.   Materials and Methods: Methylphenidate (5 and 10 mg/kg) and monosodium glutamate (6 and 60 mg/kg) were administrated to adolescent rats. After 60 days, the pituitary-testicular axis hormones were assayed and testicular histomorphometric studies were performed.   Findings: The coadministration of MPH (5 mg/kg) and MSG (60 mg/kg) led to elevation in serum FSH levels (P0.05). The administration of MSG led to a reduction in the population of Sertoli cells, spermatogonia, and spermatocyte. Coadministration of MPH and MSG also reduced this population. Morphometric studies revealed decrement of tubular diameter and germinal epithelium height (P<0.05), especially with low doses of MPH and high doses of MSG. The changes in spermatogenic indices were similar to morphometric results. Tubular atrophy, interstitial edema, and depopulation of spermatogenic cells were observed in MPH and MSG treated groups. Coadministration of these compounds increased spermatids with pyknotic nucleus.   Conclusion: It has been concluded that the coadministration of MPH and MSG through the induction of some hormonal and structural alterations could induce some changes in the normal structure and function of the reproductive system

Case Report. The first report of pyrrolizidine alkaloid poisoning in a gazelle (Gazella Subgutturosa) – histopathologic diagnosis

Abstract Pyrrolizidine alkaloids (PAs) are natural phytotoxins found in thousands of plant species around the world. They are probably the most common poisonous plants affecting livestock, wildlife and humans. The disease occurs almost entirely as a consequence of chronic poisoning and in general ends fatally. In the present study, PAs poisoning was investigated in a gazelle with hepatic encephalopathy associated with severe neurologic signs. The main clinical signs included head pressing, progressive depression and weakness, ataxia and reluctance to move, turn the head to the left and to paddle, hyperesthesia and decreased food intake. Histopathological examination revealed major lesions in the liver consisting of severe hepatocyte megalocytosis and hypertrophy with nuclei enlargement, mild bile duct hyperplasia, centriacinar fatty change and hepatocellular necrosis. Moreover, pulmonary congestion and edema with endothelium necrosis and alveolar septa thickening, severe congestion in vessels of the brain and meninges, and myocardial necrosis were observed.</jats:p